Mechanical Properties and Hydroxyproline Content of Connective Tissue in Porous Ceramic Implants

Abstract

I . Mechanical properties and hydroxyproline content of connective tissue in porous ceramic implants. Acta path. microbiol. sand. Sect. A. 87: 193-200. 1979.'The present study describes a model applying ceramic implants (A 1203) for iri i ' i i~ studies of connective tissue regeneration. Two types of implants have been developed: a one-piece model for histological examination and chemical analyses. and a two-piece implant which can also be used for mechanical testing of connective tissue. When these were implanted subcutaneously on the back of rats. a correlation was found between the mechanical strength and the hydroxyproline content of connective tissue in the implants. The peak synthesis occurred between the 7th and the 14th day after implantation, and a plateau was reached for both strength-increases and hydroxyproline-formation between the 14th and the 2lst days. For histological examination. the implants were embedded in plastic materials and prepared as hard tissue specimens. The model presented can be applied to study connective tissue regeneration in normal and pathological conditions. including studies of the effects of various drugs on connective tissue.

Effects of Cyclophosphamide on the Formation and Solubility of Collagen

“…Incisional skin wounds were inflicted under aseptic conditions using the procedure described by Wie et at. (1979b), and porous ceramic implants were inserted subcutaneously on the left side of the animals (Wie & Beck 1979). Twenty-four hours prior to sacrifice, the animals received 15 pCi/IOO g body weight L-(U-l4C) proline (The Radiochemical Centre, Amersham, Bucks, England, sp act.…”

“…Dehydration to constant weight was accomplished in an oven at 35" for 3 days. After drying, the implants were ground down to a powder and subjected to fractionation procedures as described by Wie & Beck (1979). Soluble collagen was extracted in 0.5 M NaCl at 4" for 20 hours in a shaking machine.…”