Mechanical properties of gelatin nanoparticles in dependency of crosslinking time and storage

Weiss, Agnes-Valencia; Fischer, Thorben; Iturri, Jagoba; Benítez, Rafael; Toca-Herrera, José L.; Schneider, Marc

doi:10.1016/j.colsurfb.2018.12.005

Cited by 35 publications

(26 citation statements)

References 47 publications

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“…As can be seen, the mean size of GNPs determined by SEM micrograph is lower than that of DLS analysis. Presumably, this is due to drying of samples before SEM imaging in contrast to the hydrated particles in aqueous dispersion …”

Section: Resultsmentioning

confidence: 99%

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Design and Characterization of Surface‐Crosslinked Gelatin Nanoparticles for the Delivery of Hydrophilic Macromolecular Drugs

Baseer

Koenneke

Zapp

et al. 2019

Macro Chemistry & Physics

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For nanotechnology enabled delivery of hydrophilic protein‐based drugs, several polymer‐based carrier systems have been used in the past to protect the sensitive load and to facilitate cellular uptake and crossing of biological barriers. This study uses gelatin, a natural and biodegradable macromolecule, as carrier material which is approved for several applications. Nanoprecipitation is used to form nanoparticles and to maintain the physicochemical integrity of gelatin, hydrophilic crosslinkers, e.g., paraformaldehyde, glutaraldehyde, carbodiimide, and transglutaminase are employed. However, these crosslinkers diffuse homogenously into the carrier matrix also crosslinking the polymeric matrix with the entrapped protein‐based molecules thus rendering it inactive. Hence a hydrophobic zero‐length crosslinker, diisopropylcarbodiimide, is applied to avoid diffusion into the particles. This will provide an opportunity to encapsulate protein‐based drugs in the non‐crosslinked matrix. The hypothesis of surface crosslinking is proven by the extent of crosslinking and more importantly by encapsulation and the release of lysozyme as a model hydrophilic protein. Furthermore, essential process parameters are evaluated such as crosslinker concentration, crosslinking time and crosslinking reaction temperature with regard to the effect on particle size, size distribution and zeta‐potential of gelatin nanoparticles. The optimum formulation results in the production of gelatin nanoparticles with 200‐300 nm and a polydispersity index < 0.2.

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Section: Resultsmentioning

confidence: 99%

“…Presumably, this is due to drying of samples before SEM imaging in contrast to the hydrated particles in aqueous dispersion. [42,51]…”

Section: Morphological Characterization-scanning Electron Microscopymentioning

confidence: 99%

Design and Characterization of Surface‐Crosslinked Gelatin Nanoparticles for the Delivery of Hydrophilic Macromolecular Drugs

Baseer

Koenneke

Zapp

et al. 2019

Macro Chemistry & Physics

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“…One way to improve the latter is to conduct a physical, chemical, or enzymatic crosslinking process. Improvement of the mechanical properties of materials after a crosslinking process is often observed [ 26 , 27 , 28 ]. Crosslinking provides a more complex structure of the polymer chain and additional bonds.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the use of a crosslinking agent (glyoxal), no statistically significant differences were observed in the comprehensive modulus, the maximum tension, and the percentage of deformation at the maximum tension of chitosan hydrogels after modification with different concentrations of tannic acid [ 29 ]. Additionally, hydrogel gelatin nanoparticles crosslinked at different times with glutaraldehyde had no influence on the Young’s modulus values [ 27 ]. However, Ahammed et al (2021) observed improved mechanical properties of gelatin/zein films crosslinked with transglutaminase that were proportional to the crosslinking ratio [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Collagen/Beta Glucan Hydrogels Crosslinked with Tannic Acid

Michalska‐Sionkowska

Warżyńska

Kaczmarek-Szczepańska

et al. 2021

Polymers

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Hydrogels based on collagen/β-glucan crosslinked with tannic acid were obtained by neutralization using dialysis. The presence of tannic acid allowed obtaining stable hydrogel materials with better mechanical properties. Tannic acid was released from matrices gradually and not rapidly. The antioxidant properties of the obtained hydrogels increased over the course of their incubation in culture media and were dependent on the concentration of tannic acid in the matrices. The obtained materials influenced dehydrogenase activity and the ATP level of pathogens. Additionally, the materials’ extracts improved the HaCaT cells’ viability. Therefore, the obtained hydrogels seem to be promising biocompatible materials which display antimicrobial properties.

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“…An incompressible Neo-Hookean solid model was chosen for the TEVG and cardiac muscle so as to better predict its nonlinear stress-strain behavior. The Poisson’s ratio (υ) of the TEVG and cardiac muscle was set to be 0.45 [32,33]. As for the Lamé’s coefficients of the neo-Hookean hyperelastic materials, the Lamé’s second coefficients (μ) of the TEVG and muscle were set to be 6.20 × 10 6 N/m 2 and 7.20 × 10 6 N/m 2 , respectively.…”

Section: Methodsmentioning

confidence: 99%

A Novel Biodegradable Multilayered Bioengineered Vascular Construct with a Curved Structure and Multi-Branches

et al. 2019

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Constructing tissue engineered vascular grafts (TEVG) is of great significance for cardiovascular research. However, most of the fabrication techniques are unable to construct TEVG with a bifurcated and curved structure. This paper presents multilayered biodegradable TEVGs with a curved structure and multi-branches. The technique combined 3D printed molds and casting hydrogel and sacrificial material to create vessel-mimicking constructs with customizable structural parameters. Compared with other fabrication methods, the proposed technique can create more native-like 3D geometries. The diameter and wall thickness of the fabricated constructs can be independently controlled, providing a feasible approach for TEVG construction. Enzymatically-crosslinked gelatin was used as the material of the constructs. The mechanical properties and thermostability of the constructs were evaluated. Fluid-structure interaction simulations were conducted to examine the displacement of the construct’s wall when blood flows through it. Human umbilical vein endothelial cells (HUVECs) were seeded on the inner channel of the constructs and cultured for 72 h. The cell morphology was assessed. The results showed that the proposed technique had good application potentials, and will hopefully provide a novel technological approach for constructing integrated vasculature for tissue engineering.

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Mechanical properties of gelatin nanoparticles in dependency of crosslinking time and storage

Cited by 35 publications

References 47 publications

Design and Characterization of Surface‐Crosslinked Gelatin Nanoparticles for the Delivery of Hydrophilic Macromolecular Drugs

Design and Characterization of Surface‐Crosslinked Gelatin Nanoparticles for the Delivery of Hydrophilic Macromolecular Drugs

Characterization of Collagen/Beta Glucan Hydrogels Crosslinked with Tannic Acid

A Novel Biodegradable Multilayered Bioengineered Vascular Construct with a Curved Structure and Multi-Branches

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