Abstract:Mechanical properties of cells and extracellular matrix (ECM) play important roles in many biological processes including stem cell differentiation, tumor formation, and wound healing. Changes in stiffness of cells and ECM are often signs of changes in cell physiology or diseases in tissues. Hence, cell stiffness is an index to evaluate the status of cell cultures. Among the multitude of methods applied to measure the stiffness of cells and tissues, micro-indentation using an Atomic Force Microscope (AFM) prov… Show more
“…AFM based microrheology measurements [16,17] were also used to characterize the local viscoelasticity of polyacrylamide gel. In this work, AFM was used to serve as a reference to verify the inclined DFOTs measurements.…”
Section: Experimental Results Measured By Afmmentioning
confidence: 99%
“…In this work, AFM [16,17] was used to characterize local viscoelastic properties of polyacrylamide gel around the bead that was studied in the inclined DFOTs. An Asylum Research MFP3D-BIO AFM (Asylum Research, CA) was used for the measurements.…”
Section: Afm Measurements Of Polyacrylamide Gel Modulimentioning
confidence: 99%
“…Generally, local mechanical properties of the substrates, such as polyacrylamide gel, is measured by using atomic force microscopy (AFM) [16,17]. However, the AFM based microrheology measurements require physical contact, and the resolution of lateral force measurements is limited.…”
We developed an inclined dual fiber optical tweezers (DFOTs) for simultaneous force application and measurements in a 3D hydrogel matrix. The inclined DFOTs provide a potential solution for cell mechanics study in a three-dimensional matrix.
“…AFM based microrheology measurements [16,17] were also used to characterize the local viscoelasticity of polyacrylamide gel. In this work, AFM was used to serve as a reference to verify the inclined DFOTs measurements.…”
Section: Experimental Results Measured By Afmmentioning
confidence: 99%
“…In this work, AFM [16,17] was used to characterize local viscoelastic properties of polyacrylamide gel around the bead that was studied in the inclined DFOTs. An Asylum Research MFP3D-BIO AFM (Asylum Research, CA) was used for the measurements.…”
Section: Afm Measurements Of Polyacrylamide Gel Modulimentioning
confidence: 99%
“…Generally, local mechanical properties of the substrates, such as polyacrylamide gel, is measured by using atomic force microscopy (AFM) [16,17]. However, the AFM based microrheology measurements require physical contact, and the resolution of lateral force measurements is limited.…”
We developed an inclined dual fiber optical tweezers (DFOTs) for simultaneous force application and measurements in a 3D hydrogel matrix. The inclined DFOTs provide a potential solution for cell mechanics study in a three-dimensional matrix.
“…These findings should be further validated in additional experiments, including more mice, corroborative tests, and possibly by using techniques such as atomic force microscopy. 37,38 The effect of treatment duration should also be evaluated. It should also be noted that the calculated values of ΔT functional are low when compared to the previous research.…”
“…It uses a flexible cantilever with a fine tip at its free end, to probe cellular structures [5][6]. The tip is directed downward by a piezoelectric stage to probe a sample.…”
Section: A Atomic Force Microscope (Afm)mentioning
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