Measuring piecemeal microautophagy of the nucleus in<i>Saccharomyces cerevisiae</i>

Millen, Jonathan; Krick, Roswitha; Prick, Tanja; Thumm, Michael; Goldfarb, David S.

doi:10.4161/auto.5.1.7181

Cited by 46 publications

(41 citation statements)

References 12 publications

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“…PMN is classified as microautophagy because the delivery of the nuclear bleb to the vacuole occurs by direct invagination and scission of the vacuole membrane, without involvement of autophagosome structures. Notably, components of the homotypic vacuole fusion machinery that are required for macroautophagy processes are not needed, emphasizing that PMN is a microautophagy process (Krick et al, 2008;Krick et al, 2009;Millen et al, 2009). As yeast has a closed mitosis (i.e.…”

Section: Piecemeal Microautophagy Of the Nucleusmentioning

confidence: 99%

“…The cargo that is sequestered by PMN includes nonessential nuclear components, such as the granular nucleolus that is enriched in preribosomes, as well as the surrounding portion of nuclear membranes (Roberts et al, 2003). Nucleus-derived components, such as bulk chromatin, nuclear pore complexes and spindle pole bodies are excluded from PMN vesicles (Roberts et al, 2003;Krick et al, 2009;Millen et al, 2009).…”

Section: Piecemeal Microautophagy Of the Nucleusmentioning

confidence: 99%

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Nucleophagy at a glance

Mijaljica

Devenish

2013

Journal of Cell Science

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Under certain circumstances, the removal of damaged or non-essential parts of the nucleus, or even an entire nucleus, is crucial in order to promote cell longevity and enable proper function. A selective form of autophagy, known as nucleophagy, can be used to accomplish the degradation of nucleusderived material. In this Cell Science at a Glance article and the accompanying poster, we summarize the similarities and differences between the divergent modes of nucleophagy that have been described to date, emphasizing, where possible, the molecular mechanism, the membrane interactions and rearrangements, and the nature of the nucleus-derived material that is degraded. In turn, we will consider nucleophagy processes in the lower eukaryotes, the budding yeast Saccharomyces cerevisiae, filamentous fungi Aspergillus and Magnaporthe oryzae and the ciliated protozoan Tetrahymena thermophila, and finally in mammalian cells. We will also briefly discuss the emerging links between nucleophagy and human disease.

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Section: Piecemeal Microautophagy Of the Nucleusmentioning

confidence: 99%

Section: Piecemeal Microautophagy Of the Nucleusmentioning

confidence: 99%

Nucleophagy at a glance

Mijaljica

Devenish

2013

Journal of Cell Science

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“…Therefore we tested weather Nvj2p was also required for PMN. Previous studies have shown that rapamycin induces PMN (Dawaliby and Mayer, 2010;Millen et al, 2009). To assess PMN induction we monitored the internalization of the nucleoplasm protein Pus1-GFP in the vacuole after rapamycin addition.…”

Section: Nvj2p Is Not Required For Nvj Formation or Piecemeal Microaumentioning

confidence: 99%

A conserved membrane-binding domain targets proteins to organelle contact sites

Toulmay

Prinz

2012

Journal of Cell Science

214

277

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SummaryMembrane contact sites (MCSs), where the membranes of two organelles are closely apposed, are regions where small molecules such as lipids or calcium are exchanged between organelles. We have identified a conserved membrane-binding domain found exclusively in proteins at MCSs in Saccharomyces cerevisiae. The synaptotagmin-like-mitochondrial-lipid binding protein (SMP) domain is conserved across species. We show that all seven proteins that contain this domain in yeast localize to one of three MCSs. Human proteins with SMP domains also localize to MCSs when expressed in yeast. The SMP domain binds membranes and is necessary for protein targeting to MCSs. Proteins containing this domain could be involved in lipid metabolism. This is the first protein domain found exclusively in proteins at MCSs.

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“…65 Nuclear components targeted for PMN include granular nucleolus enriched in pre-ribosomes and nuclear envelope, nuclear pore complexes or spindle pole bodies. 66,67 PMN involves the core Atg proteins involved in macroautophagy and microautophagy, such as Atg4, 5, 7 and 12, as well as macroautophagy-specific proteins such as Atg17, 29 and 31. 64 In mammalian cells, autophagy can degrade nuclear components, thereby contributing to the maintenance of nuclear function and integrity.…”

Section: Nucleophagy As a Way To Eliminate Injured Dnamentioning

confidence: 99%

Autophagy and genomic integrity

et al. 2013

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DNA lesions, constantly produced by endogenous and exogenous sources, activate the DNA damage response (DDR), which involves detection, signaling and repair of the damage. Autophagy, a lysosome-dependent degradation pathway that is activated by stressful situations such as starvation and oxidative stress, regulates cell fate after DNA damage and also has a pivotal role in the maintenance of nuclear and mitochondrial genomic integrity. Here, we review important evidence regarding the role played by autophagy in preventing genomic instability and tumorigenesis, as well as in micronuclei degradation. Several pathways governing autophagy activation after DNA injury and the influence of autophagy upon the processing of genomic lesions are also discussed herein. In this line, the mechanisms by which several proteins participate in both DDR and autophagy, and the importance of this crosstalk in cancer and neurodegeneration will be presented in an integrated fashion. At last, we present a hypothetical model of the role played by autophagy in dictating cell fate after genotoxic stress.

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Measuring piecemeal microautophagy of the nucleus inSaccharomyces cerevisiae

Cited by 46 publications

References 12 publications

Nucleophagy at a glance

Nucleophagy at a glance

A conserved membrane-binding domain targets proteins to organelle contact sites

Autophagy and genomic integrity

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