Mechanical regulation of vascular network formation in engineered matrices

Lesman, Ayelet; Rosenfeld, Dekel; Landau, Shira; Levenberg, Shulamit

doi:10.1016/j.addr.2015.07.005

Cited by 41 publications

(32 citation statements)

References 66 publications

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“…Remodeling of the vascular network in microdevices is another interesting application. For example, it is known that mechanical stimuli such as shear stress influences the remodeling process (le Noble et al 2004), which has also been confirmed for microdevices (Davies 2003;Lesman et al 2015;Shemesh et al 2015). The resulting effects on the remodeling process have been theoretically studied (Honda & Yoshizato 1997;Pries et al 1998Pries et al , 2001.…”

Section: Pdms Plugmentioning

confidence: 86%

“…These pre‐patterned fluidic channels were used to assay the effect of flow on endothelial physiology (Lesman et al . ; Shemesh et al . ), lymphocyte‐blood vessel interactions (Sato et al .…”

Section: Previous Attempts To Generate a Functional Capillary Meshwormentioning

confidence: 99%

“…2c; Onoe et al 2013). These pre-patterned fluidic channels were used to assay the effect of flow on endothelial physiology (Lesman et al 2015;Shemesh et al 2015), lymphocyte-blood vessel interactions (Sato et al 2015) and the maintenance of liver spheroids (Maher et al 2014).…”

Section: Previous Attempts To Generate a Functional Capillary Meshwormentioning

confidence: 99%

See 2 more Smart Citations

Tissue culture on a chip: Developmental biology applications of self‐organized capillary networks in microfluidic devices

Miura

Yokokawa

2016

Dev Growth Differ

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Organ culture systems are used to elucidate the mechanisms of pattern formation in developmental biology. Various organ culture techniques have been used, but the lack of microcirculation in such cultures impedes the long-term maintenance of larger tissues. Recent advances in microfluidic devices now enable us to utilize selforganized perfusable capillary networks in organ cultures. In this review, we will overview past approaches to organ culture and current technical advances in microfluidic devices, and discuss possible applications of microfluidics towards the study of developmental biology.

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Section: Pdms Plugmentioning

confidence: 86%

Section: Previous Attempts To Generate a Functional Capillary Meshwormentioning

confidence: 99%

See 1 more Smart Citation

Tissue culture on a chip: Developmental biology applications of self‐organized capillary networks in microfluidic devices

Miura

Yokokawa

2016

Dev Growth Differ

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“…This also applies to branching as additional tip cells can emerge from an existing sprout [20]. The sprouts follow gradients of chemotactic factors like VEGF, Semaphorins, and Ephrins as well as physical forces from interstitial flow, strain, and matrix stiffness ( Figure 2B3) [9,11,16,18,[21][22][23][24]. During sprout formation, the inside of these sprouts open up, forming a lumen that can be perfused from the original vessel.…”

Section: Angiogenesismentioning

confidence: 99%

“…The process of anastomosis is guided by the same gradients as in angiogenesis. Additional guidance for connecting both tip cells is obtained via strain gradients [18,24]. This gradient is created by the tip cells pulling on the fibres inside the extra cellular matrix straining these fibres.…”

Section: Anastomosismentioning

confidence: 99%

Recapitulating the Vasculature Using Organ-On-Chip Technology

Pollet

Toonder

2020

Bioengineering

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The development of Vasculature-on-Chip has progressed rapidly over the last decade and recently, a wealth of fabrication possibilities has emerged that can be used for engineering vessels on a chip. All these fabrication methods have their own advantages and disadvantages but, more importantly, the capability of recapitulating the in vivo vasculature differs greatly between them. The first part of this review discusses the biological background of the in vivo vasculature and all the associated processes. We then evaluate the biological relevance of different fabrication methods proposed for Vasculature-on-Chip, we indicate their possibilities and limitations, and we assess which fabrication methods are capable of recapitulating the intrinsic complexity of the vasculature. This review illustrates the complexity involved in developing in vitro vasculature and provides an overview of fabrication methods for Vasculature-on-Chip in relation to the biological relevance of such methods.

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Electrospun Photocrosslinkable Hydrogel Fibrous Scaffolds for Rapid In Vivo Vascularized Skin Flap Regeneration

Sun

Lang

Zhang

et al. 2016

Adv Funct Materials

173

128

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Distal necrosis of random skin flap is always clinical problematic in plastic surgery. The development of 3D functional vascular networks is fundamental for the survival of a local random skin flap. Herein, an effective technique on constructing 3D fibrous scaffolds for accelerated vascularization is demonstrated using a photocrosslinkable natural hydrogel based on gelatin methacryloyl (GelMA) by electrospinning. It is found that the ultraviolet (UV) photocrosslinkable gelatin electrospun hydrogel fibrous membranes exhibit soft adjustable mechanical properties and controllable degradation properties. Furthermore, it is observed that the optimized hydrogel scaffolds can support endothelial cells and dermal fibroblasts adhesion, proliferation, and migration into the scaffolds, which facilitates vascularization. Importantly, a rapid formation of tubes is observed after 3 d seeding of endothelial cells. After GelMA fibrous scaffold implantation below the skin flap in a rat model, it is found that the flap survival rate is higher than the control group, and there is more microvascular formation, which is potentially beneficial for the flap tissue vascularization. These data suggest that GelMA hydrogels can be used for biomedical applications that require the formation of microvascular networks, including the development of complex engineered tissues.

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Mechanical regulation of vascular network formation in engineered matrices

Cited by 41 publications

References 66 publications

Tissue culture on a chip: Developmental biology applications of self‐organized capillary networks in microfluidic devices

Tissue culture on a chip: Developmental biology applications of self‐organized capillary networks in microfluidic devices

Recapitulating the Vasculature Using Organ-On-Chip Technology

Electrospun Photocrosslinkable Hydrogel Fibrous Scaffolds for Rapid In Vivo Vascularized Skin Flap Regeneration

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