Mechanical competence of ovariectomy-induced compromised bone after single or combined treatment with high-frequency loading and bisphosphonates

Abstract

Osteoporosis leads to increased bone fragility, thus effective approaches enhancing bone strength are needed. Hence, this study investigated the effect of single or combined application of high-frequency (HF) loading through whole body vibration (WBV) and alendronate (ALN) on the mechanical competence of ovariectomy-induced osteoporotic bone. Thirty-four female Wistar rats were ovariectomized (OVX) or sham-operated (shOVX) and divided into five groups: shOVX, OVX-shWBV, OVX-WBV, ALN-shWBV and ALN-WBV. (Sham)WBV loading was applied for 10 min/day (130 to 150 Hz at 0.3g) for 14 days and ALN at 2 mg/kg/dose was administered 3x/week. Finite element analysis based on micro-CT was employed to assess bone biomechanical properties, relative to bone micro-structural parameters. HF loading application to OVX resulted in an enlarged cortex, but it was not able to improve the biomechanical properties. ALN prevented trabecular bone deterioration and increased bone stiffness and bone strength of OVX bone. Finally, the combination of ALN with HF resulted in an increased cortical thickness in OVX rats when compared to single treatments. Compared to HF loading, ALN treatment is preferred for improving the compromised mechanical competence of OVX bone. In addition, the association of ALN with HF loading results in an additive effect on the cortical thickness.

Is the Jaw Bone Micro-Structure Altered in Response to Osteoporosis and Bisphosphonate Treatment? A Micro-CT Analysis

“…SMI is used to measure the rods and plates in the trabecular bone and gives information about the change in surface curvature that happens when a structure switches between an ideal plate-like to cylindrical or planar shape. In line with the results observed in this study, previous studies also indicate that in OVX rats, there is a trabeculae shift from a plate-like to a rod-like shape resulting in loss of trabecular connectivity and micro-architecture deterioration [ 66 , 67 ]. Furthermore, the treatment with ALN resulted in bone anabolic findings, mainly for Tb.Sp, Tb.N Tb.Pf, and SMI in the condyle.…”

Protective Effects of Virgin Coconut Oil and Tocotrienol-Rich Fraction on Bone Biomechanics

“…The OVX group showed deterioration in bone ductility compared to the sham-operated group. This result matched with previous study which reported that decreased oestrogen level via ovariectomy led to decreased in cortical bone strength [44]. There was an increasing trend of displacement value between the treated groups (OVX+V, OVX+T and OVX+VT) compared to the OVX group.…”

Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone

“…RANKL secreted by osteoblasts promotes osteoclast differentiation 38 , and failure in osteoclastogenesis ameliorates bone formation 39 , meaning that the disruption of the activities of one of the two cell lineages always leads to the dysfunction of the other cell lineage. For example, the antiresorptive drug bisphosphonate also plays an inhibitory role in osteogenesis 40 , and anabolic agent PTH simultaneously promotes bone resorption 8 , both of which potentially risk aggravating osteoporosis, even leading to bone fracture; as a solution, combinative treatment is more effective in the enhancement of bone quality 41 . Therefore, the simultaneous antiresorptive and anabolic role of naringin shows great value in clinical disused osteoporosis treatment.…”