“…Human breast epithelial cells (MCF 10A from ATCC), MCF 10A SS-GFP-KDEL and MCF 10A SS-GFP-SUN1L-KDEL cells (created by Dr. Tanmay Lele and Dr. Kyle Roux [ 21 , 25 ]) were a kind gift to us. The inducible cell lines were created with MCF 10A TET-ON (Clontech) transduced retrovirally with pRetroX-Tight.puro containing either SS-HA-GFP-SUN1L-KDEL for LINC disruption or SS-HA-GFP-KDEL for controls [ 25 ]. To create a stable line, cell selection was performed with 0.5 μg/mL puromycin and then screened by immunofluorescence postinduction with or without 1 μg/mL doxycycline for 18 h. The cells were cultured in DMEM/F12 (Thermofisher, Grand Island, NY, USA) without phenol red, supplemented with 5% ( w/v ) horse serum, cholera toxin (Sigma, Saint Louis, MO USA), epidermal growth factor (Peprotech, Cranbury, NJ, USA), hydrocortisone (Sigma), insulin (Sigma) and penicillin streptomycin (Thermofisher, Grand Island, NY, USA).…”