Measuring Physical Properties of Electrospun Nanofiber Mats for Different Biomedical Applications

Langwald, Sarah Vanessa; Ehrmann, Andrea; Sabantina, Lilia

doi:10.3390/membranes13050488

Cited by 19 publications

(18 citation statements)

References 176 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, upon compositing 1% gelatin in the 5% PCL polymer solution, the fibers started to form without any bead formation, as shown in Figure B–E. The pore size of the nanofiber mats (from n = 3 same magnification FESEM images) has been measured by employing the long-axis measurement method using ImageJ software, a similar protocol reported in the previous literature. , It was observed that with increasing gelatin content in the nanofiber mats, the pore size of the nanofiber mats decreased significantly, as shown in Figure F. Static collector-derived 5% PCL nanofiber mat reported pore size of 1.143 ± 0.069 μm, while 5% PCL + 1% gelatin composite nanofiber mats reported pore size of 0.750 ± 0.033 μm and 5% PCL + 4% gelatin nanofiber mat reported pore size of 0.516 ± 0.040 μm.…”

Section: Resultsmentioning

confidence: 53%

Unveiling Mechanobiology: A Compact Device for Uniaxial Mechanical Stimulation on Nanofiber Substrates and Its Impact on Cellular Behavior and Nanoparticle Distribution

Basak,

Tiwari,

Sharma

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Biotechnology and its allied sectors, such as tissue culture, regenerative medicine, and personalized medicine, primarily rely upon extensive studies on cellular behavior and their molecular pathways for generating essential knowledge and innovative strategies for human survival. Most such studies are performed on flat, adherent, plastic-based surfaces and use nanofiber and hydrogellike soft matrices from the past few decades. However, such static culture conditions cannot mimic the immediate cellular microenvironment, where they perceive or generate a myriad of different mechanical forces that substantially affect their downstream molecular pathways. Including such mechanical forces, still limited to specialized laboratories, using a few commercially available or noncommercial technologies are gathering increasing attention worldwide. However, large-scale consideration and adaptation by developing nations have yet to be achieved due to the lack of a cost-effective, reliable, and accessible solution. Moreover, investigations on cellular response upon uniaxial mechanical stretch cycles under more in vivo mimetic conditions are yet to be studied comprehensively. In order to tackle these obstacles, we have prepared a compact, 3D-printed device using a microcontroller, batteries, sensors, and a stepper motor assembly that operates wirelessly and provides cyclic mechanical attrition to any thin substrate. We have fabricated water-stable and stretchable nanofiber substrates with different fiber orientations by using the electrospinning technique to investigate the impact of mechanical stretch cycles on the morphology and orientation of C2C12 myoblast-like cells. Additionally, we have examined the uptake and distribution properties of BSA-epirubicin nanoparticles within cells under mechanical stimulation, which could act as fluorescently active drug-delivery agents for future therapeutic applications. Consequently, our research offers a comprehensive analysis of cellular behavior when cells are subjected to uniaxial stretching on various nanofiber mat architectures. Furthermore, we present a cost-effective alternative solution that addresses the long-standing requirement for a compact, user-friendly, and tunable device, enabling more insightful outcomes in mechanobiology.

show abstract

Section: Resultsmentioning

confidence: 53%

Unveiling Mechanobiology: A Compact Device for Uniaxial Mechanical Stimulation on Nanofiber Substrates and Its Impact on Cellular Behavior and Nanoparticle Distribution

Basak,

Tiwari,

Sharma

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…In this way, the program provides the area corresponding to the empty zones (porosity). Surface porosity was compare with a gravimetric method proposed by Safari et al [46,47]. To determine the weight of the enclosed water sample, the absorbed water at the sample surface was swiftly scraped using a paper filter and weighed to prevent evaporation.…”

Section: Pan Fibers Characterizationmentioning

confidence: 99%

Critical Electrospinning Parameters for Synthesis Control of Stabilized Polyacrylonitrile Nanofibers

Ruiz Rocha,

Moreno Tovar,

Navarro Mendoza

et al. 2023

Nanomaterials

View full text Add to dashboard Cite

Polyacrylonitrile (PAN) fibers are widely used as precursors in the manufacture of high-conducting and mechanically resistant carbon fibers. The modulation of such fibers is carried out through electrospinning. In this work, we show the production and control of the morphology of nanometric-range PAN fibers for their potential use as precursors for high-electrical-conductivity carbon fibers. PAN samples dissolved in dimethylformamide (DMF) were prepared at 6, 10, and 12% w/w, at 15 and 25 kV. The impact of the rotation of the collector drum at 100, 300, and 500 RPM was also studied. It was found that the percentage of PAN in the solution proportionally affects the diameter of the fibers and that the preparation potential affects the morphology. The rotation speed, when increased, decreases the diameter, and it has a negative impact on the morphology. Fibers prepared with 6% w/w at 15 kV and 500 RPM show 90 nm diameters, the smallest diameter of all the samples.

show abstract

“…5 Polycaprolactone-polytetrahydrofuran-polycaprolactone. 6 Polyethylene-co-vinyl acetate. 7 Polydimethylsiloxane.…”

Section: Solutions Using Block Copolymermentioning

confidence: 99%

“…Meanwhile, they are still imbued with the physical properties of the material from which they are built. These properties can help the materials to be applied in some applications that require a certain physical strength [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Structural Control of Nanofibers According to Electrospinning Process Conditions and Their Applications

Nguyen,

Roh,

Nguyen

et al. 2023

Micromachines

View full text Add to dashboard Cite

Nanofibers have gained much attention because of the large surface area they can provide. Thus, many fabrication methods that produce nanofiber materials have been proposed. Electrospinning is a spinning technique that can use an electric field to continuously and uniformly generate polymer and composite nanofibers. The structure of the electrospinning system can be modified, thus making changes to the structure, and also the alignment of nanofibers. Moreover, the nanofibers can also be treated, modifying the nanofiber structure. This paper thoroughly reviews the efforts to change the configuration of the electrospinning system and the effects of these configurations on the nanofibers. Excellent works in different fields of application that use electrospun nanofibers are also introduced. The studied materials functioned effectively in their application, thereby proving the potential for the future development of electrospinning nanofiber materials.

show abstract

Measuring Physical Properties of Electrospun Nanofiber Mats for Different Biomedical Applications

Cited by 19 publications

References 176 publications

Unveiling Mechanobiology: A Compact Device for Uniaxial Mechanical Stimulation on Nanofiber Substrates and Its Impact on Cellular Behavior and Nanoparticle Distribution

Unveiling Mechanobiology: A Compact Device for Uniaxial Mechanical Stimulation on Nanofiber Substrates and Its Impact on Cellular Behavior and Nanoparticle Distribution

Critical Electrospinning Parameters for Synthesis Control of Stabilized Polyacrylonitrile Nanofibers

Structural Control of Nanofibers According to Electrospinning Process Conditions and Their Applications

Contact Info

Product

Resources

About