Mechanical measurements of a single protein molecule and human chromosomes by atomic force microscopy

Ikai, Atsushi; Mitsui, Kiyofumi; Tokuoka, Hirohumi; Xu, Xue Ming

doi:10.1016/s0928-4931(97)00006-4

Cited by 20 publications

(7 citation statements)

References 18 publications

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“…The analysis of individual domain unfolding in terms of the Hook's spring model suggested a spring constant of about 0.01 N/m, which is expected for stretching of flexible backbones governed by an entropic mechanism (Fig. 4) [33]. The persistent length determined from the SFS fitting with the worm-like chain (WLC) model (see examples in Fig.…”

Section: Assignment Of Different Stretching Eventsmentioning

confidence: 94%

Unfolding the multi-length scale domain structure of silk fibroin protein

Shulha

Foo

Kaplan

et al. 2006

Polymer

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Section: Assignment Of Different Stretching Eventsmentioning

confidence: 94%

Unfolding the multi-length scale domain structure of silk fibroin protein

Shulha

Foo

Kaplan

et al. 2006

Polymer

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“…Several biological samples have been characterized from a mechanical point of view by means of AFM. They range from proteins [179] and chromosomes [189] to cells [179,190]. The approach part of the force curve can also provide information on polymer brushing forces [191] and on the electrostatic surface charge of biological samples.…”

Section: Approach Curvementioning

confidence: 99%

AFM: a versatile tool in biophysics

Alessandrini¹,

Facci²

2005

Meas. Sci. Technol.

346

239

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Here we review the applications of atomic force microscopy to the study of samples of biological origin. Emphasis is given to provide the reader with information on the broad range of different biophysical applications that, to date, such a technique can deal with. After recalling briefly the operating principles of an atomic force microscope, the broad field of bio-imaging applications is faced (DNA, DNA-protein interaction, proteins, lipid membranes, cells); thereafter, the use of the atomic force microscope to measure forces is introduced and force mapping on living cells is discussed. This section is followed by the description of the use of force curves in assessing single-molecule inter-and intramolecular interactions. A paragraph on the perspectives of the technique in biophysical applications concludes the paper. We hope that this review can help the reader in appreciating how atomic force microscopy contributes to the current explosive growth of nanobiosciences, where biology, chemistry and physics merge.

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“…15 Its ability to directly image the surface of the IPMC can provide detailed Figure 1 1 H NMR spectra of perfluorocarboxylic acid copolymer.…”

Section: Preparation and Characterizationsmentioning

confidence: 99%

Electrochemical behavior of a new type of perfluorinated carboxylate membrane/platinum composite

Jeong

Kim

2005

J of Applied Polymer Sci

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ABSTRACT:A new type of ionomeric polymer-metal composite (IPMC) based on various compositions of perfluoroalkylacrylate-acryl acid copolymer with different types of counter cation has been synthesized by a radical copolymerization of fluoroalkylacrylate (FA) and acrylic acid (AA). Swelling behavior of the membranes was studied in deionized water as well as in aqueous single salt solutions (LiCl and NaCl). Swell of membrane increased with the increase in ionic content of the copolymer. Swell in deionized water was much higher than in aqueous single salt solution. To examine its performance as a new electroactive polymer, actuation force and displacement responses to DC and step voltage across the IPMC were measured. Results indicated that membranes prepared from 75/25 (FA/AA) (FA25) gave an adequate flexibility and proper hydrated state, allowing easy deflection toward the applied voltage. Surface morphology measurements (atomic force microscopy and scanning electron microscopy) showed that platinum particles are predominantly located at the surfaces.

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Mechanical measurements of a single protein molecule and human chromosomes by atomic force microscopy

Cited by 20 publications

References 18 publications

Unfolding the multi-length scale domain structure of silk fibroin protein

Unfolding the multi-length scale domain structure of silk fibroin protein

AFM: a versatile tool in biophysics

Electrochemical behavior of a new type of perfluorinated carboxylate membrane/platinum composite

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