Mechanical and physio-biological properties of peptide-coated stent for re-endothelialization

Abstract

Background: The aim of this study was to characterize the mechanical and physio-biological properties of peptidecoated stent (PCS) compared to commercialized drug-eluting stents (DESs). Methods: WKYMVm (Trp-Lys-Tyr-Met-Val-D-Met), a stimulating peptide for homing endothelial colony-forming cell was specially synthesized and coated to bare metal stent (BMS) by dopamine-derived coordinated bond. Biological effects of PCS were investigated by endothelial cell proliferation assay and pre-clinical animal study. And mechanical properties were examined by various experiment. Results: The peptide was well-coated to BMS and was maintained and delivered to 21 and 7 days in vitro and in vivo, respectively. Moreover, the proliferation of endothelial cell in PCS group was increased (approximately 36.4 ± 5.77%) in PCS group at 7 day of culture compare to BMS. Although, the radial force of PCS was moderated among study group. The flexibility of PCS was (0.49 ± 0.082 N) was greatest among study group. PCS did not show the outstanding performance in recoil and foreshortening test (3.1 ± 0.22% and 2.1 ± 0.06%, respectively), which was the reasonable result under the guide line of FDA (less than 7.0%). The nominal pressure (3.0 mm in a diameter) of PCS established by compliance analysis was 9 atm. The changing of PCS diameter by expansion was similar to other DESs, which is less than 10 atm of pressure for the nominal pressure. Conclusions: These results suggest that the PCS is not inferior to commercialized DES. In addition, since the PCS was fabricated as polymer-free process, secondary coating with polymer-based immunosuppressive drugs such as-limus derivatives may possible.

Reduced restenosis and enhanced re-endothelialization of functional biodegradable vascular scaffolds by everolimus and magnesium hydroxide

“…Author details 1 Department of Biomedical Science, CHA University, 335 Pangyo-Ro, Bundang-Gu, Seongnam-Si, Gyeonggi 13488, Korea. 2 Department of Biomedical Engineering, SKKU Institute for Convergence, Sungkyunkwan University (SKKU), 2066 Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi 16419, Korea. 3 Department of Intelligent Precision Healthcare Convergence, SKKU Institute for Convergence, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi 16419, Korea.…”

“…To treat these lethal diseases, the vascular stent has been commonly used to restore blood flow. First-generation bare metal stents (BMSs), consisting of cobalt-chromium (Co-Cr) and stainless steel support the lining of the blood vessels with high mechanical properties but have been frequently reported to induce in stent restenosis (ISR) and thrombosis due to foreignbody reactions [1][2][3]. Second-generation drug-eluting stents (DESs) have been developed from BMSs to overcome this problem and provide additional drug release.…”

A Study of PLA Thin Film on SS 316L Coronary Stents Using a Dip Coating Technique

“…Additionally, it has shown excellent mechanical properties compared to other synthetic polymers at a more affordable price [ 20 , 21 ]. It is essential to select an adequate material for use on stents because there are many requirements that must be met, including that the material should be non-cytotoxic, resorbable, flexible, radiopaque, biocompatible, non-erodible, and it should display excellent hemocompatibility, controlled drug encapsulation and release, and sufficient radial strength [ 5 , 8 , 21 , 22 ].…”

Biofunctionalization of cardiovascular stents to induce endothelialization: Implications for in- stent thrombosis in diabetes

“…Peptides targeting endothelial cells have also been investigated, including REDV (Arginine-Glutamate-Aspartate-Valine) ( Hubbell et al, 1991 ). Specific peptides to EPCs have been identified such as the disulfide cyclic octa-peptide (cGRGDdvc, also known as LXW7) ( Hao et al, 2017 ), TPS (Threonine-Proline-Serine-Leucine-Glutamate-Glutamine-Arginine-Threonine-Valine-Tyrosine-Alanine-Lysine) ( Veleva et al, 2007 ), and WKYMVm (Trp-Lys-Tyr-Met-Val-D-Met) ( Bae et al, 2020a ). These peptides interact with the integrins -which are adhesion receptors on the cells - and activate them, resulting in enhanced cell adhesion and binding.…”