Measuring Endocrine (Cortisol) Responses of Zebrafish to Stress

Canavello, Peter R.; Cachat, Jonathan; Beeson, Esther; Laffoon, Autumn L.; Grimes, Chelsea; Haymore, Whitlee A.M.; Elegante, Marco; Bartels, Brett; Hart, Peter C.; Elkhayat, Salem; Tien, David; Mohnot, Sopan; Amri, Hakima; Kalueff, Allan V.

doi:10.1007/978-1-60761-953-6_11

Cited by 35 publications

(29 citation statements)

References 15 publications

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“…Cortisol levels were measured according to a protocol previously described by Canavello et al (2010). Briefly, frozen fish were weighed and pulverised in liquid nitrogen.…”

Section: Full-body Cortisol Measurementmentioning

confidence: 99%

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Tudorache¹,

Schaaf²,

Slabbekoorn³

2013

Journal of Endocrinology

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All vertebrates exhibit physiological responses to a wide variety of stressors. The amplitude and profile of the response depend on the intensity, duration, controllability and predictability of the stressor, but there is also individual variation in the response, termed coping style. A better understanding of the expression of coping styles is of great value for medical applications, animal welfare issues and conservation. Here, we investigated the effect of repeated netting stress on proactive and reactive zebrafish (Danio rerio) as an upcoming model system for stress research. Fish were separated by coping styles according to the order of entering a novel environment. Subsequently, repeated netting stress was applied as stressor, over a period of 21 days. Full-body cortisol levels were determined at 0, 15, 30, 60 and 120 min after the last repeated stress event. Our results show that reactive fish display i) increased basal cortisol concentrations after being repeatedly stressed, ii) higher cortisol secretion over time and iii) slow recovery of cortisol concentration towards basal levels after the last repeated stress event. This study shows for the first time in zebrafish that different coping styles are associated with different cortisol responses during the recovery from stress over time and that coping styles can explain otherwise unaccounted variation in physiological stress responses.

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“…Cortisol levels were measured according to a protocol previously described by Canavello et al (2010). Briefly, frozen fish were weighed and pulverised in liquid nitrogen.…”

Section: Full-body Cortisol Measurementmentioning

confidence: 99%

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Tudorache¹,

Schaaf²,

Slabbekoorn³

2013

Journal of Endocrinology

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“…Notably, altered c-fos brain expression and cortisol/corticosterone levels have been reported in various mammalian models following hallucinogenic drugs, including LSD, 163,164 MDMA, 165,166 ketamine, 167,168 and PCP. 113,169 Zebrafish display a welldeveloped neuroendocrine system 74,170 and show high sensitivity of their CNS proto-oncogene expression to various experimental manipulations. 87,144,171 From this point of view, the sensitivity of zebrafish physiological biomarkers to hallucinogenic drugs (Table 2) supports the utility of such aquatic models to parallel behavioral phenotypes with physiological responses.…”

Section: ■ Modeling Hallucinogenic Drug Action In Zebrafishmentioning

confidence: 99%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

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Among different classes of psychotropic drugs, hallucinogenic agents exert one of the most prominent effects on human and animal behaviors, markedly altering sensory, motor, affective, and cognitive responses. The growing clinical and preclinical interest in psychedelic, dissociative, and deliriant hallucinogens necessitates novel translational, sensitive, and high-throughput in vivo models and screens. Primate and rodent models have been traditionally used to study cellular mechanisms and neural circuits of hallucinogenic drugs' action. The utility of zebrafish (Danio rerio) in neuroscience research is rapidly growing due to their high physiological and genetic homology to humans, ease of genetic manipulation, robust behaviors, and cost effectiveness. Possessing a fully characterized genome, both adult and larval zebrafish are currently widely used for in vivo screening of various psychotropic compounds, including hallucinogens and related drugs. Recognizing the growing importance of hallucinogens in biological psychiatry, here we discuss hallucinogenic-induced phenotypes in zebrafish and evaluate their potential as efficient preclinical models of drug-induced states in humans.

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“…Enzyme‐linked immunosorbent assay (competitive ELISA) for human salivary cortisol (Salimetrics; http://www.salimetrics. com) modified for whole body D. rerio (Canavello et al ., ) was used to quantify cortisol. Absorbances from reactions were measured at 450 nm using a BioTek Synergy HTX plate reader (http://www.biotek.com).…”

Section: Methodsmentioning

confidence: 99%

“…Whole‐body cortisol extraction was adapted from the method described by Canavello et al . (). Individual fish were weighed, partially thawed to allow efficient mincing, then homogenized in 1 ml of ice‐cold phosphate buffered saline (PBS) using a Cole‐Parmer LabGen 125 homogenizer (http://www.coleparmer.co.uk) in 5 × 15 s bursts followed by homogenizer wash with an additional 2 × 1 ml PBS.…”

Section: Methodsmentioning

confidence: 99%

Olfactorily‐mediated cortisol response to chemical alarm cues in zebrafish Danio rerio

Barkhymer

Garrett

Wisenden

2018

Journal of Fish Biology

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Chemical cues released as a by‐product of predation mediate antipredator behaviour, but little is known about the physiological responses to olfactory detection of predation risk. In this study, zebrafish Danio rerio were exposed to either chemical alarm cues from conspecifics, or water (control). Compared with water controls, D. rerio exposed to alarm cues responded behaviourally with antipredator behaviours such as erratic dashing and an increase in time spent near the bottom of the test aquarium. Danio rerio were sacrificed 5 min after exposure to test cues (alarm cues or water). Enzyme‐linked immunosorbent assay (ELISA) revealed whole‐body levels of cortisol that were significantly higher for fish exposed to alarm cues (mean ± SE, 11.9 ± 3.4 ng g−1) than control fish (1.5 ± 0.7 ng g−1). These data provide a benchmark for future studies of the proximate mechanisms of olfactorily mediated antipredator responses, modelling effects on aquatic life in a changing climate and, as a model organism, Danio rerio can further our understanding of anxiety in humans.

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Measuring Endocrine (Cortisol) Responses of Zebrafish to Stress

Cited by 35 publications

References 15 publications

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Olfactorily‐mediated cortisol response to chemical alarm cues in zebrafish Danio rerio

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