Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force microscopy

Barai, Amlan; Das, Alakesh; Sen, Shamik

doi:10.1016/j.xpro.2021.100296

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…Traditional PA hydrogel layers are most commonly fabricated by sandwiching the liquid gel mixture between glass surfaces 52 however silicone gels are sufficiently viscous to enable droplet spin- coating onto glass coverslips, which allows for more precise and reliable control of gel properties such as thickness and surface smoothness. Further, the dependence of PA hydrogels on initiators (ammonium persulfate and TEMED) introduces batch variance in gel polymer lengths and elasticity https://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_1156.pdf.…”

Section: Discussionmentioning

confidence: 99%

Substrate Stiffness Reshapes Layer Architecture and Biophysical Features of Human Induced Pluripotent Stem Cells to Modulate their Differentiation Potential

Llewellyn,

Charrier,

Helfer

et al. 2024

Preprint

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Lineage-specific differentiation of human induced pluripotent stem cells (hiPSCs) relies on complex interactions between biochemical and physical cues. Here we investigated the ability of hiPSCs to undergo lineage commitment in response to inductive signals and assessed how this competence is modulated by substrate stiffness. We showed that Activin A-induced hiPSC differentiation into mesendoderm and its derivative, definitive endoderm is enhanced on gel-based substrates softer than glass. This correlated with changes in tight junction formation and extensive cytoskeletal remodeling. Further, live imaging and in silico studies suggested changes in cell motility, shape, forces and pressures, underlie hiPSC layer reshaping on soft substrates. Finally, we repurposed an ultra-soft silicone gel, which may provide a suitable substrate for culturing hiPSCs at physiological stiffnesses. Our results provide mechanistic insight into how epithelial mechanics dictate the hiPSC response to chemical signals, and provide a tool for their efficient differentiation in emerging stem cell therapies.

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Section: Discussionmentioning

confidence: 99%

Substrate Stiffness Reshapes Layer Architecture and Biophysical Features of Human Induced Pluripotent Stem Cells to Modulate their Differentiation Potential

Llewellyn,

Charrier,

Helfer

et al. 2024

Preprint

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“…By observing the cell surface morphology and three-dimensional structure, quantitative parameters (cell surface area, thickness, width, and volume) are obtained. Some studies have shown that AFM can be used for cancer detection and drug identification. − Considering the clinical treatment of combination chemotherapy, we chose CB[8] with a larger cavity of 367 Å 3 to deliver materials. In this work, we tried to expand the research on the supramolecular approach by using two CB[8] - encapsulated chemotherapeutic drugs (lobaplatin, LP; eptaplatin, EP), which formed the LP + EP@CB[8] (CLE) complex and controlled the release of drugs through a colorectal tumor cell special biomarker.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Combination Chemotherapy: Cucurbit[8]uril Complex Enhanced Platinum Drug Infiltration and Modified Nanomechanical Property of Colorectal Cancer Cells

Zhou

Meng

et al. 2022

Langmuir

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Combination chemotherapy is recognized as a vital medical treatment for cancer, but it has not achieved clinical ideal effects of combination therapy. Herein, we designed a supramolecular combination chemotherapy strategy based on cucurbit[8]uril (CB[8]), which can be facilely assembled into dual platinum drugs. Interestingly, employing the CB[8] carrier led to a greater than 10-fold intracellular Pt content compared to that of dual drugs at 4 h, and the CB[8] complex (CLE) can enhance the infiltration of platinum drugs in colorectal tumor cells tremendously. The platinum drugs can be released from CLE through consuming more tumor biomarker spermidine. Through analyzing the nanomechanical property of the colorectal tumor cellular surface by bioscope AFM, it was revealed that CLE modified the property by decreasing the adhesion and increasing the stiffness. This study provided a facile and sensitive strategy for improving combination chemotherapy by supramolecular materials.

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Therapeutic potential of Bergenin in the management of neurological-based diseases and disorders

Rezaiezadeh,

Langarizadeh,

Tavakoli

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force microscopy

Cited by 8 publications

References 12 publications

Substrate Stiffness Reshapes Layer Architecture and Biophysical Features of Human Induced Pluripotent Stem Cells to Modulate their Differentiation Potential

Substrate Stiffness Reshapes Layer Architecture and Biophysical Features of Human Induced Pluripotent Stem Cells to Modulate their Differentiation Potential

Supramolecular Combination Chemotherapy: Cucurbit[8]uril Complex Enhanced Platinum Drug Infiltration and Modified Nanomechanical Property of Colorectal Cancer Cells

Therapeutic potential of Bergenin in the management of neurological-based diseases and disorders

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