Mechanical dynamics of single cells during early apoptosis

Pelling, Andrew E.; Veraitch, Farlan; Chu, Carlton; Mason, Chris; Horton, Michael A.

doi:10.1002/cm.20391

Cited by 85 publications

(112 citation statements)

References 58 publications

Supporting

Mentioning

102

Contrasting

Order By: Relevance

“…Cuerrier et al [18] investigated the mechanical properties of human umbilical vein endothelial cells before and after drug treatment. Pelling et al [19] investigated the mechanical properties of human neonatal foreskin fibroblasts during early apoptosis by combining confocal microscopy and AFM. These studies were performed with conventional nanoscale AFM probe tips that are relatively sharp, and thus may cause damage to delicate cells.…”

mentioning

confidence: 99%

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

Liu

et al. 2012

Sci. China Life Sci.

View full text Add to dashboard Cite

Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt's lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a micro-sphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young's modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young's modulus than that of indolent cancer cells, which may improve our understanding of metastasis. atomic force microscopy, red blood cell, cancer cell, mechanical properties, Young's modulus Citation:Li M, Liu L Q, Xi N, et al. Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells. Sci China Life Sci, 2012, 55: 968 -973,

show abstract

mentioning

confidence: 99%

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

Liu

et al. 2012

Sci. China Life Sci.

View full text Add to dashboard Cite

show abstract

“…Vinculin was labeled using 0.5% monoclonal anti-vinculin primary antibody produced in mouse (Sigma) and 0.5% rabbit anti-mouse IgG secondary antibody conjugated to AlexaFluor 546 fluorophore. DNA was labeled with 0.5% DAPI (Invitrogen) [21].…”

Section: Immunofluorescencementioning

confidence: 99%

“…The AFM was invented in 1986 as a scanning probe microscope (SPM) for the purposes of imaging [16] and has since been applied to biology for both imaging and measuring the mechanical properties of living cells [17][18][19][20][21]. Further insight into the mechanics of the CSK may be studied by the integration of AFM with optical imaging systems such as the ISRN Cell Biology 3 fluorescence microscope [19,22].…”

Section: Introductionmentioning

confidence: 99%

“…This is crucial in allowing the observation of real-time responses of the CSK to mechanical stimuli, as opposed to fixing and staining the sample after force measurements using the AFM [17]. The technology of AFM/optical microscopy integration has opened the door to observing the immediate response of the CSK to an external force applied by the AFM, such that the transmission of force through the CSK, which often occurs within the seconds to minutes after a mechanical stimulus [21]. This method of visualizing the CSK using laser scanning confocal microscopy (LSCM) while applying external force with the AFM will be used in our study to observe the immediate response of the vimentin CSK to indentation by the AFM.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces

Wang

Pelling

2012

ISRN Cell Biology

Self Cite

View full text Add to dashboard Cite

The intermediate filament (IF) cytoskeleton plays an important role in integrating biomechanical pathways associated with the actin and microtubule cytoskeleton. Vimentin is a type III IF protein commonly found in fibroblast cells and plays a role in transmitting forces through the cytoskeleton. Employing simultaneous laser scanning confocal and atomic force microscopy (AFM), we developed a methodology to quantify the deformation of the GFP-vimentin-labeled IF cytoskeleton as a function of time in response to force application by the AFM. Over short times (seconds), IFs deformed rapidly and transmitted force throughout the entire cell in a highly complex and anisotropic fashion. After several minutes, mechanically induced displacements of IFs resemble basal movements. In well-adhered cells the deformation of IFs is highly anisotropic as they tend to deform away from the longitudinal axis of the cell. This study demonstrates that simultaneous AFM and LSCM can be employed to track the deformation and dissipation of force through the IF cytoskeleton.

show abstract

“…In fact, fundamental biological processes such as apoptosis (cell death), mitosis, cancer metastasis, and stem cell fate are known to be modulated and even controlled by such mechanical cues [1,5,8,[10][11][12]18]. Wang and Pelling [22] showed that the nanomechanical breakdown of cells within a monolayer is governed by the depolymerization of F-actin within the cells, while the extracellular matrix (ECM) maintains the integrity of the sheet.…”

mentioning

confidence: 99%

Micro and nanotechnology for biological and biomedical applications

Lim

Han

Guck

et al. 2010

Med Biol Eng Comput

View full text Add to dashboard Cite

This special issue contains some of the current state-of-the-art development and use of micro and nanotechnological tools, devices and techniques for both biological and biomedical research and applications. These include nanoparticles for bioimaging and biosensing, optical and biophotonic techniques for probing diseases at the nanoscale, micro and nano-fabricated tools for elucidating molecular mechanisms of mechanotransduction in cell and molecular biology and cell separation microdevices and techniques for isolating and enriching targeted cells for disease detection and diagnosis. Although some of these works are still at the research stage, there is no doubt that some of the important outcomes will eventually see actual biomedical applications in the not too distant future.The last decade has seen increasing use of micro and nanotechnology for biological and biomedical applications. This can be attributed to several reasons. Firstly, there is tremendous advancement in the research and development of micro and nanotechnological tools and devices. Secondly, much progress in cell and molecular biology and biomedical sciences has also been made. Thirdly, there is a huge push towards multidisciplinary research that involves close collaboration among researchers from several disciplines including physics, chemistry, engineering, biology, and medicine. In fact, it is through this collaboration that we are starting to see how micro and nanotechnology are catalyzing and even accelerating research in the biological and biomedical sciences. Over the last 100 years, technology has always been seen to play an important contributing role in advancing biological and biomedical research and to its eventual applications in the clinics and hospitals. Thus, it is not surprising for micro and nanotechnology to play the same role and make similar contributions as well.The development of micro and nanotechnological tools and devices can now enable us to image and probe cells and biomolecules that were previously impossible. We can now easily characterize the minute responses of individual cells and biomolecules in the micro, nano, and even picoscale range when subjected to mechanical, electrical, magnetic, and/or biochemical stimuli. In fact, micro and nanotechnology do offer several advantages over current conventional macroscale technology for any such test, experiment or application. These advantages include: (i) only a minute amount of sample and testing agents are needed; (ii) fast processing time as analysis time is reduced due to minute amount of sample being tested; (iii) highly sensitive and increased accuracy; (iv) increased portability, especially for biomedical application where there is

show abstract

Mechanical dynamics of single cells during early apoptosis

Cited by 85 publications

References 58 publications

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces

Micro and nanotechnology for biological and biomedical applications

Contact Info

Product

Resources

About