Mechanical properties and permeability of porous chitosan–poly(p-dioxanone)/silk fibroin conduits used for peripheral nerve repair

Wu, Hua; Zhang, Jun; Luo, Ying; Wan, Ying; Sun, Shi‐Gang

doi:10.1016/j.jmbbm.2015.06.016

Cited by 22 publications

(11 citation statements)

References 53 publications

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“…For peripheral nerve regeneration, the conduits could provide an adequate scaffold for cell adhesion and axonal regeneration, and could be semi-permeable for the metabolic exchange (such as oxygen and waste products)11. In addition, the wall of the conduit usually can slow down the diffusion of the growth or trophic factors secreted by the nerve stumps12. A wide range of natural and synthetic materials have been developed for nerve conduits, such as PLA13, Chitosan14, and Gelatin15.…”

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confidence: 99%

A 3D-engineered porous conduit for peripheral nerve repair

Tao

Wang

et al. 2017

Sci Rep

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End-to-end neurorrhaphy is the most commonly used method for treating peripheral nerve injury. However, only 50% of patients can regain useful function after treating with neurorrhaphy. Here, we constructed a 3D-engineered porous conduit to promote the function recovery of the transected peripheral nerve after neurorrhaphy. The conduit that consisted of a gelatin cryogel was prepared by molding with 3D-printed moulds. Due to its porous structure and excellent mechanical properties, this conduit could be collapsed by the mechanical force and resumed its original shape after absorption of normal saline. This shape-memory property allowed a simply surgery process for installing the conduits. Moreover, the biodegradable conduit could prevent the infiltration of fibroblasts and reduce the risk of scar tissue, which could provide an advantageous environment for nerve regeneration. The efficiency of the conduits in assisting peripheral nerve regeneration after neurorrhaphy was evaluated in a rat sciatic nerve transected model. Results indicated that conduits significantly benefitted the recovery of the transected peripheral nerve after end-to-end neurorrhaphy on the static sciatic index (SSI), electrophysiological results and the re-innervation of the gastrocnemius muscle. This work demonstrates a biodegradable nerve conduit that has potentially clinical application in promoting the neurorrhaphy.

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confidence: 99%

A 3D-engineered porous conduit for peripheral nerve repair

Tao

Wang

et al. 2017

Sci Rep

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“…Figure S1 provides FTIR spectra for chitosan, PDO and CH-PDO; and a representative 1 H NMR spectrum for the obtained CH-PDO. It confirms that the PDO side chains have been successfully conjugated onto the CH backbone [13,22]. Among the obtained CH-PDOs, the CH-PDO with a PDO content of about 30 wt% was selected for the follow-up preparation of complex NPs, taking into consideration the solubility of the CH-PDO in the 1.0% aqueous acetic acid solution.…”

Section: Resultsmentioning

confidence: 85%

“…It can be nearly completely depolymerized under conditions of reduced pressure and suitable temperatures, enabling it to be an environment-friendly polymer [21,22]. By grafting PDO oligomers onto chitosan units, the resulting CH-PDO could be soluble in aqueous media while showing the merits of both CH and PDO [13]. In the present study, a series of CH-PDOs with varied substitution degrees of PDO was synthesized by grafting PDO onto the C-6 sites of CH backbone while leaving the amino groups at the C-2 sites of CH units free for subsequent crosslinking.…”

Section: Resultsmentioning

confidence: 99%

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Chitosan-Based Hydrogels Embedded with Hyaluronic Acid Complex Nanoparticles for Controlled Delivery of Bone Morphogenetic Protein-2

Liu

et al. 2019

Pharmaceutics

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Chitosan(CH)-poly(dioxanone) (CH-PDO) copolymers containing varied amounts of PDO and having free amino groups at their CH backbone were synthesized using a group protection method. The selected CH-PDO with soluble characteristics in aqueous media was used together with hyaluronic acid (HA) to prepare HA/CH-PDO polyelectrolyte complex nanoparticles (NPs) via an ionotropic gelation technique, and such a type of HA/CH-PDO NPs was employed as a carrier for delivering bone morphogenetic protein-2 (BMP-2). The optimal BMP-2-encapsulated HA/CH-PDO NPs with high encapsulation efficiency were embedded into CH/glycerophosphate composite solutions to form different hydrogels in order to achieve long-term BMP-2 release. The formulated gels were found to be injectable at room temperature and had its thermosensitive phase transition near physiological temperature and pH. They also showed abilities to administer the release of BMP-2 in approximately linear manners for a few weeks while effectively preserving the bioactivity of the encapsulated BMP-2. In view of their fully biocompatible and biodegradable components, the presently developed gel systems have promising potential for translation to the clinic use in bone repair and regeneration where the sustained and controlled stimuli from active signaling molecules and the stable biomechanical framework for housing the recruited cells are often concurrently needed.

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“…Reduced to their most basic morphological features, nerves and nerve guides or conduits are tubular structures and several leading fabrication approaches have been used to create scaffolds that conform to this general form [15][16][17]. Wet-spinning is a well-established, versatile fabrication technique that has been used to produce long homogenous bio-fibres with diameters ranging from nanometres to millimetres [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Cell compatible encapsulation of filaments into 3D hydrogels

Schirmer¹,

Gorkin²,

Beirne³

et al. 2016

Biofabrication

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Tissue engineering scaffolds for nerve regeneration, or artificial nerve conduits, are particularly challenging due to the high level of complexity the structure of the nerve presents. The list of requirements for artificial nerve conduits is long and includes the ability to physically guide nerve growth using physical and chemical cues as well as electrical stimulation. Combining these characteristics into a conduit, while maintaining biocompatibility and biodegradability, has not been satisfactorily achieved by currently employed fabrication techniques. Here we present a method combining pultrusion and wet-spinning techniques facilitating incorporation of pre-formed filaments into ionically crosslinkable hydrogels. This new biofabrication technique allows the incorporation of conducting or drug-laden filaments, controlled guidance channels and living cells into hydrogels, creating new improved conduit designs.

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Mechanical properties and permeability of porous chitosan–poly(p-dioxanone)/silk fibroin conduits used for peripheral nerve repair

Cited by 22 publications

References 53 publications

A 3D-engineered porous conduit for peripheral nerve repair

A 3D-engineered porous conduit for peripheral nerve repair

Chitosan-Based Hydrogels Embedded with Hyaluronic Acid Complex Nanoparticles for Controlled Delivery of Bone Morphogenetic Protein-2

Cell compatible encapsulation of filaments into 3D hydrogels

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