Measuring tryptophan dynamics using fast scan cyclic voltammetry at carbon fiber microelectrodes with improved sensitivity and selectivity

Abstract

Here we report an optimized sawhorse waveform in fast scan cyclic voltammetry for the sensitive and selective detection of tryptophan (Trp) at carbon fiber microelectrodes and demonstrate the utility of this method in measuring Trp dynamics in different cell lines.

Fabrication and Optimization of a Molecularly Imprinted Carbon Fiber Microelectrode for Selective Detection of Met-enkephalin Using Fast-Scan Cyclic Voltammetry

“…Applying a high positive potential (1.3+ V) strips the topmost layer of CFMs, thereby allowing for renewal of the electrode surface between recordings, prevents biofouling, and permits continuous monitoring . FSCV recordings at CFMs are stable over numerous consecutive measurements, even with coatings attached to the surface, as shown by us and others. ,, Additionally, the highly adsorptive nature of carbon allows for preconcentration of analytes, making this method highly sensitive (with nM limit of detection) . The only limitation of this method is its specificity.…”

“…43 FSCV recordings at CFMs are stable over numerous consecutive measurements, even with coatings attached to the surface, as shown by us and others. 38,44,45 Additionally, the highly adsorptive nature of carbon allows for preconcentration of analytes, making this method highly sensitive (with nM limit of detection). 46 The only limitation of this method is its specificity.…”

An Updated Review on Electrochemical Nanobiosensors for Neurotransmitter Detection

“…Through the utilization of current measurements, researchers are able to acquire valuable information related to several biological processes, including, but not limited to, neurotransmitter release and uptake [ 66 ]. The utilization of this technique has demonstrated significant value in the field of neuroscience research, providing valuable insights into fundamental elements of brain functioning regarding addiction and several neurological disorders [ 67 , 68 ]. Moreover, FSCV has been utilized in several fields, such as environmental monitoring, medicinal research, and materials science, wherein the comprehension of fast electrochemical phenomena holds substantial importance [ 69 , 70 ].…”

Waveform Optimization for the In Vitro Detection of Caffeic Acid by Fast-Scan Cyclic Voltammetry

“…Fast-scan cyclic voltammetry (FSCV) is an electroanalytical technique that uses voltage sweeps with scan rates above 100 V/s . An advantage of FSCV over other approaches is that the applied waveform can be applied successively at high frequencies, allowing users to monitor changes in electroactive biomolecules on a subsecond time scale, while maintaining the ability to identify the analyte of interest based on the shapes of the recorded cyclic voltammograms (CV). , Coupled with small-sized biocompatible carbon fiber microelectrodes (CFMs), this technique has been used successfully for over 40 years for the real-time detection of neurochemicals in the living brain, primarily catecholamines. , More recent FSCV advances have included novel waveforms, CFM modifications for enhanced detection capabilities, and an expansion in the types of analytes detected including amino acids, metal ions, metabolites, peptides, hormones, and other small molecules of biochemical significance. − …”