Mechanical Stimuli-induced Urothelial Differentiation in a Human Tissue-engineered Tubular Genitourinary Graft

Cattan, Valérie; Bernard, Geneviève; Rousseau, Alexandre Fontaine; Bouhout, Sara; Chabaud, Stéphane; Auger, François A.; Bolduc, Stéphane

doi:10.1016/j.eururo.2011.05.051

Cited by 58 publications

(53 citation statements)

References 25 publications

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“…Possible regulatory stimuli for these changes could be manifold, but may be most likely attributed to a local mechanical stimulus as a marked change of epithelial cell morphology occurs in parallel to milk accumulation. Therefore, it is likely that mechanical stress may change TJ composition, which is also discussed, e.g., for urothelia induced by the filling (and rapid emptying) of the urinary bladder [11] and in wound healing models [20].…”

Section: Alveoli During Regular and Interrupted Sucklingmentioning

confidence: 99%

Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice

Markov

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Fomina

et al. 2011

Pflugers Arch - Eur J Physiol

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Milk production is modulated by the paracellular barrier function of tight junction (TJ) proteins located in the mammary epithelium. The aim of our study was the molecular analysis of TJs in native lactating murine mammary gland epithelium as this process may strongly challenge epithelial barrier properties and regulation. Mammary gland tissue specimens from lactating control mice and animals after a 20-h interruption of suckling were prepared; histological analyses were performed by light and electron microscopy; and expression of TJ proteins was detected by PCR, Western blotting, immunofluorescent staining, and confocal laser scanning microscopy. Discontinuation of suckling resulted in a substantial accumulation of milk in mammary glands, an increase of alveolar size, and a flattening of epithelial cells without effects on inflammatory indicators. In control tissues, PCR and Western blots showed signals for occludin, and claudin-1, -2, -3, -4, -5, -7, -8, -15, and -16. After a 20-h accumulation of milk, expression of two sealing TJ proteins, claudin-1 and -3, was markedly increased, whereas two TJ proteins involved in cation transport, claudin-2 and -16, were reduced. Real-time PCR validated increased transcripts of claudin-1 and claudin-3. During extension of mammary glands in the process of lactation, claudin-1 and -3 are markedly induced and claudin-2 and -16 are decreased. Volume and composition of milk might be strongly dependent on this counter-regulation of sealing claudins with permeability-mediating claudins, indicating a physiological process of a tightening of TJs against a back-leak of solutes and ions from the alveolar lumen.

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Section: Alveoli During Regular and Interrupted Sucklingmentioning

confidence: 99%

Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice

Markov

Круглова

Fomina

et al. 2011

Pflugers Arch - Eur J Physiol

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“…Mechanical characteristics of this model are roughly similar or even better than the ones of the native tissue [51]. Tubes were placed in constant flow to stimulate the differentiation of the seeded urothelial cells [48]. Evidence of terminal differentiation of urothelial cells including uroplakins was expressed and formed near native structures (Figure 4).…”

Section: Tissue Engineering Of Urethramentioning

confidence: 99%

Tissue Engineering of Urinary Bladder and Urethra: Advances from Bench to Patients

Orabi

Bouhout

Morissette

et al. 2013

The Scientific World Journal

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Urinary tract is subjected to many varieties of pathologies since birth including congenital anomalies, trauma, inflammatory lesions, and malignancy. These diseases necessitate the replacement of involved organs and tissues. Shortage of organ donation, problems of immunosuppression, and complications associated with the use of nonnative tissues have urged clinicians and scientists to investigate new therapies, namely, tissue engineering. Tissue engineering follows principles of cell transplantation, materials science, and engineering. Epithelial and muscle cells can be harvested and used for reconstruction of the engineered grafts. These cells must be delivered in a well-organized and differentiated condition because water-seal epithelium and well-oriented muscle layer are needed for proper function of the substitute tissues. Synthetic or natural scaffolds have been used for engineering lower urinary tract. Harnessing autologous cells to produce their own matrix and form scaffolds is a new strategy for engineering bladder and urethra. This self-assembly technique avoids the biosafety and immunological reactions related to the use of biodegradable scaffolds. Autologous equivalents have already been produced for pigs (bladder) and human (urethra and bladder). The purpose of this paper is to present a review for the existing methods of engineering bladder and urethra and to point toward perspectives for their replacement.

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“…A gradient of increasing fluid shearing force (2–6 mN) was applied to improve adhesion and ordered arrangement of seeding cells, in addition to the direct effect of promoting cell proliferation (Fig. 3B) [36]. Thus, a transition of epithelial cells from 2-dimensional growth to 3-dimensional growth on the APCS scaffold was implemented.…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of Auto-Tissue-Engineered Lamellar Cornea by Dynamic Culture for Transplantation: A Rabbit Model

Zhou

Wang

et al. 2014

PLoS ONE

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To construct an auto-tissue-engineered lamellar cornea (ATELC) for transplantation, based on acellular porcine corneal stroma and autologous corneal limbal explants, a dynamic culture process, which composed of a submersion culture, a perfusion culture and a dynamic air-liquid interface culture, was performed using appropriate parameters. The results showed that the ATELC-Dynamic possessed histological structure and DNA content that were similar to native lamellar cornea (NLC, p>0.05). Compared to NLC, the protein contents of zonula occludens-1, desmocollin-2 and integrin β4 in ATELC-Dynamic reached 93%, 89% and 73%, respectively. The basal cells of ATELC-Dynamic showed a better differentiation phenotype (K3−, P63+, ABCG2+) compared with that of ATELC in static air-lift culture (ATELC-Static, K3+, P63−, ABCG2−). Accordingly, the cell-cloning efficiency of ATELC-Dynamic (9.72±3.5%) was significantly higher than that of ATELC-Static (2.13±1.46%, p<0.05). The levels of trans-epithelial electrical resistance, light transmittance and areal modulus variation in ATELC-Dynamic all reached those of NLC (p>0.05). Rabbit lamellar keratoplasty showed that the barrier function of ATELC-Dynamic was intact, and there were no signs of epithelial shedding or neovascularization. Furthermore, the ATELC-Dynamic group had similar optical properties and wound healing processes compared with the NLC group. Thus, the sequential dynamic culture process that was designed according to corneal physiological characteristics could successfully reconstruct an auto-lamellar cornea with favorable morphological characteristics and satisfactory physiological function.

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Mechanical Stimuli-induced Urothelial Differentiation in a Human Tissue-engineered Tubular Genitourinary Graft

Cited by 58 publications

References 25 publications

Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice

Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice

Tissue Engineering of Urinary Bladder and Urethra: Advances from Bench to Patients

Reconstruction of Auto-Tissue-Engineered Lamellar Cornea by Dynamic Culture for Transplantation: A Rabbit Model

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