“…PLA attachments by FFF have a high surface roughness, which can favor the adhesion of microorganisms since rougher surfaces tend to promote higher biofilm accumulation ( Alp et al, n.d. ; Batak et al, 2021; de Campos et al, 2023 , de Campos and dos Reis, 2023 , Mei et al, 2011 , Vidakis et al, 2020 ), due to the increase of the surface area or being associated with hydrophobicity, electrostatic interactions, and Van Der Waals forces that benefit microbial adhesion (Batak et al, 2021; Vidakis et al, 2020 ). Surface roughness has a major influence on microbial adhesion after the period of biofilm establishment because the microbes can find the best spaces to adhere, such as cracks and pores ( Mei et al, 2011 ).…”