Mechanical oscillation of dynamic microtubule rings

Ito, Masaki; Kabir, Arif Md. Rashedul; Islam, Md. Sirajul; Inoue, Daisuke; Wada, Shoki; Sada, Kazuki; Konagaya, Akihiko; Kakugo, Akira

doi:10.1039/c6ra16613j

Cited by 6 publications

(3 citation statements)

References 34 publications

(34 reference statements)

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“…100 The initial descriptions of the nanowire and nanospool structures formed when microtubules gliding on a surface coated with kinesin motors can interact with each other via biotin-streptavidin linkages 80, 81 have been complemented by detailed studies elucidating the assembly process. 101–116 Under suitable conditions, microtubule bundles with close to millimeter dimensions have been obtained, and a mathematical description of the process captured the fundamental differences between diffusive and active transport of building blocks in self-assembly (Fig. 6a).…”

Section: Non-equilibrium Self-assembly and Self-organization Of Micromentioning

confidence: 99%

Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots

2017

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Biological systems have evolved to harness non-equilibrium processes from the molecular to the macro scale. It is currently a grand challenge of chemistry, materials science, and engineering to understand and mimic biological systems that have the ability to autonomously sense stimuli, process these inputs, and respond by performing mechanical work. New chemical systems are responding to the challenge and form the basis for future responsive, adaptive, and active materials. In this article, we describe a particular biochemical-biomechanical network based on the microtubule cytoskeletal filament – itself a non-equilibrium chemical system. We trace the non-equilibrium aspects of the system from molecules to networks and describe how the cell uses this system to perform active work in essential processes. Finally, we discuss how microtubule-based engineered systems can serve as testbeds for autonomous chemical robots composed of biological and synthetic components.

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Section: Non-equilibrium Self-assembly and Self-organization Of Micromentioning

confidence: 99%

Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots

2017

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“…Applying methylcellulose in the solution, weak interactions between the MT filaments can be induced from the resultant depletion forces, depending on the concentration of the depletant agent and the density of filaments (Inoue et al 2015;Saito et al 2017). A wide range of phase transitions from single filaments to different swarm patterns was obtained by strong interactions between filaments, induced by crosslinking of actin filaments by fascin (Takatsuki et al 2014), whereas biotinylated MTs by streptavidin (Bachand et al 2005; lead to the formation of long-lived patterns (Idan et al 2011;Luria et al 2011;Lam et al 2014;Wada et al 2014Wada et al , 2015Ito et al 2016;VanDelinder et al 2016;Martinez et al 2019). By using this method, a wide variety of assembled structures was obtained, e.g., bundle, network, and ring-shaped structures, that differs in size or shape (Kawamura et al 2008(Kawamura et al , 2009(Kawamura et al , 2010Liu and Bachand 2013).…”

Section: Synchronization Of Biomolecular Motor-driven Filaments Into mentioning

confidence: 99%

Synchronous operation of biomolecular engines

2020

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Biomolecular motor systems are the smallest natural machines with an ability to convert chemical energy into mechanical work with remarkably high efficiency. Such attractive features enabled biomolecular motors to become classic tools in soft matter research over the past decade. For designing suitably engineered biomimetic systems, the biomolecular motors can potentially be used as molecular engines that can transform energy and ensure great advantages for the construction of bio-nanodevices and molecular robots. From the optimization of their prolonged lifetime to coordinate them into highly complex and ordered structures, enormous efforts have been devoted to make them useful in the synthetic environment. Synchronous operation of the biomolecular engines is one of the key criteria to coordinate them into certain different patterns, which depends on the local interaction of biomolecular motors. Utilizing chemical and physical stimuli, synchronization of biomolecular motor systems has become possible, which allows them to coordinate into different higher ordered patterns with different modes of functionality. Recently, programmed synchronous operation of the biomolecular engines has also been demonstrated, using a smart biomaterial to build up swarms reminiscent of nature. Here, we review the recent progress in the synchronized operation of biomolecular motors in engineered systems to explicitly program their interaction and further their applications. Such developments in the coordination of biomolecular motors have opened a broad way to explore the construction of future autonomous molecular machines and robots based on synchronization of biomolecular engines.

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“…Furthermore, interactions with the boundaries affect the system . Strong interactions between filaments, induced, e.g., by cross-linking of biotinylated microtubules by streptavidin , or actin filaments by fascin, lead to the formation of long-lived wire-like and ring-like structures. ,,− Weak interactions between filaments can result for example from depletion forces, where macromolecules such as methylcellulose added to the solution induce bundling in a concentration dependent manner (Figure ). , …”

Section: Collective Effects and Their Applicationsmentioning

confidence: 99%

Synthetic Systems Powered by Biological Molecular Motors

2019

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Biological molecular motors (or biomolecular motors for short) are nature’s solution to the efficient conversion of chemical energy to mechanical movement. In biological systems, these fascinating molecules are responsible for movement of molecules, organelles, cells, and whole animals. In engineered systems, these motors can potentially be used to power actuators and engines, shuttle cargo to sensors, and enable new computing paradigms. Here, we review the progress in the past decade in the integration of biomolecular motors into hybrid nanosystems. After briefly introducing the motor proteins kinesin and myosin and their associated cytoskeletal filaments, we review recent work aiming for the integration of these biomolecular motors into actuators, sensors, and computing devices. In some systems, the creation of mechanical work and the processing of information become intertwined at the molecular scale, creating a fascinating type of “active matter”. We discuss efforts to optimize biomolecular motor performance, construct new motors combining artificial and biological components, and contrast biomolecular motors with current artificial molecular motors. A recurrent theme in the work of the past decade was the induction and utilization of collective behavior between motile systems powered by biomolecular motors, and we discuss these advances. The exertion of external control over the motile structures powered by biomolecular motors has remained a topic of many studies describing exciting progress. Finally, we review the current limitations and challenges for the construction of hybrid systems powered by biomolecular motors and try to ascertain if there are theoretical performance limits. Engineering with biomolecular motors has the potential to yield commercially viable devices, but it also sharpens our understanding of the design problems solved by evolution in nature. This increased understanding is valuable for synthetic biology and potentially also for medicine.

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Mechanical oscillation of dynamic microtubule rings

Cited by 6 publications

References 34 publications

Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots

Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots

Synchronous operation of biomolecular engines

Synthetic Systems Powered by Biological Molecular Motors

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