Measuring metabolic rate in the field: the pros and cons of the doubly labelled water and heart rate methods

Abstract

Summary 1.Measuring the metabolic rate of animals in the field (FMR) is central to the work of ecologists in many disciplines. In this article we discuss the pros and cons of the two most commonly used methods for measuring FMR. 2. Both methods are constantly under development, but at the present time can only accurately be used to estimate the mean rate of energy expenditure of groups of animals. The doubly labelled water method (DLW) uses stable isotopes of hydrogen and oxygen to trace the flow of water and carbon dioxide through the body over time. From these data, it is possible to derive a single estimate of the rate of oxygen consumption ( ) for the duration of the experiment. The duration of the experiment will depend on the rate of flow of isotopes of oxygen and hydrogen through the body, which in turn depends on the animal's size, ranging from 24 h for small vertebrates to up to 28 days in Humans. 3. This technique has been used widely, partly as a result of its relative simplicity and potential low cost, though there is some uncertainty over the determination of the standard error of the estimate of mean . 4. The heart rate ( f H ) method depends on the physiological relationship between heart rate and . 5. If these two quantities are calibrated against each other under controlled conditions, f H can then be measured in free-ranging animals and used to estimate . 6. The latest generation of small implantable data loggers means that it is possible to measure f H for over a year on a very fine temporal scale, though the current size of the data loggers limits the size of experimental animals to around 1 kg. However, externally mounted radio-transmitters are now sufficiently small to be used with animals of less than 40 g body mass. This technique is gaining in popularity owing to its high accuracy and versatility, though the logistic constraint of performing calibrations can make its use a relatively extended process.

Limits to sustained energy intake. XIV. Heritability of reproductive performance in mice

“…A known amount of food was also collected to determine the water content of the food. Daily energy expenditure (DEE, kJday −1 ) was measured by the doubly labelled water (DLW) technique from day 14 to day 16 of lactation (over 48h) (Butler et al, 2004;Lifson et al, 1955). This method has been previously validated by comparison to indirect calorimetry in a range of small mammals (Speakman and Król, 2005b).…”

Implantation of subcutaneous heart rate data loggers in southern elephant seals (Mirounga leonina)

“…Measurement of heart rate is commonly used to estimate the metabolic rate of laboratory and free-ranging mammals (Butler et al 2004;Green 2011). In laboratory studies, fully implantable heart rate devices are usually used (Morhardt and Morhardt 1971;Mills et al 2000).…”

The effect of feeding on CO2 production and energy expenditure in ponies measured by indirect calorimetry and the 13C-bicarbonate technique

“…The DLW method is relatively expensive because of the high cost of the isotope 18 O. Moreover, the DLW method is not appropriate in shortterm experiments because of its slow rate of isotope elimination, resulting in a long measuring period of days or weeks (Butler et al, 2004). The 13 C-BT is based on 13 C kinetics in expired CO 2 after administration of 13 C-labelled sodium bicarbonate (NaH 13 CO 3 ).…”