Measuring the immune system: a comprehensive approach for the analysis of immune functions in humans

Claus, Maren; Dychus, Nicole; Ebel, Melanie; Damaschke, Jürgen; Maydych, Viktoriya; Wolf, Oliver T.; Kleinsorge, Thomas; Watzl, Carsten

doi:10.1007/s00204-016-1809-5

Cited by 24 publications

(15 citation statements)

References 68 publications

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“…To investigate changes of the immune system in preparation for, during, and after this stressful exam period we examined the students at five different sessions over a period of 8 weeks ( Fig 1 ). In addition to a psychometric test battery, we took saliva samples to measure cortisol levels and blood samples to determine immunological parameters [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…Immunological phenotyping was performed using blood samples as recently described [ 38 ]. Using the methods described in this publication we determined the absolute number of T cells, B cells, NK cells and monocytes in whole blood.…”

Section: Methodsmentioning

confidence: 99%

“…Finally, we determined the concentrations of cytokines in serum samples. The biological functions of the different immune parameters investigated in this study are detailed in a recent publication [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

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Impact of chronic and acute academic stress on lymphocyte subsets and monocyte function

et al. 2017

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This study investigated the effects of a temporally confined naturalistic stressor (academic stress) on immune functions. Furthermore, moderating influences of a number of psychological variables were assessed. Five blood samples were obtained from 20 students during an observation period of 8 weeks, starting 4.5 weeks before an exam period up to 1 week following the last exam. The analysis of 45 immune parameters revealed several time-dependent changes attributable to examination stress. We observed a reduction in the absolute numbers of natural killer (NK) cells and monocytes in peripheral blood and a shift towards more immature and naïve cells within NK and T cell populations. In addition, IL-6 and TNF-α production by LPS-stimulated monocytes was increased. Psychological variables were grouped by means of factor analyses into two factors. One factor, which was interpreted as an indication of chronic stress, moderated the relationships between academic stress and percentages of mature CD57+ NK cells. This chronic stress factor was also associated with an increase in memory and a decrease in naïve CD8 T cells and increased serum levels of IL-17. The present study identifies important potential psychological mediators of stress-induced changes in specific immunological parameters.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Impact of chronic and acute academic stress on lymphocyte subsets and monocyte function

et al. 2017

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“…Cytotoxic capacity was determined by a standard 4-h chromium release assay as described previously (25). In brief, 5 3 10 5 target cells were labeled in 100 ml medium with 100 mCi [ 51 Cr] (Hartmann Analytic) for 1 h at 37˚C, 5% CO 2 .…”

Section: Chromium Release Assaymentioning

confidence: 99%

Termination of the Activating NK Cell Immunological Synapse Is an Active and Regulated Process

Netter

Anft

Watzl

2017

The Journal of Immunology

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Cellular cytotoxicity is essential for the elimination of virus-infected and cancerous cells by NK cells. It requires a direct cellular contact through the establishment of an immunological synapse (IS) between the NK cell and the target cell. In this article, we show that not only the establishment of the IS, but also its maintenance is a highly regulated process. Ongoing receptor-proximal signaling events from activating NK cell receptors and actin dynamics were necessary to maintain a stable contact in an energy-dependent fashion, even after the IS was formed successfully. More importantly, the initiation of a contact to a new susceptible target cell resulted in accelerated detachment from an old target cell. We propose that the maintenance of an existing IS is a dynamic and regulated process to allow for effective serial killing of NK cells.

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“…In mouse models, inhibition of the inflammatory microenvironment within tumors has been implicated as a therapeutic target for inflammationassociated cancers such as HCC. [17][18][19] In addition, tumorous angiogenesis was promoted by cytokines in inflammation, 15,16 and microvessel density was found positively associated with the presence of macrophages. 16 Furthermore, cytokines derived from macrophage-regulated inflammation are known to also contribute to cancer progression by promoting angiogenesis.…”

Section: Introductionmentioning

confidence: 95%

<p>Hydrocortisone Suppresses Early Paraneoplastic Inflammation And Angiogenesis To Attenuate Early Hepatocellular Carcinoma Progression In Rats</p>

Liu¹,

Cui²,

Niu³

et al. 2019

OTT

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Background: Inflammation is implicated in both hepatic cirrhosis development and hepatocellular carcinogenesis, and treatment with long-acting glucocorticoid dexamethasone prevented liver carcinogenesis in mice. However, it is unclear whether glucocorticoids have anti-inflammatory effect on hepatocellular carcinoma (HCC) and if short-acting glucocorticoids (with fewer adverse effects) inhibit paraneoplastic inflammation and HCC progression. Methods: To investigate whether different types of anti-inflammatory agents attenuate HCC progression, the current study compared effects of treatments with hydrocortisone (a shortacting glucocorticoid) or aspirin on HCC progression. HCC was induced in diethylnitrosaminetreated rats which were randomly divided into 4 groups (n=8), respectively receiving orally once daily vehicle, glucuronolactone, glucuronolactone+hydrocortisone, and glucuronolactone +aspirin. Diethylnitrosamine (DEN) was given to rats in drinking water (100mg/L) to induce HCC. At weeks 12 and 16 post-induction, effects were compared on HCC nodule formation, microvessel density, and macrophage infiltration, and levels of paraneoplastic protein expression of tumor necrosis factor (TNF)-α, p38 mitogen-activated protein kinase (p38), phosphorylated p38 (p-p38), nuclear factor (NF)-κB, interleukin (IL)-10, hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1 and vascular endothelial growth factor (VEGF). Results: Compared to the model and glucuronolactone alone groups, HCC nodule number and microvessel density in the glucuronolactone+hydrocortisone group were significantly lower at week 12. At week 12 but not week 16, significantly lower levels of macrophages, TNF-α, p-p38, NF-κB, IL-10, HGF, TGF-β1 and VEGF were observed in the paraneoplastic tissue of the glucuronolactone+hydrocortisone group when compared with the control and glucuronolactone groups. Conclusion: The results suggest that hydrocortisone treatment reduces macrophage polarization, expression of inflammatory and anti-inflammatory cytokines, and angiogenesis in paraneoplastic tissue, and attenuates early HCC progression. Although hydrocortisone did not have attenuation effect on advanced solid tumor, the current study shows the potential benefits and supports potential clinical use of hydrocortisone in attenuating early progression of HCC, which is through suppressing paraneoplastic inflammation and angiogenesis.

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Measuring the immune system: a comprehensive approach for the analysis of immune functions in humans

Cited by 24 publications

References 68 publications

Impact of chronic and acute academic stress on lymphocyte subsets and monocyte function

Impact of chronic and acute academic stress on lymphocyte subsets and monocyte function

Termination of the Activating NK Cell Immunological Synapse Is an Active and Regulated Process

<p>Hydrocortisone Suppresses Early Paraneoplastic Inflammation And Angiogenesis To Attenuate Early Hepatocellular Carcinoma Progression In Rats</p>

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