Mechanical Strain Using 2D and 3D Bioreactors Induces Osteogenesis: Implications for Bone Tissue Engineering

Griensven, Martijn van; Diederichs, Solvig; Roeker, Stefanie; Boehm, Stefanie; Peterbauer, Anja; Wolbank, Susanne; Riechers, Daniel; Stahl, Frank; Kasper, Cornelia

doi:10.1007/978-3-540-69357-4_5

Cited by 22 publications

(19 citation statements)

References 71 publications

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“…Consistently, dynamic compression enhances the vasculogenic activities of circulating endothelial precursors (EPCs) seeded in the demineralized bone matrix scaffolds (Kong et al, 2012). The relationship between mechanical inputs and resulting biological tissue structure, composition, and metabolism is reported and discussed in several other papers and reviews (David et al, 2008;Desmoulin et al, 2013;Gardel, Serra, Reis, & Gomes, 2014;Gaspar, Gomide, & Monteiro;S.-T. Li et al, 2014;van Griensven et al, 2009).…”

Section: The Need For Function: Mechano-electrical Stressmentioning

confidence: 91%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

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Bone plays several physiological functions and is the second most commonly transplanted tissue after blood. Since the treatment of large bone defects is still unsatisfactory, researchers have endeavoured to obtain scaffolds able to release growth and differentiation factors for mesenchimal stem cells, osteoblasts and endothelial cells in order to obtain faster mineralization and prompt a reliable vascularization.Nowadays, the application of osteoblastic cultures spans from cell physiology and pharmacology to cytocompatibility measurement and osteogenic potential evaluation of novel biomaterials. To overcome the simple traditional monocultures in vitro, co-cultures of osteogenic and vasculogenic precursors were introduced with very interesting results. Increasingly complex culture systems have been developed, where cells are seeded on proper scaffolds and stimulated so as to mimic the physiological conditions more accurately. These bioreactors aim at enabling bone regeneration by incorporating different cells types into bioinspired materials within a surveilled habitat.This review is focused on the most recent developments in the organomimetic cultures of osteoblasts and vascular endothelial cells for bone tissue engineering.

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Section: The Need For Function: Mechano-electrical Stressmentioning

confidence: 91%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

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“…A number of studies have proved that different types of loading have different effects on bone cells. These effects depend on the magnitude, duration, frequency and mode of the strain (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Osteogenic response of mesenchymal stem cells to continuous mechanical strain is dependent on ERK1/2-Runx2 signaling

et al. 2012

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Abstract. Mechanical stimuli are responsible for bone remodeling during orthodontic tooth movement. The role of mechanical stimulation in the regulation of the fate of bone mesenchymal stem cells (BMSCs) is of interest in bone regeneration and tissue engineering applications. However, the signaling pathway involved in strain-induced biochemical events in BMSCs is not well established and can be controversial. This study investigated strain-induced proliferation and differentiation of BMSCs, as well as the mechanism of mechanotransduction. BMSCs were exposed to continuous mechanical strain (CMS) of 10% at 1 Hz. The results showed that CMS reduced the proliferation of BMSCs and stimulated osteogenic differentiation by activating Runx2, followed by increased alkaline phosphatase (ALP) activity and mRNA expression of osteogenesis-related genes (ALP, collagen type I and osteocalcin). Furthermore, the phosphorylation level of extracellular regulated protein kinase (ERK)1/2 increased significantly at the onset of strain. However, the presence of U0126, a selective inhibitor of ERK1/2, blocked the induction of Runx2 and subsequent osteogenic events. These findings demonstrate that CMS regulated Runx2 activation and favored osteoblast differentiation through activation of the ERK1/2 signaling pathway. These results will contribute to a better understanding of strain-induced bone remodeling and will form the basis for the correct choice of applied force in orthodontic treatment.

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“…12 Perfusion has also been coupled with electrical stimulation for cardiac tissue engineering. 13 Mechanical strain is also known to be a potent factor in affecting cell function in a variety of tissues, 14,15 including cardiovascular tissues. 16,17 In these and other studies, strain has been shown to facilitate cell remodeling of 3D artificial tissues and have important effects on gene expression and cell function.…”

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confidence: 99%

Simultaneous Application of Interstitial Flow and Cyclic Mechanical Strain to a Three-Dimensional Cell-Seeded Hydrogel

Galie

Stegemann²

2011

Tissue Engineering Part C: Methods

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The present study describes the design and validation of a simple apparatus to apply simultaneous mechanical and fluidic stress to three-dimensional (3D) cell-seeded collagen hydrogels. Constructs were formed in wells in a silicone substrate that could be stretched cyclically, and were also fitted with inlet ports to apply fluid flow. Acid etching was used to retain adhesion of the gels to the walls of the well, and an acellular layer of collagen hydrogel was used to distribute flow evenly. Finite element modeling showed that 5% uniaxial strain applied to the entire silicone substrate resulted in *6.5% strain in each of the gel constructs. Permeability testing and flow observation showed that acellular hydrogels were fourfold more permeable than cardiac fibroblast-seeded gels, and that the fluid distributed evenly in the acellular layer before entering the cell-seeded gel. Viability testing and imaging demonstrated that cells remained viable with expected fibroblast morphology for the 120 h duration of the experiments. These results demonstrate that this simple bioreactor can be used to study the effects of mechanical strain and interstitial flow in 3D protein hydrogels. Such 3D tissue models have utility in studying cell and tissue responses to their mechanical environment.

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Mechanical Strain Using 2D and 3D Bioreactors Induces Osteogenesis: Implications for Bone Tissue Engineering

Cited by 22 publications

References 71 publications

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

Osteogenic response of mesenchymal stem cells to continuous mechanical strain is dependent on ERK1/2-Runx2 signaling

Simultaneous Application of Interstitial Flow and Cyclic Mechanical Strain to a Three-Dimensional Cell-Seeded Hydrogel

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