Mechanical force inhibited hPDLSCs proliferation with the downregulation of <i>MIR31HG</i> via DNA methylation

Han, Yineng; Yang, Qingling; Huang, Yiping; Li, Xiaobei; Zhu, Yunyan; Jia, Lingfei; Zheng, Yunfei; Li, Weiran

doi:10.1111/odi.13637

Cited by 15 publications

(16 citation statements)

References 44 publications

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“…Table 3 presents 18 studies that investigated the role of lncRNAs in regulating cellular processes in PDL cells in the presence or absence of stimuli, including studies that compared H-PDL and P-PDL cells [ 60 , 61 , 62 , 63 , 64 , 65 ]. These studies also explored the role of lncRNAs in lipopolysaccharide (LPS)-induced inflammation [ 66 , 67 , 68 , 69 , 70 , 71 ], tumor necrosis factor-alpha (TNF-α)-induced inflammation [ 72 ], mechanical stress [ 73 , 74 , 75 ], hypoxia [ 76 ], and oxidative stress [ 77 ]. To review the outcomes of lncRNA involvement, cell proliferation, apoptosis, inflammatory responses, autophagy, migration, and root resorption were examined.…”

Section: Resultsmentioning

confidence: 99%

“…Inflammation-stimulating factors released by bacteria, such as LPS and TNF-α, activate the immune response in PDL cells, thereby aggravating the destruction of alveolar bone. Among the 18 studies that investigated the regulatory role of lncRNAs in PDL cells in response to inflammation and other stimuli, 6 studies compared the inflammatory responses of H-PDL and P-PDL cells [ 66 , 67 , 68 , 69 , 70 , 71 ], 7 studies stimulated PDL cells with LPS or/and TNF-α to mimic periodontal inflammation [ 66 , 68 , 69 , 70 , 71 , 72 , 74 ], 3 studies stimulated PDL cells with mechanical stress [ 73 , 74 , 75 ], 1 study subjected PDL cells to hypoxia [ 76 ], and 1 study subjected cells to oxidative stress [ 77 ]. Under these stimuli, the biological activities of PDL cells, including cell proliferation, apoptosis, inflammatory responses, osteogenic differentiation, and autophagy, were explored.…”

Section: Discussionmentioning

confidence: 99%

“…PDL cells subjected to mechanical stress are widely used to mimic in vivo conditions. Three lncRNAs, DANCR, MIR31 host gene (MIR31HG), and FER1L4, have been investigated for their role in the regulation of compressive force-induced biological activities in PDL cells [ 73 , 74 , 75 ]. It was suggested that the knockdown of DANCR inhibits the osteoclast formation and root resorption that is induced by compressive force via miR-34a-5p/jagged1 [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

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The Expression and Regulatory Roles of Long Non-Coding RNAs in Periodontal Ligament Cells: A Systematic Review

et al. 2022

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Periodontal ligament (PDL) cells play a pivotal role in periodontal and bone homeostasis and have promising potential for regenerative medicine and tissue engineering. There is compelling evidence that long non-coding RNAs (lncRNAs) are differentially expressed in PDL cells compared to other cell types and that these lncRNAs are involved in a variety of biological processes. This study systematically reviews the current evidence regarding the expression and regulatory functions of lncRNAs in PDL cells during various biological processes. A systematic search was conducted on PubMed, the Web of Science, Embase, and Google Scholar to include articles published up to 1 July 2021. Original research articles that investigated the expression or regulation of lncRNAs in PDL cells were selected and evaluated for a systematic review. Fifty studies were ultimately included, based on our eligibility criteria. Thirteen of these studies broadly explored the expression profiles of lncRNAs in PDL cells using microarray or RNA sequencing. Nineteen studies investigated the mechanisms by which lncRNAs regulate osteogenic differentiation in PDL cells. The remaining 18 studies investigated the mechanism by which lncRNAs regulate the responses of PDL cells to various stimuli, namely, lipopolysaccharide-induced inflammation, tumor necrosis factor alpha-induced inflammation, mechanical stress, oxidative stress, or hypoxia. We systematically reviewed studies on the expression and regulatory roles of lncRNAs in diverse biological processes in PDL cells, including osteogenic differentiation and cellular responses to inflammation, mechanical stress, and other stimuli. These results provide new insights that may guide the development of lncRNA-based therapeutics for periodontal and bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Expression and Regulatory Roles of Long Non-Coding RNAs in Periodontal Ligament Cells: A Systematic Review

et al. 2022

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“…26 Another study employing the same force type and magnitude for PDLCs reported that static compressive force reduced cell proliferation and the Ki67-positive cell population. 27 This phenomenon occurred via regulation of MIR31HG . 27 …”

Section: Discussionmentioning

confidence: 99%

Intermittent compressive force induces cell cycling and reduces apoptosis in embryoid bodies of mouse induced pluripotent stem cells

et al. 2022

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In vitro manipulation of induced pluripotent stem cells (iPSCs) by environmental factors is of great interest for three-dimensional (3D) tissue/organ induction. The effects of mechanical force depend on many factors, including force and cell type. However, information on such effects in iPSCs is lacking. The aim of this study was to identify a molecular mechanism in iPSCs responding to intermittent compressive force (ICF) by analyzing the global gene expression profile. Embryoid bodies of mouse iPSCs, attached on a tissue culture plate in 3D form, were subjected to ICF in serum-free culture medium for 24 h. Gene ontology analyses for RNA sequencing data demonstrated that genes differentially regulated by ICF were mainly associated with metabolic processes, membrane and protein binding. Topology-based analysis demonstrated that ICF induced genes in cell cycle categories and downregulated genes associated with metabolic processes. The Kyoto Encyclopedia of Genes and Genomes database revealed differentially regulated genes related to the p53 signaling pathway and cell cycle. qPCR analysis demonstrated significant upregulation of Ccnd1, Cdk6 and Ccng1. Flow cytometry showed that ICF induced cell cycle and proliferation, while reducing the number of apoptotic cells. ICF also upregulated transforming growth factor β1 (Tgfb1) at both mRNA and protein levels, and pretreatment with a TGF-β inhibitor (SB431542) prior to ICF abolished ICF-induced Ccnd1 and Cdk6 expression. Taken together, these findings show that TGF-β signaling in iPSCs enhances proliferation and decreases apoptosis in response to ICF, that could give rise to an efficient protocol to manipulate iPSCs for organoid fabrication.

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“…The cell fractionation assays were performed using a Nuclei Isolation Kit (Invent Biotechnologies, Beijing, China) to separate cytoplasmic and nuclear RNA as previously described [ 20 ]. Briefly, cytoplasm extraction buffer was added to the harvested cells with vigorous vortexing for 15 s. After incubation on ice for 5 min, the mixtures were centrifuged at top speed in a microcentrifuge at 4 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Long non-coding RNA SNHG5 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the miR-212-3p/GDF5/SMAD pathway

et al. 2022

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Background The treatment of bone loss has posed a challenge to clinicians for decades. Thus, it is of great significance to identify more effective methods for bone regeneration. However, the role and mechanisms of long non-coding RNA small nucleolar RNA host gene 5 (SNHG5) during osteogenic differentiation remain unclear. Methods We investigated the function of SNHG5, Yin Yang 1 (YY1), miR-212-3p and growth differentiation factor 5 (GDF5) in osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) in vitro and in vivo. Molecular mechanisms were clarified by chromatin immunoprecipitation assay and dual luciferase reporter assay. Results We found SNHG5 expression was upregulated during osteogenesis of hBMSCs. Knockdown of SNHG5 in hBMSCs inhibited osteogenic differentiation while overexpression of SNHG5 promoted osteogenesis. Moreover, YY1 transcription factor directly bound to the promoter region of SNHG5 and regulated SNHG5 expression to promote osteogenesis. Dual luciferase reporter assay confirmed that SNHG5 acted as a miR-212-3p sponge and miR-212-3p directly targeted GDF5 and further activated Smad1/5/8 phosphorylation. miR-212-3p inhibited osteogenic differentiation, while GDF5 promoted osteogenic differentiation of hBMSCs. In addition, calvarial defect experiments showed knockdown of SNHG5 and GDF5 inhibited new bone formation in vivo. Conclusion Our results demonstrated that the novel pathway YY1/SNHG5/miR-212-3p/GDF5/Smad regulates osteogenic differentiation of hBMSCs and may serve as a potential target for the treatment of bone loss.

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Mechanical force inhibited hPDLSCs proliferation with the downregulation of MIR31HG via DNA methylation

Cited by 15 publications

References 44 publications

The Expression and Regulatory Roles of Long Non-Coding RNAs in Periodontal Ligament Cells: A Systematic Review

The Expression and Regulatory Roles of Long Non-Coding RNAs in Periodontal Ligament Cells: A Systematic Review

Intermittent compressive force induces cell cycling and reduces apoptosis in embryoid bodies of mouse induced pluripotent stem cells

Long non-coding RNA SNHG5 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the miR-212-3p/GDF5/SMAD pathway

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