INTRODUCTIONAlthough many intrinsic and extrinsic factors are involved in the etiology and pathogenesis of dental caries, pH in the oral cavity is known to be one of the main factors. In particular, fluctuations in dental plaque pH after carbohydrate consumption exacerbate caries occurrence and severity. As shown by Stephan 1) , the pH value following sucrose intake drops rapidly to its minimum level (which is below pH 5.0) , and then over a period of 30 minutes to several hours, the pH value slowly returns to its original value (which is above pH 7.0) . As a result, pH in the oral cavity is subjected to successive fluctuations between pH below 5 and then above 7 with each carbohydrate intake. Such a pH fluctuation occurs because metabolism of fermentable substrates by the plaque flora leads to acid production
2); following which, acid dilution and/or neutralization occurs according to the flow rate of saliva and its buffering capacity 3) . It should also be highlighted that these pH fluctuations play a key role in de-and remineralization of teeth. This is because enamel dissolution rate is reduced when pH value is increased
4), and when pH is sustained above 5.5 both hydroxyapatite(HAP)and fluorapatite(FAP)are synthesized. Moreover, when pH is between 4.5 and 5.5, HAP is dissolved, but FAP synthesis is still in progress if fluoride exists 5) . Caries formation is a dynamic process that involves alternating periods of de-and remineralization of the teeth with pH changes. When the rate of remineralization exceeds the rate of ion transport out of the tooth surface, the surface layer can be retained with the continuous renewal. However, if demineralization is the predominant process over a defined period, or that the rate of demineralization exceeds the rate of remineralization, the net result will be a gradational loss of tooth minerals leading to irreversible cavity formation. Thus, the outbreak occurrence and progression of a caries lesion are associated with an imbalance between de-and remineralization. In various researches 6-9) , protocols have been designed to investigate caries progression using a pH cycle in which the teeth were immersed alternately and periodically into two different pH solutions. Those studies indicated that the rate of demineralization process was highly dependent on the low pH and ion concentration of such ions as Ca 2+ , PO 4 3-, and F -of demineralization solutions 10,11) . Among the ions mentioned above, fluoride is known to have anticariogenic effect. As such, there are many studies concerning fluoride release from fluoride-containing materials 12-15) . However, most of the studies performed did not indicate the influence of demineralizationremineralization imbalance on the teeth in human oral conditions, as shown in Stephan's curve under changing pH. Therefore, in the clinical setting, a direct correlation between pH changes simulating oral cavity conditions and caries formation is still a matter of discussion.For the purpose of observing caries occurrence The aim of this...