Mechanical properties of skinned rabbit psoas and soleus muscle fibres during lengthening: effects of phosphate and Ca2+.

Stienen, G. J. M.; Versteeg, Philip; Papp, Zsombor; Elzinga, G.

doi:10.1113/jphysiol.1992.sp019176

Cited by 74 publications

(104 citation statements)

References 36 publications

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“…Interestingly, the effect of phosphate on force, which is attributed to the shift of cross-bridge population toward a low force-generating state, is accompanied by an increase of the sarcomere elongation needed to reach the break point on the force record during a stretch (21), similarly to the effect of low temperature in our experiments.…”

Section: Discussionsupporting

confidence: 55%

Effect of temperature on cross-bridge properties in intact frog muscle fibers

Colombini

Nocella²,

Benelli³

et al. 2008

American Journal of Physiology-Cell Physiology

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It is well known that the force developed by skeletal muscles increases with temperature. Despite the work done on this subject, the mechanism of force potentiation is still debated. Most of the published papers suggest that force enhancement is due to the increase of the individual cross-bridge force. However, reports on skinned fibers and single-molecule experiments suggest that cross-bridge force is temperature independent. The effects of temperature on cross-bridge properties in intact frog fibers were investigated in this study by applying fast stretches at various tension levels (P) on the tetanus rise at 5°C and 14°C to induce cross-bridge detachment. Cross-bridge number was measured from the force (critical force, Pc) needed to detach the cross-bridge ensemble, and the average cross-bridge strain was calculated from the sarcomere elongation needed to reach Pc (critical length, Lc). Our results show that Pc increased linearly with the force developed at both temperatures, but the Pc /P ratio was considerably smaller at 14°C. This means that the average force per cross bridge is greater at high temperature. This mechanism accounts for all the tetanic force enhancement. The critical length Lc was independent of the tension developed at both temperatures but was significantly lower at high temperature suggesting that cross bridges at 14°C are more strained. The increased cross-bridge strain accounts for the greater average force developed. force enhancement; fast stretches IT IS WELL KNOWN that tetanic tension in skeletal frog and mammalian muscle increases with temperature in the range 0°C to 20°C (5, 8, 9, 16 -19, 23). Most of the data in the literature indicate that the force enhancement is due to an increase of the mean force developed by the individual cross bridge without a significant change in the total number of attached bridges. It has been also reported that increase of cross-bridge force with temperature occurs at the expense of the power stroke extent, which suggests a common mechanism with force development after electrical stimulation (17). A few reports in skinned fibers (14) and single-molecule experiments (15), however, suggest that temperature increases the number of cross bridges without altering the individual cross-bridge force. From the importance of these findings for the understanding of the mechanism of force generation, we have investigated the effect of temperature on cross-bridge properties in single frog muscle fibers by using a technique recently introduced by our group (1) following Flitney and Hirst (7). This technique measures the cross-bridge number from the measurements of the critical tension (P c ) needed to forcibly detach the cross-bridge ensemble by a fast stretch. Mean sarcomere length extension is obtained from measurements of the sarcomere elongation (critical length, L c ) needed to induce the rupture of the cross-bridge ensemble. In contrast to stiffness measurements traditionally used to measure the number of cross bridges, our measurements do not need to assume...

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Section: Discussionsupporting

confidence: 55%

Effect of temperature on cross-bridge properties in intact frog muscle fibers

Colombini

Nocella²,

Benelli³

et al. 2008

American Journal of Physiology-Cell Physiology

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“…In contrast, the difference in tendency to yield between type I and type II motor units (Malamud et al 1996;Stienen et al 1992) did strongly influence the properties of physiologically recruited muscles, at least when comparing muscles of substantially different motor-unit composition. The tendency to yield was significantly greater in SOL than in G, indicating that this property was strongly dependent on fiber type.…”

Section: Intrinsic Mechanical Properties: Tendency To Yieldmentioning

confidence: 89%

“…It has been shown that initial stiffness is less in type II than in type I motor units, suggesting that stiffness should increase less than proportionately with increasing background force (Petit et al 1990). In addition, the extent of yielding has been thought to be less in type II than in type I muscle fibers (Malamud et al 1996;Stienen et al 1992), suggesting that reflex compensation might be less important at higher forces in muscles of mixed fiber type composition. Although reflex compensation for yielding might be less important under these conditions, the stretch reflex would still be expected to reduce the dependence of stiffness on background force.…”

mentioning

confidence: 99%

Intrinsic Properties and Reflex Compensation in Reinnervated Triceps Surae Muscles of the Cat: Effect of Activation Level

Huyghues-Despointes

Cope

Nichols

2003

Journal of Neurophysiology

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Huyghues-Despointes, Clotilde M.J.I., Timothy C. Cope, and T. Richard Nichols. Intrinsic properties and reflex compensation in reinnervated triceps surae muscles of the cat: effect of activation level. J Neurophysiol 90: 1537-1546, 2003. First published May 7, 2003 10.1152/jn.00718.2002. The manner in which activation levels influence intrinsic muscular properties and contributions of the stretch reflex were studied in homogeneous soleus (SOL) and heterogeneous gastrocnemius (G) muscles in the decerebrate cat. Intrinsic mechanical properties were represented by the initial stiffness of the muscle, measured prior to reflex action, and by the tendency of the muscle to yield during stretch in the absence of the stretch reflex. Stiffness regulation by the stretch reflex was evaluated by measuring the extent to which reflex action reduces yielding and the extent to which stiffness depends on background force. Intrinsic mechanical properties were measured in muscles deprived of effective autogenic reflexes using the method of muscular reinnervation. Reinnervated muscles were recruited to force levels comparable to those achieved during natural locomotion. As force declined during crossed-extension reflexes in reinnervated and intact muscles, initial stiffness declined according to similar convex trajectories. The data did not support the hypothesis that, for a given force level, initial stiffness is greatest in populations of predominantly type I motor units. Incremental stiffness (⌬f/⌬l) of both G and SOL increased in the presence of the stretch reflex. Yielding of SOL (ratio of incremental to initial stiffness) substantially decreased in the presence of the stretch reflex over the full range of forces. In reflexive G, yielding significantly decreased for low to intermediate forces, whereas at higher forces, yielding was similar irrespective of the presence or absence of the stretch reflex. The stretch reflex regulates stiffness in both homogeneous and heterogeneous muscles.

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“…(e) Data analysis The transition between the two phases of force increase during stretch was defined by fitting the data with two lines with different slopes (Stienen et al 1992;Getz et al 1998;Pinniger et al 2006;Roots et al 2007). The point at which the lines intersect was considered the force transition point.…”

Section: (B) Solutionsmentioning

confidence: 99%

Pre-power stroke cross bridges contribute to force during stretch of skeletal muscle myofibrils

Rassier

2008

Proc. R. Soc. B.

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When activated skeletal muscle is stretched, force increases in two phases. This study tested the hypothesis that the increase in stretch force during the first phase is produced by pre-power stroke cross bridges. Myofibrils were activated in sarcomere lengths (SLs) between 2.2 and 2.5 mm, and stretched by approximately 5-15 per cent SL. When stretch was performed at 1 mm s K1 SL K1, the transition between the two phases occurred at a critical stretch (SL c ) of 8.4G0.85 nm half-sarcomere (hs)K1 and the force (critical force; F c ) was 1.62G0.24 times the isometric force (nZ23). At stretches performed at a similar velocity (1 mm s K1 SL K1), 2,3-butanedione monoxime (BDM; 1 mM) that biases cross bridges into prepower stroke states decreased the isometric force to 21.45G9.22 per cent, but increased the relative F c to 2.35G0.34 times the isometric force and increased the SL c to 14.6G0.6 nm hs K1 (nZ23), suggesting that pre-power stroke cross bridges are largely responsible for stretch forces.

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Mechanical properties of skinned rabbit psoas and soleus muscle fibres during lengthening: effects of phosphate and Ca2+.

Cited by 74 publications

References 36 publications

Effect of temperature on cross-bridge properties in intact frog muscle fibers

Effect of temperature on cross-bridge properties in intact frog muscle fibers

Intrinsic Properties and Reflex Compensation in Reinnervated Triceps Surae Muscles of the Cat: Effect of Activation Level

Pre-power stroke cross bridges contribute to force during stretch of skeletal muscle myofibrils

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