Measuring rheumatoid factor in nonrheumatoid subjects: immunoturbidimetric assay, latex slide test, and enzyme-linked immunosorbent assay compared

Ailus, Kirsi; Melamies, L.; Tuomi, Tiinamaija; Palosuo, T; K, Aho

doi:10.1093/clinchem/37.10.1766

Cited by 14 publications

(6 citation statements)

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“…Automated techniques such as nephelometry and enzyme-linked immunosorbent assays gradually replaced the other semiquantitative methods because of their simplicity and greater reproducibility [ 9 – 12 ].…”

Section: Methods Of Detectionmentioning

confidence: 99%

Rheumatoid Factors: Clinical Applications

2013

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Rheumatoid factors are antibodies directed against the Fc region of immunoglobulin G. First detected in patients with rheumatoid arthritis 70 years ago, they can also be found in patients with other autoimmune and nonautoimmune conditions, as well as in healthy subjects. Rheumatoid factors form part of the workup for the differential diagnosis of arthropathies. In clinical practice, it is recommended to measure anti-cyclic citrullinated peptide antibodies and rheumatoid factors together because anti-cyclic citrullinated peptide antibodies alone are only moderately sensitive, and the combination of the two markers improves diagnostic accuracy, especially in the case of early rheumatoid arthritis. Furthermore, different rheumatoid factor isotypes alone or in combination can be helpful when managing rheumatoid arthritis patients, from the time of diagnosis until deciding on the choice of therapeutic strategy.

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Section: Methods Of Detectionmentioning

confidence: 99%

Rheumatoid Factors: Clinical Applications

2013

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“…RF are still the most widely used serological test as an aid for the diagnosis of RA. Most routine laboratory tests in current use are the hemagglutination and the latex agglutination, which detected mainly IgM‐RF 12,13. In these techniques, end points are not objectively determined because an observer decides when agglutination is present and this judgment will vary among observers.…”

Section: Discussionmentioning

confidence: 99%

Multiplexed Immunoassay for Detection of Rheumatoid Factors by FIDIS™ Technology

Abreu

Laroche²,

Bastos

et al. 2005

Annals of the New York Academy of Sciences

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Our objective was to (i) compare FIDIS Rheuma, a new multiplexed immunoassay designed for simultaneous detection of IgM class rheumatoid factors (RF) directed against Fc determinants of IgG from humans and animals, with agglutination and ELISA (conventional methods) and (ii) evaluate the clinical sensitivity and specificity of biological markers for rheumatoid arthritis (RA). To do this, FIDIS technology was employed using the Luminex system. It consists of distinct color-coded microsphere sets, a flow cytometer, and digital signal processing hardware and software. Agglutination and ELISA tests were performed with commercial kits. The study included 134 samples from RA patients and 105 from healthy blood donors. For human specificity, we compared FIDIS with latex agglutination and ELISA. Relative sensitivities were 98.9% and 88.5% and specificities were 90.2% and 94.6%, respectively. For animal specificity, we compared FIDIS with Waaler-Rose and ELISA. The results were 84.9% and 71.9% for the sensitivities and 97.5% and 98.4% for the specificities, respectively. Detection of IgG anti-CCP by ELISA and IgG antikeratin by immunofluorescence was also determined in order to compare their clinical sensitivity and specificity with IgM-RF, according to the method used. The results were: IgG anti-CCP 72.3%, 97.2%; IgG antikeratin 36.6%, 100%; latex agglutination 66.4%, 97.2%; Waaler-Rose 55.9%, 96.3%; FIDIS human 73.9%, 92.1%; FIDIS animal 49.2%, 97.2%; ELISA human 93.2%, 95.5%; and ELISA animal 74.6%, 91.3%. The results showed the efficiency of FIDIS with analytical performance equivalent to the conventional methods, but having the advantage of giving quantitative results (IU/mL).

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“…An ESR >20 mm/h was considered positive (18). Rheumatoid factor (RF) and C-reactive protein (CRP) were measured by immunonephelometry, RF >15 U/ml and CRP >8 mg/l were considered positive (19,20). Anti-cyclic citrullinated peptide (anti-CCP) antibody levels were measured by chemiluminescence microparticle immunoassay, anti-CCP titers <5.0 RU/ml were judged as negative, and ≥5.0 RU/ml as positive (21).…”

Section: Methodsmentioning

confidence: 99%

Differential long non‑coding RNA expression profiles in the peripheral blood and CD4+ T cells of patients with active rheumatoid arthritis

Feng

et al. 2020

Exp Ther Med

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The human transcriptome is primarily composed of long non-coding RNAs (lncRNAs), which are key regulatory molecules of multiple biological processes. In the present study, the expression profiles of lncRNAs in the peripheral blood and CD4 + T cells of patients with active rheumatoid arthritis (RA) were determined. Based on the expression profiles, 493 lncRNAs and 374 mRNAs were identified to be differentially expressed in the peripheral blood of active RA patients and healthy donors. Further verification of lncRNAs was performed using reverse transcription-quantitative (RT-q) PCR analysis of peripheral blood from 5 healthy donors and 5 patients with active RA and 14 additional differentially expressed genes were identified. CD4 + T cells in peripheral blood from 12 patients with active RA and 8 healthy donors were isolated using magnetic beads and qPCR was used to assess differentially expressed lncRNAs. The results suggested that 7 lncRNAs were upregulated and 2 were downregulated. The results indicated that these 9 lncRNAs may be involved in the pathogenesis of RA. An increased ratio of Th17: T-regulatory (Treg) cells was also observed. It may be hypothesized that LncRNAs serve important roles in the differentiation of CD4 + T cells. Receiver operating characteristic curve analysis suggested that these 9 lncRNAs are of potential clinical diagnostic value for RA. Pearson correlation analysis indicated that the correlation coefficient between Ensembl transcript (ENST)00000569543 and complement C4 was 0.623 (P<0.05), and that between ENST00000420096 and anti-cyclic citrullinated peptide antibody or disease activity evaluation score, the correlation coefficient was 0.662 and 0.605, respectively (P<0.05 for each). In conclusion, the results of the present study suggest a possible role of lncRNAs in the differentiation of CD4 + T cells and the pathogenesis of RA, as well as the potential value as diagnostic biomarkers for active RA.

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Measuring rheumatoid factor in nonrheumatoid subjects: immunoturbidimetric assay, latex slide test, and enzyme-linked immunosorbent assay compared

Cited by 14 publications

References 0 publications

Rheumatoid Factors: Clinical Applications

Rheumatoid Factors: Clinical Applications

Multiplexed Immunoassay for Detection of Rheumatoid Factors by FIDIS™ Technology

Differential long non‑coding RNA expression profiles in the peripheral blood and CD4+ T cells of patients with active rheumatoid arthritis

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