Mechanical stress induced astaxanthin accumulation of <i>H. pluvialis</i> on a chip

Yao, Junyi; Kim, Hyun Soo; Kim, Jee Young; Choi, Yoon E.; Park, Jaewon

doi:10.1039/c9lc01030k

Cited by 19 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been confirmed that endogenously generated reactive oxygen species (ROS) induced by stress conditions are essential for the transformation of H. pluvialis from the macrozooid (green) stage to the hematocyst (red) stage, which leads to astaxanthin accumulation. − Thus, H. pluvialis cultures have been subjected to various stresses, such as abiotic and chemical treatments, in efforts to increase astaxanthin biosynthesis up to commercially viable levels. − For example, it was found that electrical stimulation improved the growth of H.…”

Section: Introductionmentioning

confidence: 99%

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Wang

Mou

Qin

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

The unicellular freshwater green alga Haematococcus pluvialis has attracted much research attention due to its biosynthetic ability for large amounts of astaxanthin, a blood-red ketocarotenoid that is used in cosmetics, nutraceuticals, and pharmaceuticals. Recently, numerous studies have investigated the functions of natural astaxanthin; however, the high cost of the production of astaxanthin from H. pluvialis cultures restricts its commercial viability. There is an urgent need to fulfill commercial demands by increasing astaxanthin accumulation from H. pluvialis cultures. In this study, we discovered that treatment of H. pluvialis cultures at the beginning of the macrozooid stage (day 0) with 1 μM rac-GR24, a synthetic analogue of strigolactones (a class of phytohormones), led to significant increases in biomass [up to a maximum dry cell weight (DCW) of 0.53 g/L] during the macrozooid stage and astaxanthin (from 0.63 to 5.32% of DCW) during the hematocyst stage. We elucidated that this enhancement of biomass accumulation during the macrozooid stage by rac-GR24 is due to its increasing CO 2 utilization efficiency in photosynthesis and carbohydrate biosynthesis. We also found that rac-GR24 stimulated the overproduction of nicotinamide adenine dinucleotide phosphate (NADPH) and antioxidant enzymes in H. pluvialis cultures, which alleviated the oxidative damage caused by reactive oxygen species generated during the hematocyst stage due to the exhaustion of nitrogen supplies. Moreover, rac-GR24 treatment of H. pluvialis synergistically altered the activity of the pathways of fatty acid biosynthesis and astaxanthin esterification, which resulted in larger amounts of astaxanthin being generated by rac-GR24-treated cultures than by controls. In summary, we have developed a feasible and economic rac-GR24-assisted strategy that increases the amounts of biomass and astaxanthin generated by H. pluvialis cultures, and have provided novel insights into the mechanistic roles of rac-GR24 to achieve these effects.

show abstract

Section: Introductionmentioning

confidence: 99%

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Wang

Mou

Qin

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…As tendências relacionadas ao consumo de alimentos naturais para melhorar a saúde humana estão mudando e o interes-se por esses produtos alimentares estão aumentando. Portanto, a indústria de alimentos está desenvolvendo novos produtos alimentícios enriquecidos com ingredientes funcionais que podem fornecer benefícios à saúde, além dos nutrientes tradicionais (Yao et al, 2020). Dessa forma, as microalgas podem ser de grande relevância, visto que são uma fonte importante de nutrientes funcionais com várias aplicações biotecnológicas em potencial comprovadas (Michalak;Chojnacka, 2015).…”

Section: Potencial Biotecnológico Da Haematococcus Pluvialisunclassified

Technological innovations in the cultivation of Haematococcus pluvialis microalgae: review

Souza¹,

Viana²,

Moreira³

et al. 2020

S&G

View full text Add to dashboard Cite

The cultivation of microalgae presents a great biotechnological potential, mainly to produce natural bioactive substances, which can be used in the pharmaceutical industry and especially in the development of functional foods, thanks to its nutritional properties. Among the commercially important microalgae, Haematococcus pluvialis is considered the main source of natural astaxanthin, a carotenoid of high antioxidant action and with wide applications in the nutraceuticals, cosmetics, food and aquaculture industries. This review aimed to cover the most important aspects of biology, biochemical composition, biosynthesis and astaxanthin accumulation in the cells of H. pluvialis, in addition to its broad application to humans and animals. The methodology used in this work was a systematic review of the literature, presenting the gaps and opportunities for research. This work provided a broader view of the technologies and methodologies used to produce H. pluvialis, providing a direction for future work to be undertaken. During the bibliographic survey, it was observed that information regarding the cultivation of H. pluvialis, aiming at the production of astaxanthin, is still very incipient in Brazil, with results observed only on a laboratory scale, making it difficult to really understand the implementation costs for a possible commercial production. This work has started a larger research and will serve as a basis for future activities, mainly to solve possible doubts.

show abstract

“…Astaxanthin biomolecules accumulate inside neutral lipid bodies forming ester bonds with long-chain fatty acids, enabled by closely interconnected metabolic networks of lipogenesis and carotenogenesis [6]. To improve the astaxanthin productivity of photosynthetic H. pluvialis cultures, various stress-inducing strategies, such as nitrogen starvation [7], strong light irradiance [8], high salinity [9], high temperature [10], mechanical stress [11], electrical treatment [12], and aminoclay nanoparticle addition [2] have been applied. However, these approaches are time-consuming, require high energy input and large amounts of chemicals; additionally, they may substantially inhibit cell growth.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

et al. 2022

View full text Add to dashboard Cite

Improving the production rate of high-value nutraceutical compounds, such as astaxanthin and polyunsaturated fatty acids (PUFAs), is important for the commercialization of Haematococcus pluvialis biorefineries. Here, the effects of a phytohormone, strigolactone analog rac-GR24, on cell growth and astaxanthin and fatty acid biosynthesis in H. pluvialis were investigated. Four concentrations (2, 4, 6, and 8 µM) of rac-GR24 were initially added during 30 days of photoautotrophic cultivation. The addition of rac-GR24 improved cell number density and chlorophyll concentration in H. pluvialis cultures compared to the control; the optimal concentration was 8 µM. Despite a slightly reduced astaxanthin content of 30-d-old cyst cells, the astaxanthin production (26.1 ± 1.7 mg/L) improved by 21% compared to the rac-GR24-free control (21.6 ± 1.5 mg/L), owing to improved biomass production. Notably, at the highest dosage of 8 µM rac-GR24, the total fatty acid content of the treated H. pluvialis cells (899.8 pg/cell) was higher than that of the untreated cells (762.5 pg/cell), resulting in a significant increase in the total fatty acid production (361.6 ± 48.0 mg/L; 61% improvement over the control). The ratio of PUFAs, such as linoleic (C18:2) and linolenic (C18:3) acids, among total fatty acids was high (41.5–44.6% w/w) regardless of the rac-GR24 dose.

show abstract

Mechanical stress induced astaxanthin accumulation of H. pluvialis on a chip

Abstract: A Haematococcus pluvialis culture platform capable of sorting motile vegetative stages cells and culturing the selected cells under controlled mechanical stress to screen the condition that maximizes the astaxanthin accumulation.

Cited by 19 publications

References 37 publications

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation

Technological innovations in the cultivation of Haematococcus pluvialis microalgae: review

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

Contact Info

Product

Resources

About