Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

Sousa, Tânia; Castro, Rui E.; Coutinho, Ana; Rodrigues, Cecília M. P.; Prieto, Manuel; Fernandes, Fábio

doi:10.1007/978-1-4939-9420-5_7

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…However, to determine whether this cytotoxicity is mediated through mitochondrial dysfunction, studies have been conducted on the liver's mitochondrial function. These studies are crucial in understanding the harmful effects of BAs and the mechanisms through which they cause damage (Sousa et al 2019). The build-up of BAs also negatively affects the activities of mitochondrial enzyme complexes and the energetic metabolism.…”

Section: Altered Levels Of Bile Acids Carnitine Fatty Acids and Stero...mentioning

confidence: 99%

Metabolomics-based Investigation of Primary Biliary Cholangitis: A Cholestatic Liver Disease

de Oliveira,

Lopes,

Castro

et al. 2024

Preprint

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Introduction Primary Biliary Cholangitis (PBC) is a rare disease that affects the liver. It causes the progressive destruction of the intrahepatic bile ducts, leading to liver fibrosis. Currently, the diagnosis of PBC includes a medical and family history, physical exams, blood tests, imaging tests, and occasionally a liver biopsy. If not promptly treated, PBC progresses to cirrhosis, liver failure, and death. Objectives To improve the development of new diagnostic or prognostic methods for PBC, a metabolomic-based study was conducted to evaluate the metabolomic profiles reflected in plasma and urine samples from healthy individuals and PBC patients. This study aimed to gain a better understanding of the underlying pathological mechanisms of PBC. Methods Blood plasma and urine samples were collected from 30 female PBC patients and 20 female healthy controls. The study used an untargeted metabolomic approach involving liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). The data was processed using multivariate and univariate statistical methods. Results Forty-seven plasma and fifty-six urine metabolites demonstrated statistical differences between PBC patients and healthy controls (p ≤ 0.05). The most significant differences were found in metabolites related to bile acid and lipid metabolism (including phospholipids and fatty acids) and branched-chain amino acids. These findings indicate that metabolomic profiling in plasma and urine can help identify new diagnostic biomarkers for PBC. Conclusions The study highlights metabolites linked to fatty acid beta-oxidation, bile acid biosynthesis, and amino acid metabolism as potential candidates for biomarkers in PBC, which can assist further studies for PBC diagnosis and therapeutic monitoring.

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Section: Altered Levels Of Bile Acids Carnitine Fatty Acids and Stero...mentioning

confidence: 99%

Metabolomics-based Investigation of Primary Biliary Cholangitis: A Cholestatic Liver Disease

de Oliveira,

Lopes,

Castro

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…With similar fractionation strategies, bottom‐up studies employed offline high‐pH RPLC to screen digested peptides from a variety of biological samples. Between these samples are pancreas cancer tissue [136], prostate cancer tissue [137], bronchoalveolar lavage fluid for lung cancer biomarker research [138], Trypanosoma brucei cells [139], primary biliary cholangitis from human serum [140], Panax ginseng cauline leaf and root [141], Saccharomyces cerevisiae yeast [142], and HeLa cells [143–145].…”

Section: High‐ph Rplcmentioning

confidence: 99%

Recent stationary phase‐based fractionation strategies in proteomic analysis

Ribeiro

Urban

2023

J of Separation Science

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In this article, we review stationary phase‐assisted sample prefractionation in proteomic analysis in 2019–2022 period. Applications are grouped according to the mode of retention used in the prefractionation step prior to the final analysis by low‐pH reversed‐phase liquid chromatography‐tandem mass spectrometry. Online or offline instrumental configurations are also commented on, with a highlight on novel online platforms. From the articles reviewed in this period, the order of popularity of chromatographic modes for sample fractionation is affinity chromatography > size exclusion chromatography > hydrophilic interaction chromatography > high‐pH reversed‐phase liquid chromatography > ion exchange chromatography.

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A new minimally invasive porcine model for the study of intrahepatic bile duct dilatation

et al. 2020

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Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

Cited by 4 publications

References 38 publications

Metabolomics-based Investigation of Primary Biliary Cholangitis: A Cholestatic Liver Disease

Metabolomics-based Investigation of Primary Biliary Cholangitis: A Cholestatic Liver Disease

Recent stationary phase‐based fractionation strategies in proteomic analysis

A new minimally invasive porcine model for the study of intrahepatic bile duct dilatation

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