Mechanical mandible competence in rats with nutritional growth retardation

Lezón, Christian E.; Pintos, Patricia Mabel; Bozzini, Clarisa; Romero, Alan Agüero; Casavalle, Patricia; Friedman, Sílvia; Boyer, P

doi:10.1016/j.archoralbio.2017.03.009

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…The general endurance, stiffness and microarchitecture of the trabecular bone making up the mandible are important to counteract mandibular stresses and strains that occur during biting or chewing [30]. For this reason, the mandible is more useful in metabolic or physiological studies [27, 29, 45].…”

Section: Discussionmentioning

confidence: 99%

Chronic dietary supplementation with kynurenic acid, a neuroactive metabolite of tryptophan, decreased body weight without negative influence on densitometry and mandibular bone biomechanical endurance in young rats

et al. 2019

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Kynurenic acid (KYNA) is a neuroactive metabolite of tryptophan. KYNA naturally occurs in breast milk and its content increases with lactation, indicating the role of neonatal nutrition in general growth with long-term health effects. KYNA is also an antagonist of ionotropic glutamate receptors expressed in bone cells. The aim of this study was to establish the effects of chronic KYNA supplementation on bone homeostasis in young rats, using mandible as a model bone. Female and male newborn Wistar rats were divided into control and KYNA-administered groups until 60 days of age (25x101 mg/L or 25x102 mg/L in drinking water). Hemimandibles were subjected to densitometry, computed tomography analysis and mechanical testing. Rats supplemented with KYNA at both doses showed a decrease in body weight. There were no effects of KYNA administration and mandible histomorphometry. In males, a significant quadratic effect (P < 0.001) was observed in the densitometry of the hemimandible, where BMD increased in the group supplemented with 2.5x101 mg/L of KYNA. Analysis of mechanical tests data showed that when fracture forces were corrected for bone geometry and rats body weight the improvement of bone material properties was observed in male and female rats supplemented with lower dose of KYNA. This study showed that chronic supplementation with KYNA may limit weight gain in the young, without adversely affecting the development of the skeleton.

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Section: Discussionmentioning

confidence: 99%

Chronic dietary supplementation with kynurenic acid, a neuroactive metabolite of tryptophan, decreased body weight without negative influence on densitometry and mandibular bone biomechanical endurance in young rats

et al. 2019

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“…In contrast, thermoxidazed oil consumption has been found to alter cardiovascular status, femur mass and biomechanical competence 6 , 7 . Rat mandible and femur have also been demonstrated to have different performance in growing rats subjected to chronic suboptimal nutrition 12 or in those fed imbalanced diets 24 .…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Mechanical properties of the rat right hemimandible were determined using a three-point bending mechanical test. Before testing, each bone was thawed at room temperature and biomechanical competence was determined as described in our previous publication 12 . Mechanical properties of the rat hemimandible were determined using an Instron model 4442 (Instron Corp., Canton, MA, USA), as described previously 21 .…”

Section: Biomechanical Testing Of the Mandiblementioning

confidence: 99%

“…Mandible quality depends on diet intake and gender 10 . Our previous studies demonstrated impaired mechanical mandible competence in rats fed a suboptimal restricted diet 11,12 and a diet containing inadequate quality or quantity of protein 13 . However, to our knowledge, there are no studies evaluating the effect of different types of dietary fat and the biomechanical competence of the mandible during growth.…”

Section: Introductionmentioning

confidence: 96%

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Effect of fried sunflower oil intake on mandibular biomechanical competence of growing rats

Macri,

Bozzini,

Ferreira-Monteiro

et al. 2023

Acta Odontol Latinoam

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Previous studies by us demonstrated that the consumption of thermally oxidized oil diet adversely affects body growth, lipid metabolism, bone mass and femur biomechanical competence. Aim: The aim of this study was to evaluate the effects of a diet containing fried sunflower oil on the mandible of growing rats. Materials and Method: Male Wistar rats (21±1 day old) (n=21) were assigned at weaning to one of three diets for 8 weeks: a control diet (C), a diet containing sunflower oil (SFO) or a diet containing sunflower oil that had been repeatedly heated (SFOx); both SFO and SFOx were mixed with commercial rat chow at 13% (w/w). The consistency and viscosity of the 3 diets were similar. Zoometrics and food intake were recorded weekly. At wk=8, mandibular growth was assessed by measurements of anatomical points of cleaned bones, and mandible biomechanical competence was assessed to estimate the structural properties of the bone. Statistical analysis was performed by SPSS v. 20.0. Results: Rats fed SFOx diet attained the lowest final body weight (P=0.0074), mandibular weight (P=0.0001) and mandibular \length (P=0.0002). Load bearing capacity (Wf;N), load of yielding (Wy;N) and stiffness (Wy/dy;N/mm) of the mandible were negatively affected by both sunflower oil diets (fresh and fried) (P=0.001; P=0.002; P=0.003, respectively) though SFOx induced the most significant reduction in Wy/dy (C:44.4(5.4) > SFO:36.1(2.1) > SFOx: 26.3(3.7) N/ mm; P=0.003). The deleterious effect of SFOx on mandibular growth was more accentuated on the posterior part of the bone (C:11.4(0.3)=SFO:11.2(0.2)>SFOx: 10.7(0.2) mm; p=0.0005); the anterior/ posterior ratio (C:1.25(0.02)=SFO:1.27(0.02)<SFOx:1.32(0.03); p=0.0001) indicated that SFOx induced mandibular deformation. Conclusion: Consumption of SFOx diet during growth could affect mandibular morphometric properties and biomechanical competence, in terms of bone stiffness.

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