Measuring Pattern Separation in Hippocampus by <i>in Situ</i> Hybridization

Eom, Kisang; Lee, Hyoung‐Ro

doi:10.1002/cpz1.522

Cited by 2 publications

(1 citation statement)

References 49 publications

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“…The fixed tissues were pretreated for protease digestion for 30 min at room temperature. The digested tissues were hybridized with appropriate H1a and Arc riboprobes according to instructions in manufacturer’s manual and previous studies and visualized to measure neural ensembles [ 21 , 28 ]. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Tocris bioscience, Bristol, UK).…”

Section: Methodsmentioning

confidence: 99%

Partial EC outputs by degraded cues are amplified in hippocampal CA3 circuits for retrieving stored patterns

Eom¹

2023

PLoS ONE

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Hippocampus is known to be important for episodic memories. Measuring of hippocampal neural ensembles is therefore important for observing hippocampal cognitive processes such as pattern completion. Previous studies on pattern completion had a limitation because the activities of CA3 were not simultaneously observed with the activities of the entorhinal cortex that project to the CA3. In addition, in previous research and modelling, distinct concepts such as pattern completion and pattern convergence have not been considered separately. Here, I used a molecular analysis technique that enables comparison of neural ensembles that evoked two successive events and evaluated neural ensembles in the hippocampal CA3 region and entorhinal cortex. By comparing neural ensembles in hippocampus and entorhinal cortex, I could obtain evidence that suggests pattern completion occurring in the CA3 region was induced by the partial input from EC. Use of the molecular-based ensemble measurement allows measuring two or more brain regions simultaneously, which can lead to insights into the cognitive functions of neural circuits.

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Section: Methodsmentioning

confidence: 99%

Partial EC outputs by degraded cues are amplified in hippocampal CA3 circuits for retrieving stored patterns

Eom¹

2023

PLoS ONE

Self Cite

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Sleep disturbance in Angelman syndrome patients

Qu,

Wang,

Guan

et al. 2024

Orphanet J Rare Dis

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Angelman syndrome (AS) is a neurodevelopmental disorder caused by abnormal expression of the maternal ubiquitin protein ligase E3A gene (UBE3A). As one of the most challenging symptoms and important focuses of new treatment, sleep disturbance is reported to occur in 70–80% of patients with AS and has a serious impact on the lives of patients and their families. Although clinical studies and animal model studies have provided some clues, recent research into sleep disorders in the context of AS is still very limited. It is generally accepted that there is an interaction between neurodevelopment and sleep; however, there is no recognized mechanism for sleep disorders in AS patients. Accordingly, there are no aetiologically specific clinical treatments for AS-related sleep disorders. The most common approaches involve ameliorating symptoms through methods such as behavioural therapy and symptomatic pharmacotherapy. In recent years, preclinical and clinical studies on the targeted treatment of AS have emerged. Although precision therapy for restoring the UBE3A level and the function of its signalling pathways is inevitably hindered by many remaining obstacles, this approach has the potential to address AS-related sleep disturbance.

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Measuring Pattern Separation in Hippocampus by in Situ Hybridization

Cited by 2 publications

References 49 publications

Partial EC outputs by degraded cues are amplified in hippocampal CA3 circuits for retrieving stored patterns

Partial EC outputs by degraded cues are amplified in hippocampal CA3 circuits for retrieving stored patterns

Sleep disturbance in Angelman syndrome patients

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