Mechanical micronization of lipoaspirates for the treatment of hypertrophic scars

Wang, Jing; Liao, Yunjun; Xia, Jing; Wang, Zijue; Mo, Xiaopei; Feng, Jiaxuan; He, Yunfan; Chen, Xihang; Li, Ye; Lu, Feng; Cai, Junrong

doi:10.1186/s13287-019-1140-1

Cited by 46 publications

(36 citation statements)

References 29 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have demonstrated the capacity of ADSCs to promote phenotype switching in macrophages, inhibiting expression of M1 polarization genes. Consistent with previous reports [28], SVF gel decreased macrophage in ltration and pro-in ammatory cytokines, indicative of decreased in ammation. Further, apoptotic adipose cells and skin lesion brosis were decreased in mice treated with SVF gel.…”

Section: Discussionsupporting

confidence: 92%

“…Lipotransfer has already been explored as a therapeutic approach for reversing brosis in a number of brotic conditions [28]. In 2012, Ould-ali et al demonstrated that implantation of human adipose tissue signi cantly improved brosis in sclerotic skin lesions of the BLM-induced mouse model, suggesting autologous adipose tissue as a promising novel therapy for scleroderma [29].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanical micronization of lipoaspirates for treatment of bleomycin-induced scleroderma in nude mice

Jiang

Wang

Lin

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background Scleroderma is a chronic autoimmune disease that causes hardening of the skin. Adipose tissue is used as a regenerative treatment for scleroderma, and mechanical micronization of adipose tissue can concentrate stem cells, improving its therapeutic efficacy. Stromal vascular fraction gel (SVF gel) is produced by subjecting lipoaspirates to a series of mechanical processes. The present study aimed to assess the therapeutic effect of SVF gel on dermal scleroderma. Methods Scleroderma was induced in nude mice by daily subcutaneous injections of bleomycin (BLM) for 4 weeks. SVF gel, cell-enriched lipoaspirates (CAL), or Coleman fat (CF) (0.1 mL) was injected subcutaneously into local sclerosis lesions. Histological analyses, TUNEL assay, and qRT-PCR were conducted 4 weeks post-transplantation. Results SVF gel, CAL, and CV decreased dermal thickness in the scleroderma model (P < 0.05), and sclerosis was significantly decreased in the SVF gel and CAL groups. SVF gel and CAL decreased macrophage infiltration, pro-inflammatory cytokine levels, and fibrosis in transferred fat and/or skin lesions (P < 0.05). This revealed anti-inflammatory and anti-fibrotic effects of adipose-derived stem cells (ASCs) therapy in scleroderma (P < 0.05). SVF gel exhibited the most potent anti-inflammatory and anti-fibrotic effects among treatment groups (P < 0.05). Conclusion SVF gel and CAL significantly reversed dermal sclerosis, and were more effective than CF. The SVF gel group exhibited the lowest inflammation both in transferred fat and in skin lesions, and also the lowest fibrosis among the three groups. This study suggested that SVF gel was a more suitable adipose-based therapy for topical treatment of scleroderma patients.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Mechanical micronization of lipoaspirates for treatment of bleomycin-induced scleroderma in nude mice

Jiang

Wang

Lin

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The application of SVF gel and SVF cells reduced macrophage infiltration in the dermal layer as well as the expression of interleukin-6 mRNA and monocytic chemoattractive protein-1 (MCP-1). In addition, myofibroblast number and collagen formation were reduced in groups using SVF gel and SVF cells [49]. According to these and other researchers, the positive effect in the correction of scars is due to the secretion of various cytokines and growth factors by the progenitor cells of adipose tissue, including hepatocyte growth factor, IL-10 and adrenomedullin which have antifibrotic properties [24,29].…”

Section: Experimental Studies Of Adscs Application For Skin Lesionsmentioning

confidence: 85%

Functional properties and features of clinical application of adipose-derived stem cells in aesthetic medicine and dermatology: a brief review

Ivanishchev¹

2019

COT

View full text Add to dashboard Cite

Clinical and experimental data on the application of adipose stem cells in aesthetic medicine and dermatology were analyzed in this brief review. Adipose tissue is a source of safe and easily accessible autologous stem cells with high regenerative potential. The results of numerous experimental and clinical studies prove the prospect of using adipose derived stem cells for the treatment of various skin diseases, as well as in the complex therapy of chrono-and photoaging of the skin.

show abstract

“…Hence, we postulated that the immunoregulatory capacity of ASCs differed between the 10 4 group and the 10 8 group. Given that the α-SMA level was the same as in the control group, the main function of ASCs in the 10 4 group might be to inhibit excessive secretion of extracellular matrix (ECM) proteins and promote degradation of ECM proteins [48]. In fact, there are several possible mechanisms of ASCs anti brotic effects, including the regulation of TGF-β/Smad axis, the paracrine mechanisms, the antioxidant effects of ASCs and so on [49].…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived stem cells regulate fibrosis by altering CD4+ T-cell immune responses in fat grafting in a mouse model

Chen

Wang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background Autologous fat grafting is becoming increasingly common worldly. However, the long-term retention of fat grafting is still unpredictable due to the inevitable fibrosis that arises during tissue repair. Fibrosis may be regulated by T-cell immune responses that are influenced by adipose-derived stem cells (ASCs). Accordingly, we hypothesized that overly abundant ASCs might promote fibrosis by promoting T-cell immune responses to adipose tissue. Methods We performed 0.3 ml fat grafts with 104/ml, 106/ml and 108/ml ASCs and control group in C57 BL/6 mice in vivo. We observed retention, fibrosis, T-cell immunity, and macrophage infiltration over 12 weeks. In addition, CD4 + T-helper 1 (Th1) cells and T-helper 2 (Th2) cells were co-cultured with ASCs or ASCs conditioned media (CM) in vitro. We detected the ratio of Th2%/Th1% after 24 and 48 hours. Results In vivo, the retention rate was higher in the 104 group, while even lower in the 108 group with significantly increased inflammation and fibrosis than the control group at week 12. There was no significance between control group and the 106 group. Also, the 108 group increased infiltration of M2 macrophages, CD4 + T-cells and Th2/Th1 ratio. In vitro, the ratio of Th2%/Th1% induced by the ASCs-transwell group was higher than the ASCs-CM group and showed concentration-dependent. Conclusions High concentrations of ASCs in adipose tissue can promote Th1–Th2 shifting, and the excess of Th2 cells might promote the persistence of M2 macrophages and increase the level of fibrosis which lead to a decrease in the long-term retention of fat grafts. In addition, we found that ASCs promoted Th1–Th2 shifting in vitro.

show abstract

Mechanical micronization of lipoaspirates for the treatment of hypertrophic scars

Cited by 46 publications

References 29 publications

Mechanical micronization of lipoaspirates for treatment of bleomycin-induced scleroderma in nude mice

Mechanical micronization of lipoaspirates for treatment of bleomycin-induced scleroderma in nude mice

Functional properties and features of clinical application of adipose-derived stem cells in aesthetic medicine and dermatology: a brief review

Adipose-derived stem cells regulate fibrosis by altering CD4+ T-cell immune responses in fat grafting in a mouse model

Contact Info

Product

Resources

About