Mechanical Criterion for the Rupture of a Cell Membrane under Compression

Gonzalez‐Rodriguez, David; Guillou, Lionel; Cornat, François; Lafaurie-Janvore, Julie; Babataheri, Avin; Langre, Emmanuel de; Barakat, Abdul I.; Husson, Julien

doi:10.1016/j.bpj.2016.11.001

Cited by 37 publications

(22 citation statements)

References 51 publications

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“…Assuming the lysosomal radius~500 nm (Figure 2a) and membrane thickness of~10 nm [43], we roughly estimated the hoop stress to be about 15 kPa. This estimation is perfectly in line with the recently measured 12 kPa critical pressure required for membrane rupture in endothelial cells [44].…”

Section: Pmf Stimulation Of Spion-loaded Cells Results In Cell Deathsupporting

confidence: 91%

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Lunov

Uzhytchak

Smolková

et al. 2019

Cancers

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Lysosome-activated apoptosis represents an alternative method of overcoming tumor resistance compared to traditional forms of treatment. Pulsed magnetic fields open a new avenue for controlled and targeted initiation of lysosomal permeabilization in cancer cells via mechanical actuation of magnetic nanomaterials. In this study we used a noninvasive tool; namely, a benchtop pulsed magnetic system, which enabled remote activation of apoptosis in liver cancer cells. The magnetic system we designed represents a platform that can be used in a wide range of biomedical applications. We show that liver cancer cells can be loaded with superparamagnetic iron oxide nanoparticles (SPIONs). SPIONs retained in lysosomal compartments can be effectively actuated with a high intensity (up to 8 T), short pulse width (~15 µs), pulsed magnetic field (PMF), resulting in lysosomal membrane permeabilization (LMP) in cancer cells. We revealed that SPION-loaded lysosomes undergo LMP by assessing an increase in the cytosolic activity of the lysosomal cathepsin B. The extent of cell death induced by LMP correlated with the accumulation of reactive oxygen species in cells. LMP was achieved for estimated forces of 700 pN and higher. Furthermore, we validated our approach on a three-dimensional cellular culture model to be able to mimic in vivo conditions. Overall, our results show that PMF treatment of SPION-loaded lysosomes can be utilized as a noninvasive tool to remotely induce apoptosis.

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Section: Pmf Stimulation Of Spion-loaded Cells Results In Cell Deathsupporting

confidence: 91%

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Lunov

Uzhytchak

Smolková

et al. 2019

Cancers

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“…The brainstems were then placed in a vacuum chamber for 10 min to reduce free air in the lumina of the blood vessels. Ultimate maximum pressure within the vacuum chamber was set to 1350 mmHg to prevent cell membrane damages (Gonzalez-Rodriguez et al 2016 ; Holzapfel et al 2005 ), although the maximum pressure did not exceed 500 mmHg during the experiments as this was not necessary to remove air bubbles. Finally, each brainstem was placed in a 100 ml syringe, filled with Fomblin, for MR scanning.…”

Section: Methodsmentioning

confidence: 99%

Ex vivo visualization of the trigeminal pathways in the human brainstem using 11.7T diffusion MRI combined with microscopy polarized light imaging

et al. 2018

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Classic anatomical atlases depict a contralateral hemispheral representation of each side of the face. Recently, however, a bilateral projection of each hemiface was hypothesized, based on animal studies that showed the coexistence of an additional trigeminothalamic tract sprouting from the trigeminal principal sensory nucleus that ascends ipsilaterally. This study aims to provide an anatomical substrate for the hypothesized bilateral projection. Three post-mortem human brainstems were scanned for anatomical and diffusion magnetic resonance imaging at 11.7T. The trigeminal tracts were delineated in each brainstem using track density imaging (TDI) and tractography. To evaluate the reconstructed tracts, the same brainstems were sectioned for polarized light imaging (PLI). Anatomical 11.7T MRI shows a dispersion of the trigeminal tract ( tt ) into a ventral and dorsal portion. This bifurcation was also seen on the TDI maps, tractography results and PLI images of all three specimens. Referring to a similar anatomic feature in primate brains, the dorsal and ventral tracts were named the dorsal and ventral trigeminothalamic tract ( dtt and vtt ), respectively. This study shows that both the dtt and vtt are present in humans, indicating that each hemiface has a bilateral projection, although the functional relevance of these tracts cannot be determined by the present anatomical study. If both tracts convey noxious stimuli, this could open up new insights into and treatments for orofacial pain in patients.

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“…On the other hand, there were significant differences in enucleation efficiency. A cytoplasmic membrane is composed of phospholipid bilayers and can be recovered by fluid movement even with slight damage [ 27 ], however, it can be ruptured under high compressive stress [ 28 ]. Pulled Pasteur pipettes were made by hand by the researcher, therefore, they are wider in diameter than micromanipulator enucleation needles, which can further damage the cytoplasm by inconsistent pressure.…”

Section: Discussionmentioning

confidence: 99%

Application of the modified handmade cloning technique to pigs

Lee¹,

Ji²,

Lee³

et al. 2021

J Anim Sci Technol

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Although somatic cell nuclear transfer (SCNT) is frequently employed to produce cloned animals in laboratories, this technique is expensive and inefficient. Therefore, the handmade cloning (HMC) technique has been suggested to simplify and advance the cloning process, however, HMC wastes many oocytes and leads to mitochondrial heteroplasmy. To solve these problems, we propose a modified handmade cloning (mHMC) technique that uses simple laboratory equipment, i.e., a Pasteur pipette and an alcohol lamp, applying it to porcine embryo cloning. To validate the application of mHMC to pig cloning, embryos produced through SCNT and mHMC are compared using multiple methods, such as enucleation efficiency, oxidative stress, embryo developmental competence, and gene expression. The results show no significant differences between techniques except in the enucleation efficiency. The 8-cell and 16-cell embryo developmental competence and Oct4 expression levels exhibit significant differences. However, the blastocyst rate is not significantly different between mHMC and SCNT. This study verifies that cloned embryos derived from the two techniques exhibit similar generation and developmental competence. Thus, we suggest that mHMC could replace SCNT for simpler and cheaper porcine cloning.

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Mechanical Criterion for the Rupture of a Cell Membrane under Compression

Cited by 37 publications

References 51 publications

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

Ex vivo visualization of the trigeminal pathways in the human brainstem using 11.7T diffusion MRI combined with microscopy polarized light imaging

Application of the modified handmade cloning technique to pigs

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