Measuring Haitian children's exposure to chikungunya, dengue and malaria

Poirier, Mathieu J. P.; Moss, Delynn M.; Feeser, Karla; Streit, Thomas G.; Chang, Gwong‐Jen J.; Whitney, Matthew T.; Russell, Brandy J.; Johnson, Barbara W.; Basile, Alison Jane; Goodman, Christin H.; Barry, Amanda; Lammie, Patrick J.

doi:10.2471/blt.16.173252

Cited by 19 publications

(19 citation statements)

References 38 publications

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“…In our study, all age groups living in urban areas showed substantially higher MFI-bg signal intensity (IgG responses) to the CHIKV antigens compared with the same age groups living in rural areas. Differences were not seen between IgG prevalence or titer between younger and older age categories, which is atypical for serologic studies of infectious diseases ( 2 , 9 , 24 , 25 ), because an older age indicates more life-years of potential exposure to an endemic infectious disease and, thus, acquisition of IgG. These serologic findings provide strong evidence for the rapid dissemination of CHIKV by the Aedes vector.…”

Section: Discussionmentioning

confidence: 80%

“…The CHIKV IgG bead assay was designed by CDC laboratories ( 2 ), and the assay was conducted at CDC laboratories (Atlanta, GA, USA). Carboxyl groups on the surface of specifically classified spectral polystyrene microspheres (BioPlex microbeads; Bio-Rad, Hercules, CA, USA) were converted to reactive esters by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method (Calbiochem, Woburn, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…We evaluated the sensitivity and specificity of the bead assay in comparison to the anti-CHIKV IgG ELISA protocol used by CDC’s Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases ( 2 – 4 ). For the ELISA, the viral antigen came from the brain of a suckling mouse and was captured with a monoclonal antibody.…”

Section: Methodsmentioning

confidence: 99%

“…Additional evidence that CHIKV was introduced into Haiti in 2014 came from evaluation of a longitudinal cohort of children in the coastal town of Leogane during 2011–2014. Before 2014, these children tested negative for CHIKV antibodies, but samples collected in 2014 showed CHIKV IgG; 78.9% of all children seroconverted within the span of 1 year ( 2 ).…”

mentioning

confidence: 99%

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Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti

Rogier¹,

Moss²,

Mace³

et al. 2018

Emerg. Infect. Dis.

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The index case of chikungunya virus (CHIKV) in Haiti was reported during early 2014; the vector, the pervasive Aedes aegypti mosquito, promoted rapid spread throughout the country. During December 2014–February 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout Haiti and measured CHIKV IgG by using a multiplex bead assay. Overall CHIKV seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. Logistic modeling identified the urban environment as a strong predictor of CHIKV exposure (adjusted odds ratio 3.34, 95% CI 2.38–4.69), and geographic elevation provided a strong negative correlation. We observed no correlation between age and antibody positivity or titer. Our findings demonstrated through serologic testing the recent and rapid dissemination of the arbovirus throughout the country. These results show the utility of serologic data to conduct epidemiologic studies of quickly spreading mosquitoborne arboviruses.

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Section: Discussionmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti

Rogier¹,

Moss²,

Mace³

et al. 2018

Emerg. Infect. Dis.

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“…Because the multiplexing capacity allows for the simultaneous analysis of up to 100 different antigens from one sample, and because samples can be analyzed off-site in a reference laboratory, Luminex MBAs have been shown to be an effective method for data collection in low-resource settings and at a low cost per antigen analyzed. Previous studies have used Luminex MBAs to detect serum antibody responses to tuberculosis, lymphatic filariasis, chikungunya, dengue, malaria, and enteric protozoa ( Giardia intestinalis , Entamoeba histolytica , and Cryptosporidium parvum ) [ 25 – 27 , 30 – 32 ].…”

Section: Introductionmentioning

confidence: 99%

The impact of school water, sanitation, and hygiene improvements on infectious disease using serum antibody detection

et al. 2018

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BackgroundEvidence from recent studies assessing the impact of school water, sanitation and hygiene (WASH) interventions on child health has been mixed. Self-reports of disease are subject to bias, and few WASH impact evaluations employ objective health measures to assess reductions in disease and exposure to pathogens. We utilized antibody responses from dried blood spots (DBS) to measure the impact of a school WASH intervention on infectious disease among pupils in Mali.Methodology/Principal findingsWe randomly selected 21 beneficiary primary schools and their 21 matched comparison schools participating in a matched-control trial of a comprehensive school-based WASH intervention in Mali. DBS were collected from 20 randomly selected pupils in each school (n = 807). We analyzed eluted IgG from the DBS using a Luminex multiplex bead assay to 28 antigens from 17 different pathogens. Factor analysis identified three distinct latent variables representing vector-transmitted disease (driven primarily by dengue), food/water-transmitted enteric disease (driven primarily by Escherichia coli and Vibrio cholerae), and person-to-person transmitted enteric disease (driven primarily by norovirus). Data were analyzed using a linear latent variable model. Antibody evidence of food/water-transmitted enteric disease (change in latent variable mean (β) = -0.24; 95% CI: -0.53, -0.13) and person-to-person transmitted enteric disease (β = -0.17; 95% CI: -0.42, -0.04) was lower among pupils attending beneficiary schools. There was no difference in antibody evidence of vector-transmitted disease (β = 0.11; 95% CI: -0.05, 0.33).Conclusions/SignificanceEvidence of enteric disease was lower among pupils attending schools benefitting from school WASH improvements than students attending comparison schools. These findings support results from the parent study, which also found reduced incidence of self-reported diarrhea among pupils of beneficiary schools. DBS collection was feasible in this resource-poor field setting and provided objective evidence of disease at a low cost per antigen analyzed, making it an effective measurement tool for the WASH field.Trial registrationThe trial was registered at ClinicalTrials.gov (NCT01787058)

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Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

Priest

Moss

2019

Methods in Molecular Biology

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Measuring Haitian children's exposure to chikungunya, dengue and malaria

Cited by 19 publications

References 38 publications

Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti

Use of Bead-Based Serologic Assay to Evaluate Chikungunya Virus Epidemic, Haiti

The impact of school water, sanitation, and hygiene improvements on infectious disease using serum antibody detection

Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

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