Mechanical and biological properties of oxidized horn keratin

Zhang, Quanbin; Guanghua, Shan; Cao, Ping; He, Jun; Lin, Zhongshi; Huang, Yao‐Xiong; Ao, Ningjian

doi:10.1016/j.msec.2014.11.051

Cited by 30 publications

(25 citation statements)

References 59 publications

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“…In the zone of denaturation of KIF, the peak is shifted slightly to higher temperature compared to the SB thermogram; however in the zone of melting of KAPs the temperature shifts to lower values in comparison to untreated keratin (SB). Similar results have been found for other types of keratin after oxidation with hydrogen peroxide [52]. Thus, it is evident that chemical treatment with NaOH (in this research) or oxidation by other chemical treatments [52] produce comparable degradation of the surface structure; in this case some inter-macrofibrillar matrix segments are diminished or deteriorated.…”

Section: Differential Scanning Calorimetrysupporting

confidence: 84%

“…Similar results have been found for other types of keratin after oxidation with hydrogen peroxide [52]. Thus, it is evident that chemical treatment with NaOH (in this research) or oxidation by other chemical treatments [52] produce comparable degradation of the surface structure; in this case some inter-macrofibrillar matrix segments are diminished or deteriorated. However, the melting temperature of the intermediate filaments is not affected by treatment.…”

Section: Differential Scanning Calorimetrysupporting

confidence: 84%

See 1 more Smart Citation

Chitosan–Starch–Keratin Composites: Improving Thermo-Mechanical and Degradation Properties Through Chemical Modification

et al. 2017

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modified ground quill, 2522% for composites with modified long fiber and 3206% for the composites with modified short fiber. The lysozyme test shows an improved in the degradability rate, the weight loss of the films at 21 days is reduced from 73% for chitosan-starch matrix up to 16% for the composites with 5 wt% of quill; but all films show a biodegradable character depending on keratin type and chemical modification. The outstanding properties related to the addition of treated keratin materials show that these natural composites are a remarkable alternative to potentiating chitosan-starch films with sustainable features.

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Section: Differential Scanning Calorimetrysupporting

confidence: 84%

Section: Differential Scanning Calorimetrysupporting

confidence: 84%

Chitosan–Starch–Keratin Composites: Improving Thermo-Mechanical and Degradation Properties Through Chemical Modification

et al. 2017

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“…Several keratins have been used as prognostic predictors for different carcinomas of epithelial origin 24 – 26 . Studies have demonstrated that keratins play a vital role in maintaining the mechanical stability and integrity of epithelial cells, and participate in several intracellular signaling pathways involved in cell stress, proliferation, and metastasis 27 , 28 . Numerous studies have found several genes that were specifically upregulated or downregulated in CRC tissues and could be used as biomarkers for diagnosis or prognosis 25 , 29 .…”

Section: Discussionmentioning

confidence: 99%

Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway

Liu

et al. 2018

Cell Death Dis

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Little is known about the function of Keratin 80 (KRT80), an epithelial keratin, in cancer. This study investigated the role of KRT80 in the prognosis of colorectal carcinoma (CRC) and the underlying mechanisms involved in CRC migration and invasion. We analyzed the expression of KRT80 using The Cancer Genome Atlas and Oncomine databases. Higher expression of KRT80 was found to be significantly associated with multiple pathological parameters, lower disease-free survival, and overall survival in CRC patients. Also, KRT80 was an independent prognostic indicator for CRC. Furthermore, altered KRT80 expression impacted migration and invasion of CRC cells, as well as the expression of epithelial–mesenchymal transition (EMT)-related markers and cell morphology via the AKT pathway. Inhibiting the expression of AKT could reverse these phenomena. Liquid Chromatograph Mass Spectrometer/Mass Spectromete, Co-immunoprecipitation, and laser scanning confocal microscopy techniques showed that KRT80 could interact with protein kinase, DNA-activated, catalytic polypeptide (PRKDC). Suppressing PRKDC could inhibit the expression of AKT and EMT, as well as the migration and invasion of CRC cells. Taken together, these results demonstrated that KRT80 was an independent prognostic biomarker for CRC and promoted CRC migration and invasion by interacting with PRKDC via activation of the AKT pathway.

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“…In our previous research, [5,6] we studied the microstructure and mechanical properties of horns from three domestic bovids (buffalo, cattle and sheep), and the chemical composition of keratin of the horns to fill in gaps in the understanding of the material. We found that the mechanical properties of the three horns are slightly different owing to their differences in the secondary structure of keratins especially in the disulfide region.…”

Section: Introductionmentioning

confidence: 99%

Raman and surface‐enhanced Raman spectroscopy on horns and their correlation with biomechanical properties

Zhou¹,

Pan²,

Wang³

et al. 2016

J Raman Spectroscopy

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Recently horns are considered as potential candidates for various biomedical applications because of their distinct biomechanical properties. We therefore performed Raman spectral analysis and mechanical test on the horns of buffalo, cattle and sheep to obtain information about the molecular compositions in various parts of the horns and their correlation with biomechanical properties. We also developed a surface enhanced Raman spectroscopy (SERS) to trace their degradation products. We found that various parts of the horns have similar overall molecular compositions, but have differences in the bands of C-C-S-S-C-C conformation, α-helix and β-sheet conformation, especially in the bands of 507-515 cm À1 which reflect the relative sulfur content and the structural conformation of the disulfide linkages. The Raman intensities at the region are highly correlated with the Young's modulus and tensile strength of the horns, indicating that the biomechanical properties of the horns are mainly determined by their disulfide bonds. It also suggests that Raman spectroscopy has the potential to perform non-destructive detection on the mechanical strength of horns. The SERS measurements verified that the main constituents of the degradation products from both the buffalo and sheep horns were amino acids and polypeptides, suggesting that the horns are suitable candidates for biomaterial scaffolds.

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Mechanical and biological properties of oxidized horn keratin

Cited by 30 publications

References 59 publications

Chitosan–Starch–Keratin Composites: Improving Thermo-Mechanical and Degradation Properties Through Chemical Modification

Chitosan–Starch–Keratin Composites: Improving Thermo-Mechanical and Degradation Properties Through Chemical Modification

Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway

Raman and surface‐enhanced Raman spectroscopy on horns and their correlation with biomechanical properties

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