Measuring the microelastic properties of biological material

Tao, Nongjian; Lindsay, Stuart; Lees, Sidney

doi:10.1016/s0006-3495(92)81692-2

Cited by 205 publications

(119 citation statements)

References 14 publications

(21 reference statements)

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“…The elastic properties of bone and bone marrow 35) and gelatin 36) have been determined by AFM. Determination of surface hardness can be most easily performed by obtaining force curves while the tip is raster-scanned across the sample 37,38) .…”

Section: Discussionmentioning

confidence: 99%

Atomic force microscopy observation of enamel surfaces treated with selfetching primer

Hashimoto

Nishiura

et al. 2013

Dent. Mater. J.

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Orthodontists use a self-etching adhesive system when attaching brackets to enamel. The purpose of this study was to evaluate the erosion effects of common clinically used adhesive systems on human enamel surfaces by atomic force microscopy (AFM). Four commercially available adhesive systems (i.e., Kurasper F, Beauty Ortho Bond, Orthophia LC, and Transbond XT) were applied to ground enamel surfaces of extracted human teeth. Enamel surface roughness (ESR), absolute depth profile (ADP), and surface hardness were evaluated by AFM. The ESR and ADP were significantly higher after the pretreatment with the phosphoric acidetching adhesive system than after the pretreatments with the three self-etching adhesive systems. The surface nanohardness decreased after the pretreatment with the phosphoric acid-etching adhesive system but increased after the pretreatments with the self-etching adhesive systems. These results suggest that the use of a self-etching primer for enamel conditioning might prevent decalcification caused by phosphoric acid etching.

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Section: Discussionmentioning

confidence: 99%

Atomic force microscopy observation of enamel surfaces treated with selfetching primer

Hashimoto

Nishiura

et al. 2013

Dent. Mater. J.

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“…Three types of mechanical loading on cells can be categorized (Bao and Suresh, 2003), and each serves a different purpose. Atomic force microscopy (AFM) and magnetic twisting cytometry (MTC) are local probes that induce deformation in a portion of a cell and may be used in quantifying local cellular mechanical properties (Tao et al, 1992;Wang and Ingber, 1995). Micropipette aspiration and optical trapping exert mechanical loading on a whole cell and are useful for determining the mechanical properties of the entire cell (Hochmuth, 2000;Takahashi et al, 2003).…”

Section: Fibroblasts and Mechanical Loadsmentioning

confidence: 99%

Mechanoregulation of gene expression in fibroblasts

Wang

Thampatty

Lin

et al. 2007

Gene

219

187

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Mechanical loads placed on connective tissues alter gene expression in fibroblasts through mechanotransduction mechanisms by which cells convert mechanical signals into cellular biological events, such as gene expression of extracellular matrix components (e.g., collagen). This mechanical regulation of ECM gene expression affords maintenance of connective tissue homeostasis. However, mechanical loads can also interfere with homeostatic cellular gene expression and consequently cause the pathogenesis of connective tissue diseases such as tendinopathy and osteoarthritis. Therefore, the regulation of gene expression by mechanical loads is closely related to connective tissue physiology and pathology. This article reviews the effects of various mechanical loading conditions on gene regulation in fibroblasts and discusses several mechanotransduction mechanisms. Future research directions in mechanoregulation of gene expression are also suggested.

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“…For instance, the AFM based imaging, manipulation, and property characterization of biological molecules in liquids are a rapidly growing field. [1][2][3][4] The imaging resolution and force exerted on the sample are directly correlated to the quality factor of the AFM microcantilever in the surrounding liquid. 5 Any improvement in the microcantilever quality factor through mechanical design would be a significant advance in this area.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic loading of microcantilevers vibrating in viscous fluids

Basak

Raman

Garimella

2006

Journal of Applied Physics

208

179

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The hydrodynamic loading of elastic microcantilevers vibrating in viscous fluids is analyzed computationally using a three-dimensional, finite element fluid-structure interaction model. The quality factors and added mass coefficients of several modes are computed accurately from the transient oscillations of the microcantilever in the fluid. The effects of microcantilever geometry, operation in higher bending modes, and orientation and proximity to a surface are analyzed in detail. The results indicate that in an infinite medium, microcantilever damping arises from localized fluid shear near the edges of the microcantilever. Closer to the surface, however, the damping arises due to a combination of squeeze film effects and viscous shear near the edges. The dependence of these mechanisms on microcantilever geometry and orientation in the proximity of a surface are discussed. The results provide a comprehensive understanding of the hydrodynamic loading of microcantilevers in viscous fluids and are expected to be of immediate interest in atomic force microscopy and microcantilever biosensors.

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Measuring the microelastic properties of biological material

Cited by 205 publications

References 14 publications

Atomic force microscopy observation of enamel surfaces treated with selfetching primer

Atomic force microscopy observation of enamel surfaces treated with selfetching primer

Mechanoregulation of gene expression in fibroblasts

Hydrodynamic loading of microcantilevers vibrating in viscous fluids

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