Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments

Huang, Xiaolan; Qu, Rongmei; Peng, Yan; Yang, Yuchao; Fan, Tao; Sun, Bing; Khan, Asmat Ullah; Wu, Shutong; Wei, Kuan-hai; Xu, Chujiang; Dai, Jingxing; Ouyang, Jun; Zhang, Shizhen

doi:10.3390/biom11050759

Cited by 4 publications

(4 citation statements)

References 53 publications

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“…The microfilament cytoskeleton formed by actin polymerization plays an important role in osteogenic differentiation of mesenchymal stem cells [ 50 ]. The arrangement of the microfilament cytoskeleton is significantly altered during osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Regulation of the integrin αVβ3- actin filaments axis in early osteogenesis of human fibroblasts under cyclic tensile stress

Peng

Feng

et al. 2021

Stem Cell Res Ther

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Background Integrins play a prominent role in osteogenic differentiation by transmitting both mechanical and chemical signals. Integrin expression is closely associated with tensile stress, which has a positive effect on osteogenic differentiation. We investigated the relationship between integrin αVβ3 and tensile stress. Methods Human fibroblasts were treated with c (RGDyk) and lentivirus transduction to inhibit function of integrin αVβ3. Y-15, cytochalasin D and verteporfin were used to inhibit phosphorylation of FAK, polymerization of microfilament and function of nuclear YAP, respectively. Fibroblasts were exposed to a cyclic tensile stress of 10% at 0.5 Hz, once a day for 2 h each application. Fibroblasts were harvested on day 4 and 7 post-treatment. The expression of ALP, RUNX2, integrin αVβ3, β-actin, talin-1, FAK, vinculin, and nuclear YAP was detected by Western blot or qRT-PCR. The expression and distribution of integrin αVβ3, vinculin, microfilament and nuclear YAP. Results Cyclic tensile stress was found to promote expression of ALP and RUNX2. Inhibition of integrin αVβ3 activation downregulated the rearrangement of microfilament and the expression of ALP, RUNX2 and nuclear YAP. When the polymerization of microfilament was inhibited the expression of ALP, RUNX2 and nuclear YAP were decreased. The phosphorylation of FAK induced by cyclic tensile stress reduced by the inhibition of integrin αVβ3. The expression of ALP and RUNX2 was decreased by inhibition of phosphorylation of FAK and inhibition of nuclear YAP. Conclusions Cyclic tensile stress promotes osteogenesis of human fibroblasts via integrin αVβ3-microfilament axis. Phosphorylation of FAK and nuclear YAP participates in this process.

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Section: Discussionmentioning

confidence: 99%

Regulation of the integrin αVβ3- actin filaments axis in early osteogenesis of human fibroblasts under cyclic tensile stress

Peng

Feng

et al. 2021

Stem Cell Res Ther

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“…1 F) also improved with the prolongation of the osteogenic differentiation time, consistent with findings from our previous work. 5 Immunofluorescence additionally demonstrated that PDLIM5 co-localized with microfilaments ( Fig. S2B, C ).…”

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confidence: 88%

Mechanical sensor PDLIM5 promotes the osteogenesis of human adipose-derived stem cells through microfilament alterations

Yang

et al. 2024

Genes & Diseases

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“…A study on the mechanism of osteogenic differentiation of HSFs found that depolymerization of microfilaments inhibited the expression of osteogenesis-related proteins and ALP activity of HSFs, whereas polymerization of microfilaments enhanced the osteogenic differentiation of HSFs. PDLIM5, a cytoskeleton-associated protein, can mediate osteogenic differentiation of fibroblasts by affecting microfilament formation and polymerization ( 85 ). Another study found that membrane-bound protein A2 promotes the ossification of ligament fibroblasts in patients with ankylosing spondylitis, and experiments suggest that it may function through the extracellular signaling-related kinase pathway ( 86 ).…”

Section: The Mechanism Of Fibroblast Regulating Fracture Healingmentioning

confidence: 99%

Advances in the role and mechanism of fibroblasts in fracture healing

Wang,

Qi,

Shema

et al. 2024

Front. Endocrinol.

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With the development of social population ageing, bone fracture has become a global public health problem due to its high morbidity, disability and mortality. Fracture healing is a complex phenomenon involving the coordinated participation of immigration, differentiation and proliferation of inflammatory cells, angioblasts, fibroblasts, chondroblasts and osteoblasts which synthesize and release bioactive substances of extracellular matrix components, Mortality caused by age-related bone fractures or osteoporosis is steadily increasing worldwide as the population ages. Fibroblasts play an important role in the process of fracture healing. However, it is not clear how the growth factors and extracellular matrix stiffness of the bone-regeneration microenvironment affects the function of osteoblasts and fibroblasts in healing process. Therefore, this article focuses on the role of fibroblasts in the process of fracture healing and mechanisms of research progress.

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Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments

Cited by 4 publications

References 53 publications

Regulation of the integrin αVβ3- actin filaments axis in early osteogenesis of human fibroblasts under cyclic tensile stress

Regulation of the integrin αVβ3- actin filaments axis in early osteogenesis of human fibroblasts under cyclic tensile stress

Mechanical sensor PDLIM5 promotes the osteogenesis of human adipose-derived stem cells through microfilament alterations

Advances in the role and mechanism of fibroblasts in fracture healing

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