Measuring Donnan-related phenomena using a solid-state ion sensor and a concentration-step method

Eijkel, Jan C.T.; Olthuis, Wouter; Kolev, Spas D.; Bergveld, Piet

doi:10.1016/s0376-7388(96)00281-5

Cited by 7 publications

(8 citation statements)

References 21 publications

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“…Eijkel et al [28] measured Donnan-related phenomena with the use of a miniature device (sensor % 1 mm) consisting of an ISFET (ion selective field effect transistor) pH-sensor together with a Ag/AgCl electrode, covered by a microporous composite membrane. The latter two electrochemical approaches to measure Donnan potentials are by no means simplistic.…”

Section: Introductionmentioning

confidence: 99%

“…They are designed for specific uses (e.g., in muscle fibers, biosensors for protein detection and immunosensing) and are not easily adopted for more general application. Furthermore, they require specialized expertise and the interpretation of the results is not straightforward [28]. Additionally, these particular electrochemical approaches are not able to detect the concentration of metal cations.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of Donnan potentials in gels by in situ microelectrode voltammetry

Davis

Yezek

Pinheiro

et al. 2005

Journal of Electroanalytical Chemistry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Measurement of Donnan potentials in gels by in situ microelectrode voltammetry

Davis

Yezek

Pinheiro

et al. 2005

Journal of Electroanalytical Chemistry

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“…[50,[52][53][54][55] At any time, t, after application of the ion-step, the concentration of a migrating species (e.g., H þ ) at the membrane/ISFET interface c t is given by Morf [56] : Following an ion step there will be a change in interface potential according to the above equation (giving the measured response) and a redistribution of all ions due to the new Donnan ratio, starting at the membrane solution interface.…”

Section: A Methods For Detection Of Pmb Release From Polymersmentioning

confidence: 99%

Testing the Durability of Polymyxin B Immobilization on a Polymer Showing Antimicrobial Activity: A Novel Approach with the Ion-Step Method

et al. 2003

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A polymyxin B (PMB) modified copolymer of ethyl acrylate and 2hydroxyethyl methacrylate is reported that shows antibacterial activity towards E. coli. The polymer is designed for biosensor interfaces to provide protection against microbial contamination. However, leaching of a biomolecule from its solid support is a well-known problem in immobilization chemistry. It has been proposed that antimicrobial activity results from release of the antibiotic from the polymer matrix, but in the work reported here, bactericidal action was seen for tresyl activated polymers even when no polymyxin B could be detected in the supernatant and where covalent attachment of PMB was indicated. Further evidence for the in situ action of PMB on the polymer was provided using a novel method of investigation, subjecting the polymer, deposited on an Ion-Sensitive Field-Effect Transistor (ISFET) to a step change in ion-concentration (Ion-step). With the ion-step method activation of the polymer and the grafting of Polymyxin-B were followed. A comparison of absorbed PMB on a nonactivated polymer and PMB on the tresyl activated polymer suggested that if leaching of PMB from the latter occurred, it was below the detection limit and well below the minimum inhibitory concentration (MIC) for antimicrobial action. Bactericidal action was thus attributed to PMB attached to the polymer.

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“…1 shows mixing/reaction curves calculated using a finite-element solution procedure. 37 The curves show the light emission per volume unit and per second as a function of the time passed since the start of mixing.…”

Section: A B Light + Ae Ae Ae Kmentioning

confidence: 99%