Mechanical Nanosensor Based on FRET within a Thermosome: Damage‐Reporting Polymeric Materials

Bruns, Nico; Pustelny, Katarzyna; Bergeron, Lisa M.; Whitehead, Timothy A.; Clark, Douglas S.

doi:10.1002/ange.200900554

Cited by 36 publications

(23 citation statements)

References 29 publications

(15 reference statements)

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“…Time-domain fluorescence lifetime imaging microscopy (FLIM) for additional FRET sensor characterization was implemented with an inverted Zeiss LSM510 Axiovert 200M microscope with a Plan NeoFLUAR 40×/1.3 NA DIC oil-immersion objective lens, equipped with a TCSPC controller (SPC-830 card; Becker & Hickl, Berlin, Germany) as described previously 32 . CFP was excited with 440 nm light generated by frequency doubling of 880 nm pulses from a mode-locked Ti:sapphire laser(Mai-Tai, Spectra-Physics, 120–150 fs pulse width, 80 MHz repetition rate, and Frequency Doubler and Pulse Selector, Spectra-Physics, Model 3980).…”

Section: Methodsmentioning

confidence: 99%

The cancer glycocalyx mechanically primes integrin-mediated growth and survival

Paszek¹,

DuFort²,

Rossier

et al. 2014

Nature

609

646

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Malignancy is associated with altered expression of glycans and glycoproteins that contribute to the cellular glycocalyx. We constructed a glycoprotein expression signature, which revealed that metastatic tumours upregulate expression of bulky glycoproteins. A computational model predicted that these glycoproteins would influence transmembrane receptor spatial organization and function. We tested this prediction by investigating whether bulky glycoproteins in the glycocalyx promote a tumour phenotype in human cells by increasing integrin adhesion and signalling. Our data revealed that a bulky glycocalyx facilitates integrin clustering by funnelling active integrins into adhesions and altering integrin state by applying tension to matrix-bound integrins, independent of actomyosin contractility. Expression of large tumour-associated glycoproteins in non-transformed mammary cells promoted focal adhesion assembly and facilitated integrin-dependent growth factor signalling to support cell growth and survival. Clinical studies revealed that large glycoproteins are abundantly expressed on circulating tumour cells from patients with advanced disease. Thus, a bulky glycocalyx is a feature of tumour cells that could foster metastasis by mechanically enhancing cell-surface receptor function.

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Section: Methodsmentioning

confidence: 99%

The cancer glycocalyx mechanically primes integrin-mediated growth and survival

Paszek¹,

DuFort²,

Rossier

et al. 2014

Nature

609

646

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“…Using two complementary hetero-bifunctional linkers confers the further advantage that cross-linking cannot occur. Such a system was used to specifically and quantitatively conjugate oligonucleotides to antibodies [63] and to bind proteins to other proteins [64] in aqueous solution. Because this conjugation reaction is carried out under mild conditions in aqueous buffer, without any additional catalyzing agents (e.g., in contrast to the copper catalyzed azide-alkyne click reaction), the resulting conjugates are applicable for pharmaceutical and therapeutic purposes.…”

Section: Aminementioning

confidence: 99%

Functionalization of Block Copolymer Vesicle Surfaces

et al. 2011

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Abstract:In dilute aqueous solutions certain amphiphilic block copolymers self-assemble into vesicles that enclose a small pool of water with a membrane. Such polymersomes have promising applications ranging from targeted drug-delivery devices, to biosensors, and nanoreactors. Interactions between block copolymer membranes and their surroundings are important factors that determine their potential biomedical applications. Such interactions are influenced predominantly by the membrane surface. We review methods to functionalize block copolymer vesicle surfaces by chemical means with ligands such as antibodies, adhesion moieties, enzymes, carbohydrates and fluorophores. Furthermore, surface-functionalization can be achieved by self-assembly of polymers that carry ligands at their chain ends or in their hydrophilic blocks. While this review focuses on the strategies to functionalize vesicle surfaces, the applications realized by, and envisioned for, such functional polymersomes are also highlighted.

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“…[1][2][3][4] Mechanochromic polymeric materials, which change color under an applied force, have been well studied. [5][6][7][8][9] Spiropyran (SP) is one of the most promising mechanophores, which is colorless and undergoes a 6-π electrocyclic ring-opening reaction to form colored merocyanine (MC) under external force. 10,11 SP based mechanochromic materials can be obtained by covalent [10][11][12][13][14][15] and non-covalent bonding to the matrix.…”

Section: Introductionmentioning

confidence: 99%

Color-Switchable Polar Polymeric Materials

Qiu

Gurr

Qiao

2019

ACS Appl. Mater. Interfaces

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Spiropyran is an important mechanophore which has rarely been incorporated as a crosslinker in polar polymer matrices, limiting its applications in innovative mechanochromic devices. Here, three spiropyrans with two or three-attachment positions were synthesized and covalently bonded in polar poly(hydroxyethyl acrylate) (PHEA), to achieve color switchable materials, triggered by light and when swollen in water. The negative photochromism in the dark and mechanical activation by swelling in water were investigated. Measurements of negative photochromism were conducted in solution and crosslinked PHEA bulk polymers, with both showing color reversibility when stored in the dark or exposure to visible light. The force of swelling in water was sufficient to induce the ring-opening reaction of spiropyran polymers. It was found that tri-substituted spiropyran (SP3) was less influenced by the polar matrix but showed the fastest color activation during swelling. SP3 also showed accelerated ring opening to the colored state during the swelling process. Bleaching rates and color switchability were investigated under swollen and dehydrated conditions. The effect of cross-link density on the swelling activation was explored to better understand the interaction between the mechanophore and the polar environment. The results demonstrated that influences from both the polar environment and the mechanochromic nature of spiropyran had an impact on the absorption intensity, rate of change and the decoloration rate of the materials. This study

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Mechanical Nanosensor Based on FRET within a Thermosome: Damage‐Reporting Polymeric Materials

Cited by 36 publications

References 29 publications

The cancer glycocalyx mechanically primes integrin-mediated growth and survival

The cancer glycocalyx mechanically primes integrin-mediated growth and survival

Functionalization of Block Copolymer Vesicle Surfaces

Color-Switchable Polar Polymeric Materials

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