Abstract:Calling cards technology using self-reporting transposons enables the identification of DNA-protein interactions through RNA sequencing. By introducing a DNA barcode into the calling card itself, we have drastically reduced the cost and labor requirements of calling card experiments in bulk populations of cells. An additional barcode incorporation during reverse transcription enables simultaneous transcriptome measurement in a facile and affordable protocol. We demonstrate that barcoded self-reporting transpos… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.