Mechanical spectroscopy and imaging of skin components in vivo: Assignment of the observed moduli

Silver, Frederick H.; Shah, Ruchit

doi:10.1111/srt.12594

Cited by 13 publications

(57 citation statements)

References 32 publications

(144 reference statements)

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“…Vibrational optical coherence tomography (VOCT) is a new technique developed in the Department of Pathology at Robert Wood Johnson Medical School, Rutgers University. It uses near infrared light and audible sound applied transversely to the tissue surface to image and measure the resonant frequency of tissues . The resonant frequency is directly related to the tissue stiffness measured using conventional uniaxial tensile testing as described previously …”

Section: Methodsmentioning

confidence: 99%

“…OCT cross‐sectional images were obtained using an OQ Labscope (Lumedica Inc) and a laboratory spectral‐domain optical coherence tomography device (SD‐OCT) operating in the scanning mode …”

Section: Methodsmentioning

confidence: 99%

“…Transverse sample displacement was generated by placing a speaker next to the skin or under the excised lesion to be studied by spectral‐domain optical coherence tomography (SD‐OCT), a non‐contact, interferometric technique as discussed previously . The SD‐OCT system uses a fiber‐coupled superluminescent diode light source with an 810 nm center wavelength and 100 nm bandwidth (full‐width at half maximum) as described previously …”

Section: Methodsmentioning

confidence: 99%

“…Identification of tissue margins and tissue stiffness non‐invasively in conjunction with enhanced imaging techniques has the potential to facilitate detection of abnormal skin lesions and lead to early removal. We have developed at technique termed vibrational optical coherence tomography (VOCT) to image skin in three dimensions and measure the stiffness simultaneously, non‐invasively and non‐destructively to provide a “virtual biopsy” of skin and skin lesions …”

Section: Introductionmentioning

confidence: 99%

“…Using VOCT to image and analyze the biomechanical properties of tissues non‐invasively and non‐destructively, it is possible to get a “virtual biopsy” of normal skin and abnormal skin lesions . Correlating these measurements with in vitro calibration data can be used to interpret mechanical measurements made in vivo .…”

Section: Introductionmentioning

confidence: 99%

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Comparative “virtual biopsies” of normal skin and skin lesions using vibrational optical coherence tomography

Silver

Shah

Richard³

et al. 2019

Skin Research and Technology

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132

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Background Increased tissue stiffness (also termed modulus) has been shown to be a characteristic of potential tumor metastasis. Measured values of the stiffness of tumors and cancer cells are reported in the literature to increase compared to neighboring normal tissues. Yet the relationship between the mechanical properties of cells and the extracellular matrix has yet to be correlated with the histopathology of cancerous lesions. Materials and Methods We have developed a technique to do virtual biopsies of skin lesions by combining images made using optical coherence tomography with stiffness measurements made simultaneously using vibrational analysis. The technique is termed vibrational optical coherence tomography (VOCT). Results In this paper, we report that precancerous and cancerous lesions are characterized by changes in both the morphology and stiffness of the cellular components of the skin. The ratio of the peak heights that correspond to the epidermal (40‐60Hz) and dermal (140‐160 Hz) resonant frequencies appear to be different for benign and cancerous or precancerous lesions compared with normal skin and scar. Conclusions Cell‐to‐cell and epidermal‐to‐dermal interactions may be very important in evaluating the potential of skin lesions to become malignant. These interactions can be evaluated using VOCT, a new technique for performing “virtual biopsies” of skin lesions.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative “virtual biopsies” of normal skin and skin lesions using vibrational optical coherence tomography

Silver

Shah

Richard³

et al. 2019

Skin Research and Technology

Self Cite

132

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show abstract

Relaxation time constant based optical coherence elastography

Zhang

Huang

2020

Journal of Biophotonics

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Structural Assessment of Scars Using Optical Techniques

Haasterecht

Zuijlen

Groot

2020

Textbook on Scar Management

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The evaluation of scar treatment benefits from exact structural measurements. Accurate assessment of thickness, surface area, and relief is crucial in routine clinical follow-up. From an experimental perspective, precise visualization of the microstructural organization is necessary for a better understanding of the mechanisms underlying pathological scarring. Structural proteins in scars differ from healthy skin in terms of amount, type, and importantly, organization. The precise quantification of this extracellular matrix (ECM) organization was, until recently, limited to two-dimensional images from fixated and stained tissue. Advances in optical techniques now allow high-resolution imaging of these structures, in some cases in vivo. The enormous potential of these techniques as objective assessment tools is illustrated by a substantial increase in available devices. This chapter describes currently used devices and techniques used in the clinical follow-up of scar progression from a volumetric standpoint. Furthermore, some of the most powerful techniques for microstructural research are described including optical coherence tomography, nonlinear optical techniques such as second harmonic generation microscopy, and confocal microscopy.

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Mechanical spectroscopy and imaging of skin components in vivo: Assignment of the observed moduli

Abstract: Using vibrational OCT, it is possible to identify and map the location of the macromolecular components in skin and skin lesions.

Cited by 13 publications

References 32 publications

Comparative “virtual biopsies” of normal skin and skin lesions using vibrational optical coherence tomography

Comparative “virtual biopsies” of normal skin and skin lesions using vibrational optical coherence tomography

Relaxation time constant based optical coherence elastography

Structural Assessment of Scars Using Optical Techniques

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