Mechanical Stretch Induces Activation of Skeletal Muscle Satellite Cells in Vitro

Tatsumi, Ryuichi; Sheehan, Shannon M.; Iwasaki, Hiroshi; Hattori, Akihito; Allen, Ronald E.

doi:10.1006/excr.2001.5252

Cited by 186 publications

(204 citation statements)

References 18 publications

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“…Stretch-conditioned medium plus anti-HGF antibody (d) did not stimulate activation relative to the control, and the addition of HGF negated the neutralizing effect of anti-HGF (e). These results are consistent with results from Tatsumi et al (2001) and serve as important controls for the other treatments within this experiment.…”

Section: Resultssupporting

confidence: 89%

“…Figure 7B displays results of immunoblots analyzing the presence of HGF in medium from the experiment. These immunoblots indicate that the ␣ subunit of HGF (bands in the 60-kDa range) was not found in medium from unstretched cultures (lane a), but was present in medium from stretched cultures (lane b), as seen previously (Tatsumi et al, 2001). Cultures stretched in the presence of d-NAME (lane f) or l-Arg (lane g) also released HGF into medium, but cells stretched in the presence of l-NAME (lane h) had diminished amounts of HGF in medium.…”

Section: Resultssupporting

confidence: 80%

“…In previous work, we used a FlexerCell system to apply cyclic stretch to isolated rat satellite cells at 12 h postplating and found that stretched satellite cells were activated sooner than unstretched control satellite cells (Tatsumi et al, 2001). Furthermore, we found that the activation response was due to HGF release from its extracellular association with satellite cells.…”

Section: Introductionmentioning

confidence: 74%

“…The percentage of stretch and the stretch interval length were optimized for satellite cell activation previously at 25% stretch at 12-s intervals. Also, the viability of cells, as measured by cell density at the end of 36 h of stretch treatment, was shown to be the same for stretched and unstretched cells (Tatsumi et al, 2001). …”

Section: Satellite Cell Isolation and Culturementioning

confidence: 92%

“…Conditioned media from 2-h stretched cells were subjected to SDS-PAGE on 10% polyacrylamide gels under reducing conditions (Tatsumi et al, 2001). Separated proteins were transferred to nitrocellulose membranes, which were then blocked with 10% powdered milk in 0.1% Tween 20/Tris-buffered saline (TTBS) before incubation with 1:500 dilution of anti-HGF antibody overnight at room temperature.…”

Section: Immunoblotting and Enhanced Chemiluminescence (Ecl)mentioning

confidence: 99%

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Release of Hepatocyte Growth Factor from Mechanically Stretched Skeletal Muscle Satellite Cells and Role of pH and Nitric Oxide

Tatsumi

Hattori

Ikeuchi

et al. 2002

MBoC

233

220

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Application of mechanical stretch to cultured adult rat muscle satellite cells results in release of hepatocyte growth factor (HGF) and accelerated entry into the cell cycle. Stretch activation of cultured rat muscle satellite cells was observed only when medium pH was between 7.1 and 7.5, even though activation of satellite cells was accelerated by exogenous HGF over a pH range from 6.9 to 7.8. Furthermore, HGF was only released in stretched cultures when the pH of the medium was between 7.1 and 7.4. Conditioned medium from stretched satellite cell cultures stimulated activation of unstretched satellite cells, and the addition of anti-HGF neutralizing antibodies to stretch-conditioned medium inhibited the stretch activation response. Conditioned medium from satellite cells that were stretched in the presence of nitric-oxide synthase (NOS) inhibitor N -nitrol-arginine methyl ester hydrochloride did not accelerate activation of unstretched control satellite cells, and HGF was not released into the medium. Conditioned medium from unstretched cells that were treated with a nitric oxide donor, sodium nitroprusside dihydrate, was able to accelerate the activation of satellite cells in vitro, and HGF was found in the conditioned medium. Immunoblot analysis indicated that both neuronal and endothelial NOS isoforms were present in satellite cell cultures. Furthermore, assays of NOS activity in stretched satellite cell cultures demonstrated that NOS is stimulated when satellite cells are stretched in vitro. These experiments indicate that stretch triggers an intracellular cascade of events, including nitric oxide synthesis, which results in HGF release and satellite cell activation.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 74%

Section: Satellite Cell Isolation and Culturementioning

confidence: 92%

Section: Immunoblotting and Enhanced Chemiluminescence (Ecl)mentioning

confidence: 99%

See 3 more Smart Citations

Release of Hepatocyte Growth Factor from Mechanically Stretched Skeletal Muscle Satellite Cells and Role of pH and Nitric Oxide

Tatsumi

Hattori

Ikeuchi

et al. 2002

MBoC

233

220

View full text Add to dashboard Cite

show abstract

Muscle Tissue Engineering Approaches

Hoff

Grefte

2013

Stem Cells in Craniofacial Development and Regeneration

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Piezoelectric Nano‐Biomaterials for Biomedicine and Tissue Regeneration

Kapat

Shubhra

Zhou

et al. 2020

Adv Funct Materials

287

209

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Among various classes of biomaterials, the majority of non-centrosymmetric crystalline materials exhibit piezoelectric properties, i.e., the accumulation of charge in response to applied mechanical stress or deformation. Due to the growing interest in nanomaterials, piezoelectric nano-biomaterials have been widely investigated, leading to remarkable advancements throughout the last two decades. Piezoelectric properties, high surface energy, targeting properties, and intricate cell-material interactions render piezoelectric nanomaterials highly attractive for application in therapeutics as well as regenerative medicine. Herein, the major focus is to highlight the wide range of applications of piezoelectric nano-biomaterials in drug delivery, theranostics, and tissue regeneration. After a brief introduction to piezoelectricity, an overview is provided on the major classes of piezoelectric biomaterials as well as a description of the origin of biopiezoelectricity in different tissues and macromolecules. Subsequently, relevant properties and postfabrication strategies of nanostructured piezoelectric biomaterials are discussed aiming to maximize piezoresponse. Finally, recent studies on nano-piezoceramics and piezopolymers are presented, with specific focus on barium titanate, zinc oxide, and polyvinylidene fluoride.

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Mechanical Stretch Induces Activation of Skeletal Muscle Satellite Cells in Vitro

Cited by 186 publications

References 18 publications

Release of Hepatocyte Growth Factor from Mechanically Stretched Skeletal Muscle Satellite Cells and Role of pH and Nitric Oxide

Release of Hepatocyte Growth Factor from Mechanically Stretched Skeletal Muscle Satellite Cells and Role of pH and Nitric Oxide

Muscle Tissue Engineering Approaches

Piezoelectric Nano‐Biomaterials for Biomedicine and Tissue Regeneration

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