Mechanical stretch aggravates aortic dissection by regulating MAPK pathway and the expression of MMP-9 and inflammation factors

Hu, Yunnan; Lü, Lin; Qiu, Zhihuang; Huang, Qiuyu; Chen, Yinhai; Chen, Liangwan

doi:10.1016/j.biopha.2018.09.129

Cited by 29 publications

(18 citation statements)

References 45 publications

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“…The maintenance of vessel homeostasis relies on the normal contractile function of vascular smooth muscle cells, which is closely related to cell-matrix adhesion. The extracellular mechanical signal (blood stimulation) is converted into intracellular chemical signal via matrix-membrane integrin-focal adhesion pathway, which can further activate the polymerization of downstream actin, stabilize the cytoskeleton, and maintain the plasticity of blood vessel [ 24 ]. Therefore, mutations in genes encoding cell-matrix adhesion-related proteins (FBN-1, MFAP5, and COL3A1) can lead to AAD [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

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Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics

Wang

Yang

Tang

et al. 2020

BioMed Research International

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Acute aortic dissection (AAD) is an emergent vascular disease. Currently, its diagnosis depends on clinical and radiological investigations but lacking of serum biomarkers. In this study, we aimed to identify potential serum biomarkers for AAD using label-free proteomics approach. A total of 90 serum samples were collected from three groups: patients with acute aortic dissection (AAD, n=30), patients with acute myocardial infarction (AMI, n=30), and healthy controls (n=30), and the first four samples from each group were selected for label-free proteomics analysis. Using label-free approach, a total of 22 differentially expressed proteins were identified in the serum samples of the AAD group, of which 15 were upregulated and 7 were downregulated as compared to the AMI and healthy control groups. The most prominent increased protein was vinculin, which was selected to validate in total samples. The level of vinculin was significantly elevated in AAD patients (15.8 ng/ml, IQR: 9.3-19.9 ng/ml) than that in AMI patients (8.6 ng/ml, IQR:5.3-11.4 ng/ml) and healthy volunteers (5.3 ng/ml, IQR:2.8-7.6 ng/ml), P<0.0001. Furthermore, the concentration of vinculin both increased in type A and B dissection. At the early stage of AAD, vinculin maintained a high level to 48 hours compared with that of AMI. Our study demonstrated that vinculin may play a role in the early diagnosis of AAD.

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Section: Discussionmentioning

confidence: 99%

“…focal adhesion pathway, which can further activate the polymerization of downstream actin, stabilize the cytoskeleton, and maintain the plasticity of blood vessel [24]. Therefore, mutations in genes encoding cell-matrix adhesion-related proteins (FBN-1, MFAP5, and COL3A1) can lead to AAD [25].…”

Section: Biomed Research Internationalmentioning

confidence: 99%

Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics

Wang

Yang

Tang

et al. 2020

BioMed Research International

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“…The inflammatory microenvironment can increase the mutation rate of cells and provide support for tumor initiation ( 24 , 35 ). It has been indicated that the IL family can upregulate MMP-9 expression through the infiltration and recruitment of Th helper 17 cells to inflammatory cells, thus promoting tumor angiogenesis and serving an important role in the process of tumor invasion and metastasis ( 36 ). Other factors, such as IL-6, are involved in the transformation of epithelial stroma, and can influence the occurrence and development of tumors by reacting to alterations in the tumor microenvironment ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of vascular‑associated factors and the prognosis of poorly differentiated hypopharyngeal carcinoma

et al. 2020

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The aim of the present study was to analyze the characteristics of angiogenic factors in patients with hypopharyngeal cancer, and to study the effects of these factors on induction chemotherapy, patient prognosis and survival. Data from 60 eligible patients with hypopharyngeal cancer that were treated between January 2012 and December 2016 were collected retrospectively. The differential expression of angiogenic factors in tumor and peritumoral tissues was analyzed retrospectively to assess the association between five differentially expressed genes, including interleukin (IL)-1β, transforming growth factor (TGF)-β, matrix metalloproteinase-9 (MMP-9), angiopoietin-2 and interferon-inducible T-cell α chemoattractant, and clinicopathological characteristics in different types of chemotherapy-associated blood vessels within samples of poorly differentiated hypopharyngeal cancer. The χ 2 test or t-test was used to compare the frequency data, the Kaplan-Meier method was used for survival analysis and the log-rank test was used to compare the Kaplan-Meier curves. P<0.05 was considered to indicate a statistically significant difference. The results of the present study demonstrated that there was a significant difference in the expression levels of vascular-associated factors between hypopharyngeal carcinoma and peritumoral tissues. Additionally, the results revealed a significant difference in the overall survival and prognosis of patients with a decreased vascular classification compared with patients with an unchanged vascular classification, which was assessed using narrowband imaging (NBI) following induction chemotherapy (P<0.05). The results of single factor analysis indicated that IL-1β, TGF-β and MMP-9 were associated with decreased blood vessel classification (P<0.05). In conclusion, IL-1β, TGF-β and MMP-9 may be used as predictors of the effect of induction chemotherapy on poorly differentiated hypopharyngeal cancer. Therefore, when patients with advanced hypopharyngeal cancer undergo chemotherapy, NBI vascular examination and screening for associated vascular factors should be performed before and after chemotherapy. Alterations in vascular classification, assessed using NBI, and abnormal expression of vascular factors may also be used as reference factors for prognosis.

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“…Studies also testified that mechanical stretching can activate the phosphorylation of the MAPK pathway [28,29]. Also, studies have shown that the expression of MMP-9 and TGF-β1 in tissues may be related to stretch-activated channel (SAC) and mitogen-activated protein kinase (MAPK) signaling pathway [12,30,31]. Therefore, we speculated that SAC and MAPK signaling pathway may be potential regulatory molecules in PH-LHD.…”

Section: Introductionmentioning

confidence: 89%

“…MAPK pathway is proven to be involved in various physiological processes, such as cell growth, development and inter-cell function synchronization, which also can be closely related to the regulation of inflammation and stress response [26,27]. Studies also testified that mechanical stretching can activate the phosphorylation of the MAPK pathway [28,29]. Also, studies have shown that the expression of MMP-9 and TGF-β1 in tissues may be related to stretch-activated channel (SAC) and mitogen-activated protein kinase (MAPK) signaling pathway [12,30,31].…”

Section: Introductionmentioning

confidence: 99%

Mechanical stretching of pulmonary vein stimulates matrix metalloproteinase-9 and transforming growth factor-β1 through stretch-activated channel/MAPK pathways in pulmonary hypertension due to left heart disease model rats

et al. 2020

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Pulmonary hypertension due to left heart disease (PH-LHD) is a momentous pulmonary hypertension disease, and left heart disease is the most familiar cause. Mechanical stretching may be a crucial cause of vascular remodeling. While, the underlining mechanism of mechanical stretching-induced in remodeling of pulmonary vein in the early stage of PH-LHD has not been completely elucidated. In our study, the PH-LHD model rats were successfully constructed. After 25 days, doppler echocardiography and hemodynamic examination were performed. In addition, after treatment, the levels of matrix metalloproteinase-9 (MMP-9) and transforming growth factor-β1 (TGF-β1) were determined by ELISA, immunohistochemistry and western blot assays in the pulmonary veins. Moreover, the pathological change of pulmonary tissues was evaluated by H&E staining. Our results uncovered that left ventricular insufficiency and interventricular septal shift could be observed in PH-LHD model rats, and the right ventricular systolic pressure (RVSP) and mean left atrial pressure (mLAP) were also elevated in PH-LHD model rats. Meanwhile, we found that MMP-9 and TGF-β1 could be highly expressed in PH-LHD model rats. Besides, we revealed that stretch-activated channel (SAC)/mitogen-activated protein kinases (MAPKs) signaling pathway could be involved in the upregulations of MMP-9 and TGF-β1 mediated by mechanical stretching in pulmonary vein. Therefore, current research revealed that mechanical stretching induced the increasing expressions of MMP-9 and TGF-β1 in pulmonary vein, which could be mediated by activation of SAC/MAPKs signaling pathway in the early stage of PH-LHD.

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Mechanical stretch aggravates aortic dissection by regulating MAPK pathway and the expression of MMP-9 and inflammation factors

Cited by 29 publications

References 45 publications

Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics

Identification of Vinculin as a Potential Diagnostic Biomarker for Acute Aortic Dissection Using Label-Free Proteomics

Analysis of vascular‑associated factors and the prognosis of poorly differentiated hypopharyngeal carcinoma

Mechanical stretching of pulmonary vein stimulates matrix metalloproteinase-9 and transforming growth factor-β1 through stretch-activated channel/MAPK pathways in pulmonary hypertension due to left heart disease model rats

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