Mechanical properties and biocompatibility of two polyepoxy matrices: DGEBA-DDM and DGEBA-IPD

Berruet, R.; Vinard, E.; Calle, A. de la; Tighzert, H.L.; Chabert, B.; Magloire, H.; Eloy, R.

doi:10.1016/0142-9612(87)90058-5

Cited by 16 publications

(15 citation statements)

References 5 publications

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“…Particularly, 4 d after challenge, these bacteria were co-localised with macrophages, suggesting that the macrophages were not effectively killing the bacteria. This phenomenon has been observed in many earlier studies (Boelens et al, 2000a;Boelens et al, 2000b;. However, from the tissue around the CHX 10 -coated implants, only few bacteria were cultured and few bacteria were seen by histology.…”

Section: Discussionmentioning

confidence: 74%

“…The tissue samples were homogenised in 0.5 mL of PBS using 5 zirconia beads (2 mm diameter, BioSpec Products, Bartlesville, OK, USA) and the MagnaLyser System (Roche), with 3 cycles of 30 s at 7000 rpm, with 30 s cooling on ice between cycles. Like the sonication procedure of the implants, this homogenisation procedure does not affect the viability of staphylococci (Boelens et al, 2000a). The sonicates and homogenates were 10-fold serially diluted and plated on blood agar plates.…”

Section: Mouse Experimental Biomaterial-associated Infection Modelmentioning

confidence: 99%

“…After incubation, each disk and the corresponding filter were placed together in 5 mL of TSB, sonicated for 30 s in a sonicator water bath (Elma Transsonic T460, 35 kHz; Elma Schmidbauer GmbH, Singen, Germany) and vortexed for 1 min to dislodge adherent bacteria. This procedure does not affect bacterial viability (Boelens et al, 2000a). Per sample, two independent 10-fold serial dilutions were made in a microtiter plate and duplicate 10 µL aliquots of the undiluted suspension and of the dilutions were pipetted onto blood agar plates.…”

Section: Surface Bactericidal Activity Assaymentioning

confidence: 99%

“…Standardised biopsies (diameter 12 mm) were taken from the implantation sites as described previously (Boelens et al, 2000a;Riool et al, 2014) and subsequently mice were euthanised by cervical dislocation. Each biopsy included skin, subcutaneous tissue and the implant.…”

Section: Mouse Experimental Biomaterial-associated Infection Modelmentioning

confidence: 99%

“…Coatings and materials manufactured from epoxy resins are among the few available that meet this requirement (Berruet et al, 1987;Garcia et al, 2009). Epoxy resins can be catalytically cross-linked or they can be cured using reagents such as polyamines.…”

Section: Introductionmentioning

confidence: 99%

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A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection

Riool,

Dirks,

Jaspers

et al. 2107

eCM

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Prevention of biomaterial-associated infections (BAI) remains a challenging problem, in particular due to the increased risk of resistance development with the current antibiotic-based strategies. Metallic orthopaedic devices, such as non-cemented implants, are often inserted under high mechanical stress. These non-cemented implants cannot be protected by e.g. antibiotic-releasing bone cement or other antimicrobial approaches, such as the use of bioactive glass. Therefore, in order to avoid abrasion during implantation procedures, we developed an antimicrobial coating with great mechanical stability for orthopaedic implants, to prevent Staphylococcus aureus BAI. We incorporated 5 and 10 wt % chlorhexidine in a novel mechanically stable epoxy-based coating, designated CHX 5 and CHX 10 , respectively. The coatings displayed potent bactericidal activity in vitro against S. aureus, with over 80 % of the release (19 µg/cm 2 for CHX 5 and 41 µg/ cm 2 for CHX 10 ) occurring within the first 24 h. In mice, the CHX 10 coating significantly reduced the number of CFU (colony forming units), both on the implants and in the peri-implant tissues, 1 d after S. aureus challenge. The CHX 10 -coated implants were well-tolerated by the animals, with no signs of toxicity observed by histological analysis. Moreover, the coating significantly reduced the frequency of culture-positive tissues 1 d, and of culturepositive implants 1 and 4 d after challenge. In summary, the chlorhexidine-releasing mechanically stable epoxybased CHX 10 coating prevented implant colonisation and S. aureus BAI in mice and has good prospects for clinical development.

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Section: Discussionmentioning

confidence: 74%

Section: Mouse Experimental Biomaterial-associated Infection Modelmentioning

confidence: 99%

Section: Surface Bactericidal Activity Assaymentioning

confidence: 99%

Section: Mouse Experimental Biomaterial-associated Infection Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection

Riool,

Dirks,

Jaspers

et al. 2107

eCM

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Epoxy networks for medicine applications: Mechanical properties and in vitro biological properties

García

Leyva

Queiroz

et al. 2009

J of Applied Polymer Sci

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This work describes the physicochemical, mechanical, and in vitro biological properties of three epoxy networks based on diglycidyl ether of bisphenol-A (DGEBA) epoxy prepolymer cured with triethylenetetramine, 1-(2-aminoethyl)piperazine (AEP) and isophoronediamine. The mechanical properties were evaluated with respect to impact and flexural tests. Functionality rules the mechanical behavior of epoxy networks by increasing the crosslink density and the flexural modulus, increasing T g and decreasing the chain flexibility and the impact resistance. The biological interactions between the obtained epoxy polymers and blood were studied by in vitro methods. Studies on the protein adsorption, platelet adhesion, and thrombus formation are presented. The protein adsorption assays onto polymeric surfaces showed that the epoxy networks adsorbed more albumin than fibrinogen. The results about platelet adhesion and thrombus formation indicated that DGEBA-IPD and DGEBA-AEP networks exhibits good hemocompatible behavior. The materials revealed no signs of cytotoxicity to Chinese hamster ovary cells, showing a satisfactory cytocompatibility. In this way, we can assume that the epoxy polymers are biocompatible materials.

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A Minimally Toxic Dose of Methylene Dianiline Injures Biliary Epithelial Cells in Rats

Kaphalia

et al. 1998

Toxicology and Applied Pharmacology

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Mechanical properties and biocompatibility of two polyepoxy matrices: DGEBA-DDM and DGEBA-IPD

Cited by 16 publications

References 5 publications

A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection

A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection

Epoxy networks for medicine applications: Mechanical properties and in vitro biological properties

A Minimally Toxic Dose of Methylene Dianiline Injures Biliary Epithelial Cells in Rats

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