Measuring nerve growth factor in saliva by immunoassay: A cautionary note

Matin, Marla J.; Li, Da-Ming; Peterson, J C; Taylor, Marcus K.; Laurent, Heidemarie K.; Lucas, Todd; Granger, Steve; Granger, Steve W.

doi:10.1016/j.psyneuen.2015.09.030

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Nonetheless, just because an analyte can be measured in oral fluid does not necessarily mean that it should be measured, or that the interpretation of variation in that analyte's levels in saliva will be straightforward or meaningful. Our experience reveals the importance of approaching these advances with cautious enthusiasm [71,72]. Taken together, the present findings move us more toward enthusiasm than caution by suggesting moderate-to-strong measurement validity and stability when UA is measured in saliva, and evidence suggesting salivary UA may be a robust indicator of systemic UA activity.…”

Section: Resultssupporting

confidence: 55%

The Validity, Stability, and Utility of Measuring Uric Acid in Saliva

Riis

Bryce

Matin³

et al. 2018

Biomark. Med.

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Section: Resultssupporting

confidence: 55%

The Validity, Stability, and Utility of Measuring Uric Acid in Saliva

Riis

Bryce

Matin³

et al. 2018

Biomark. Med.

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“…При этом выработка BDNF стволовыми клетками пародонтальной связки снижалась по мере старения, что сопровождалось уменьшением экспрессии остеогенных пептидов с увеличением маркеров клеточного старения [11]. Оценка содержания NGF-β в РЖ отличалась аналитическими сложностями, поскольку основным источником данного нейротрофина в РЖ являются слюнные железы, продуцирующие преимущественно проформу NGF, а не зрелый NGF-β [12]. Вероятно, низкая концентрация исследованных нейротрофинов в РЖ при возраст-ассоциированных заболеваниях полости рта не позволила установить ожидаемые взаимосвязи.…”

Section: обсуждение полученных данныхunclassified

Neurotrophins and vascular endothelial growth factor in oral fluid of elderly patients: diagnostic value for chronic periodontitis and oral lichen planus

Bazarnyi,

Kopenkin,

Polushina

et al. 2024

Tihookeanskij medicinskij žurnal

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Objective. To evaluate the vascular endothelial growth factor A and brain-derived neurotrophic factor in the oral fluid of mature and elderly patients with chronic periodontitis and oral lichen planus. Materials and methods. The study involved 56 participants. The control group consisted of healthy volunteers aged 18–44 (n=10). The comparison group included relatively healthy elderly people aged 60–74 (n=12). The distinguished groups of patients with age-associated dental diseases included: moderate chronic periodontitis mature patients of 45–59 years (n=10) and elderly patients (n=14), as well as elderly patients with oral lichen planus (n=10). The patients underwent dental examination. The content of neurotrophins and vascular endothelial growth factor A in saliva (BDNF/ NGF beta/ VEGF-A Human ProcartaPlex Simplex Kit, Invitrogen, USA) was determined by the multiparametric fluorescence analysis with magnetic microspheres (xMAP, Luminex 200, USA) in compliance with the manufacturer protocol. Results. The groups reveal no differences in the level of brain-derived neurotrophic factor and nerve growth factor beta. The elderly patients with oral lichen planus were found to obtain the highest angiogenesis factor, which diagnostic value was assessed by ROC-analysis. The test appears moderately accurate (AUC=0.875). Conclusion. Vascular endothelial growth factor A can be considered for laboratory monitoring of elderly patients with oral lichen planus.

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“…The ELISA kits used to determine NGF concentrations in saliva [5,6] are not capable of distinguishing between NGF and its pro-forms. In addition, measurement of NGF in saliva is subject to non-specific interference by constituents present in oral fluids [13]. Therefore, values of 1–10 ng/mL of mature NGF in human saliva, as estimated by ELISA, are likely to be too high.…”

Section: Sources Of Nerve Growth Factor (Ngf) In the Oral Cavity Amentioning

confidence: 99%

The Role of Nerve Growth Factor (NGF) and Its Precursor Forms in Oral Wound Healing

Schenck

Schreurs

Hayashi

et al. 2017

IJMS

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Nerve growth factor (NGF) and its different precursor forms are secreted into human saliva by salivary glands and are also produced by an array of cells in the tissues of the oral cavity. The major forms of NGF in human saliva are forms of pro-nerve growth factor (pro-NGF) and not mature NGF. The NGF receptors tropomyosin-related kinase A (TrkA) and p75 neurotrophin receptor (p75NTR) are widely expressed on cells in the soft tissues of the human oral cavity, including keratinocytes, endothelial cells, fibroblasts and leukocytes, and in ductal and acinar cells of all types of salivary glands. In vitro models show that NGF can contribute at most stages in the oral wound healing process: restitution, cell survival, apoptosis, cellular proliferation, inflammation, angiogenesis and tissue remodeling. NGF may therefore take part in the effective wound healing in the oral cavity that occurs with little scarring. As pro-NGF forms appear to be the major form of NGF in human saliva, efforts should be made to study its function, specifically in the process of wound healing. In addition, animal and clinical studies should be initiated to examine if topical application of pro-NGF or NGF can be a therapy for chronic oral ulcerations and wounds.

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Measuring nerve growth factor in saliva by immunoassay: A cautionary note

Cited by 6 publications

References 14 publications

The Validity, Stability, and Utility of Measuring Uric Acid in Saliva

The Validity, Stability, and Utility of Measuring Uric Acid in Saliva

Neurotrophins and vascular endothelial growth factor in oral fluid of elderly patients: diagnostic value for chronic periodontitis and oral lichen planus

The Role of Nerve Growth Factor (NGF) and Its Precursor Forms in Oral Wound Healing

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