Mechanical and antibacterial properties of polymethyl methacrylate modified with zinc dimethacrylate

An, Jiali; Ding, Ning; Zhang, Zutai

doi:10.1016/j.prosdent.2022.04.029

Cited by 16 publications

(11 citation statements)

References 32 publications

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“…The CCK-8 cell motility assay kit contains the reagent WST-8, which is reduced to a highly water-soluble yellow formazan dye by the cell dehydrogenase under the action of the electron carrier 1-methoxy-5-methylphenazine-dimethyl ester sulfate (1-Methoxy PMS). Its color depth is directly proportional to cell metabolic activity ( An et al, 2022 ). As shown in Figure 6 , Compared with the control group, 0.5, 1.0, 2.0, and 4.0 mg/mL of KSE reduced the metabolic activity of V. parahaemolyticus during the biofilm formation by 67.97, 77.98, 83.31, and 88.70%, respectively, which was higher than that of mature biofilm, that is 63.71, 74.46, 80.21, and 83.39%, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Ultrasonic-assisted extraction, anti-biofilm activity, and mechanism of action of Ku Shen (Sophorae Flavescentis Radix) extracts against Vibrio parahaemolyticus

Zhao,

Guo,

et al. 2024

Front. Microbiol.

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The objective of this study is to optimize the ultrasonic-assisted extraction process of Ku Shen (Sophorae Flavescentis Radix) extracts (KSE) against Vibrio parahaemolyticus and explore their anti-biofilm activity and mechanism of action. The ultrasonic-assisted extraction process of KSE optimized by single factor experiment, Box–Behnken design and response surface methodology was as follows: 93% ethanol as solvent, liquid/material ratio of 30 mL/g, ultrasonic power of 500 W, extraction temperature of 80°C and time of 30 min. Under these conditions, the diameter of inhibition circle of KSE was 15.60 ± 0.17 mm, which had no significant difference with the predicted value. The yield of dried KSE is 32.32 ± 0.57% and the content of total flavonoids in KSE was 57.02 ± 5.54%. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of KSE against V. parahaemolyticus were 0.25 and 0.5 mg/mL, respectively. Crystal violet staining, Congo red plate, spectrophotometry, CCK-8 and scanning electron microscopy were used to investigate the activity and mechanism of action of KSE against V. parahaemolyticus biofilm. The results showed that the sub-MIC of KSE could significantly inhibit biofilm formation, reduce the synthesis of polysaccharide intercellular adhesin (PIA) and the secretion of extracellular DNA. In addition, the inhibition rate of biofilm formation and clearance rate of mature biofilm of 1.0 mg/mL KSE were 85.32 and 74.04%, and the reduction rate of metabolic activity of developing and mature biofilm were 77.98 and 74.46%, respectively. These results were confirmed by visual images obtained by scanning electron microscopy. Therefore, KSE has the potential to further isolate the anti-biofilm agent and evaluate it for the preservation process of aquatic products.

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Section: Resultsmentioning

confidence: 99%

“…The effect of KSE on biofilm metabolic activity of V. parahaemolyticus was determined by CCK-8 assay ( An et al, 2022 ). For the inhibition of the metabolic activity during biofilm formation, overnight cultures of V. parahaemolyticus were adjusted to approximately 10 6 cells/mL and inoculated into 96-well microtiter plates (180 μL/well).…”

Section: Methodsmentioning

confidence: 99%

Ultrasonic-assisted extraction, anti-biofilm activity, and mechanism of action of Ku Shen (Sophorae Flavescentis Radix) extracts against Vibrio parahaemolyticus

Zhao,

Guo,

et al. 2024

Front. Microbiol.

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“…7E, the BNN/TiO 2 nanocomposite system–modified flow resin simultaneously possesses a higher FS improvement rate and excellent antibacterial properties, showing superior capacity. 7,9,23,24,31,49–55 The absence of cytotoxicity is a necessary condition for the BNN/TiO 2 nanocomposite system to be used as a filler for flow resins. The cell viability decreases slightly with time after the treatment of L929 cells with modified resins leachate (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…This journal is © The Royal Society of Chemistry 2023 capacity. 7,9,23,24,31,[49][50][51][52][53][54][55] The absence of cytotoxicity is a necessary condition for the BNN/TiO 2 nanocomposite system to be used as a filler for flow resins. The cell viability decreases slightly with time after the treatment of L929 cells with modified resins leachate (Fig.…”

Section: Biomaterials Science Papermentioning

confidence: 99%

BNN/TiO₂ nanocomposite system–modified dental flow resins and the mechanism of the enhancement of mechanical and antibacterial properties

et al. 2023

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“…To develop the new metal methacrylate modified PMMA resin, ZDMA was added at 1 wt%, 2.5 wt%, and 5 wt% to the PMMA monomer. Mass fraction greater than 5 wt% of ZDMA were eliminated considering that the preliminary study ( An et al., 2022 ) has indicated a significant decrease in mechanical properties. Therefore, PMMA modified with ZDMA (1 wt%, 2.5 wt% and 5 wt%) were applied for the experimental groups, and unmodified PMMA for the control.…”

Section: Methodsmentioning

confidence: 99%

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

Song

Zhao

et al. 2023

Front. Cell. Infect. Microbiol.

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ObjectivesConsidering the high incidence rates of denture stomatitis, research that providing dental biomaterials with antifungal property are essential for clinical dentistry. The objectives of the present study were to investigate the effect of zinc dimethacrylate (ZDMA) modification on the antifungal and cytotoxic properties, as well as the variance in surface characteristics and other physicochemical properties of polymethyl methacrylate (PMMA) denture base resin.MethodsPMMA with various mass fraction of ZDMA (1 wt%, 2.5 wt% and 5 wt%) were prepared for experimental groups, and unmodified PMMA for the control. Fourier-transform infrared spectroscopy (FTIR) was applied for characterization. Thermogravimetric analysis, atomic force microscopy and water contact angle were performed to investigate the thermal stability and surface characteristics (n=5). Antifungal capacities and cytocompatibility were evaluated with Candida albicans (C. albicans) and human oral fibroblasts (HGFs), respectively. Colony-forming unit counting, crystal violet assay, live/dead biofilm staining and scanning electron microscopy observation were performed to assess antifungal effects, and the detection of intracellular reactive oxygen species production was applied to explore the possible antimicrobial mechanism. Finally, the cytotoxicity of ZDMA modified PMMA resin was evaluated by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and live/dead double staining.ResultsThe FTIR analyses confirmed some variation in chemical bonding and physical blend of the composites. Incorporation of ZDMA significantly enhanced the thermal stability and hydrophilicity compared with unmodified PMMA (p < 0.05). The surface roughness increased with the addition of ZDMA while remained below the suggested threshold (≤ 0.2 µm). The antifungal activity significantly improved with ZDMA incorporation, and cytocompatibility assays indicated no obvious cytotoxicity on HGFs.ConclusionsIn the present study, the ZDMA mass fraction up to 5 wt% in PMMA performed better thermal stability, and an increase in surface roughness and hydrophilicity without enhancing microbial adhesion. Moreover, the ZDMA modified PMMA showed effective antifungal activity without inducing any cellular side effects.

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Mechanical and antibacterial properties of polymethyl methacrylate modified with zinc dimethacrylate

Cited by 16 publications

References 32 publications

Ultrasonic-assisted extraction, anti-biofilm activity, and mechanism of action of Ku Shen (Sophorae Flavescentis Radix) extracts against Vibrio parahaemolyticus

Ultrasonic-assisted extraction, anti-biofilm activity, and mechanism of action of Ku Shen (Sophorae Flavescentis Radix) extracts against Vibrio parahaemolyticus

BNN/TiO₂ nanocomposite system–modified dental flow resins and the mechanism of the enhancement of mechanical and antibacterial properties

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

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Mechanical and antibacterial properties of polymethyl methacrylate modified with zinc dimethacrylate

Cited by 16 publications

References 32 publications

Ultrasonic-assisted extraction, anti-biofilm activity, and mechanism of action of Ku Shen (Sophorae Flavescentis Radix) extracts against Vibrio parahaemolyticus

Ultrasonic-assisted extraction, anti-biofilm activity, and mechanism of action of Ku Shen (Sophorae Flavescentis Radix) extracts against Vibrio parahaemolyticus

BNN/TiO2 nanocomposite system–modified dental flow resins and the mechanism of the enhancement of mechanical and antibacterial properties

Antifungal efficiency and cytocompatibility of polymethyl methacrylate modified with zinc dimethacrylate

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BNN/TiO₂ nanocomposite system–modified dental flow resins and the mechanism of the enhancement of mechanical and antibacterial properties