Measuring Erythrocyte Morphology by Electron Microscopy to Diagnose Hematuria

Максимов, Г. В.; Mamaeva, S.N.; Antonov, S. R.; Munkhalova, Ya.A.; Kononova, I.V.; Sheikin, I. Yu.

doi:10.1007/s11018-016-0966-5

Cited by 10 publications

(8 citation statements)

References 2 publications

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“…Due to the fact, that the GO substrates contain oxygen groups, the total electrical conductivity of the system increases by their removal and introduction of silver (Ag) nanoparticles. Earlier, we proved that with the help of electron microscopy it becomes possible to reveal changes in the morphology of erythrocytes in pathology and is likely we can investigate the presences of biological particles (viruses) on the surface of red cell [1][2][3].…”

Section: Kne Energy and Physicsmentioning

confidence: 93%

“…The presence of such nanoobjects on the surface of erythrocytes was observed in patients with diseases of Berger and other types of nephropathy [2,3], as well as for patients with cervical cancer. Figure 5 shows images of the surface of a red blood cell sample of a girl of 10 years with chronic glomerulonephritis at magnifications of 50,000× on a substrate of glass.…”

Section: Physbiosymp17mentioning

confidence: 94%

“…Currently using a new class of electron microscopes to study the morphology of biological objects at low voltages and without metal deposition of the object [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

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Use of Nanocomposite Material Based on Graphene Oxide and Silver Nanoparticles in Research of Blood Erythrocytes in Various Diseases

Mamaeva¹,

Максимов²,

Neustroev³

et al. 2018

KEn

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The electrical and structural properties of a nanocomposite material based on silver nanoparticles and graphene oxide were investigated. Using electron and atomic force microscopy it was shown that the nanoparticles formed predominantly have sizes from 60 to 100 nm. Measurements of the current-voltage characteristics showed electrical conductivity values significantly exceeding the parameters of the initial graphene oxide. The prepared nanocomposites were used for scanning by electron microscopy (SEM) of blood samples of sick children with hematuria syndrome and patients of the radiological department of an oncologic dispensary diagnosed with cervical cancer. The formation of nanosize objects on the surface of erythrocytes is revealed. The size of these volumes is comparable to the size of the viruses. Results of these studies can indirectly confirm an assumption of authors about possible transportation of viruses by erythrocytes to various organs and viral etiology of renal diseases with the hematuria syndrome and cervical cancer.

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Section: Kne Energy and Physicsmentioning

confidence: 93%

Section: Physbiosymp17mentioning

confidence: 94%

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Use of Nanocomposite Material Based on Graphene Oxide and Silver Nanoparticles in Research of Blood Erythrocytes in Various Diseases

Mamaeva¹,

Максимов²,

Neustroev³

et al. 2018

KEn

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“…Poikilocytosis is the term used for abnormal shaped RBC in the blood (4). Assessing alterations in red blood cell morphology provides important information that can help to establish a differential diagnosis of diseases in both humans and animals (5)(6)(7)(8). In addition to diseases, other physiological factors can cause changes in erythrocyte morphology, such as aging (9,10).…”

Section: Introductionmentioning

confidence: 99%

Red blood cells morphology and morphometry in adult, senior, and geriatricians dogs by optical and scanning electron microscopy

et al. 2022

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Red blood cells (RBC) morphologic evaluation through microscopy optical (OM) and SEM, provides information to forecast, evaluate, and monitor the functioning of many organs. Factors, such aging and diseases affect RBC morphology in both, human and animals. SEM is useful to evaluate RBC morphology, although its use in diagnosis and evaluation in dogs is limited, due to the availability and cost. The aim of this research was to assess the normal RBC morphology in adult, senior and geriatrician dogs, clinically healthy by OM and SEM. In addition to evaluating the age effect, sex, body size, and their interaction on erythrocyte morphometry. To carry out the research 152 blood samples were evaluated from dogs of different sexes and body sizes (small, medium, and large). Three groups were made based on dogs age: group I adults (1–7.9 years old), group II senior (8–11.9 years old), and group III geriatricians (>12 years old). Erythrocyte parameters were evaluated by OM (diameter, height, and axial ratio). Per each dog, the parameters of 20 erythrocytes were measured. A total of 2,600 cells were scanned with the AmScope™ Software scale. In addition, the RBC morphology was evaluated by SEM. Statistical analyses used analysis of variance and a general linear model, which allows the comparison of multiple factors at two or more levels (p < 0.05). The results of this study showed that diameter and height were lower in adult dogs than in senior and geriatrician dogs (p < 0.05). Whereas, sex, body size, and the interaction did not show a significant effect (p > 0.05). Additionally, some images of anisocytosis, polychromasia, and poikilocytosis (echinocytes, acanthocytes, codocytes, spherocytes, stomatocytes, dacryocytes quatrefoil, and elliptocytes) were obtained by OM and SEM. Our study provides information about the morphological and morphometry alterations of adult, senior, and geriatrician dogs RBC. This work contributes to future investigations and the diagnosing diseases, where it is necessary to evaluate the morphology of RBC.

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“…Одним из таких исследований является изучение Ig-нефропатий у детей, которое проводилось на базе нефрологического отделения Педиатрического центра Республиканской больницы № 1-НЦМ. Было обследовано 30 детей с гематурическим синдромом, где предварительные данные показали, что кроме дисморфных эритроцитов наблюдается наличие сопутствующих объектов нанометровых размеров, сопоставимых с размерами малых вирусов, что может подтвердить предположения о вирусном этиологическом факторе в возникновении болезни Берже [11,12,13].…”

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Междисциплинарные Подходы В Медицинской Науке

Слепцова¹,

Максимова²,

Саввина³

et al. 2022

Вестник Северо-Восточного Федерального Университета Имени М К Аммосова Серия "Медицинские Науки

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МЕЖДИСЦИПЛИНАРНЫЕ ПОДХОДЫ В МЕДИЦИНСКОЙ НАУКЕАннотация. Инновационные разработки в медицине могут быть продуктивными только при использовании комплексного мультидисциплинарного подхода. Решение современных вызовов в медицинской науке требует новых подходов и решений. И это не только взаимодействие и взаимодополнение специальностей медицинского направления, но и решение проблем со смежными науками, такими как физика,

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Measuring Erythrocyte Morphology by Electron Microscopy to Diagnose Hematuria

Cited by 10 publications

References 2 publications

Use of Nanocomposite Material Based on Graphene Oxide and Silver Nanoparticles in Research of Blood Erythrocytes in Various Diseases

Use of Nanocomposite Material Based on Graphene Oxide and Silver Nanoparticles in Research of Blood Erythrocytes in Various Diseases

Red blood cells morphology and morphometry in adult, senior, and geriatricians dogs by optical and scanning electron microscopy

Междисциплинарные Подходы В Медицинской Науке

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