Measuring Dielectrophoresis Force for Metallic and Non-metallic Particle Manipulations via a Quartz Tuning Fork Atomic Force Microscope

Hong, Sung-Hoon; Kim, Chungman; Song, Hansol; An, Sangmin; Yoon, Hosang; Jhe, Wonho

doi:10.3938/jkps.75.1021

Cited by 3 publications

(1 citation statement)

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“…The forces imposed on the particle in a field generated by interdigitated electrodes numerically [26,27] and analytically using the Fourier series have been calculated [28]. Other DEP force measurement approaches include atomic force microscopy [29] and optical tweezers [30]. Measuring the accurate amount of the DEP force when imposed on sperm is essential to the design and optimization of the working conditions of actuators that use this phenomenon.…”

Section: Introductionmentioning

confidence: 99%

Enhancing the Accuracy of Measuring DEP Force Applied on Cells by Considering the Friction Effect

et al. 2023

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The Dielectrophoresis (DEP) phenomenon has been widely used for cell separation in recent years. The experimental measurement of the DEP force is one of the concerns of scientists. This research presents a novel method for more accurately measuring the DEP force. The innovation of this method is considered the friction effect, which has been neglected in previous studies. For this purpose, first, the direction of the microchannel was aligned with the electrodes. As there was no DEP force in this direction, the release force of the cells caused by the fluid flow equaled the friction force between the cells and the substrate. Then, the microchannel was aligned perpendicular to the direction of the electrodes, and the release force was measured. The net DEP force was obtained by the difference between the release forces of these two alignments. In the experimental tests, the DEP force, when applied to the sperm and white blood cell (WBC), was measured. The WBC was used to validate the presented method. The experimental results showed that the forces applied by DEP to WBC and human sperm were 42 pN and 3 pN, respectively. On the other hand, with the conventional method, these figures were as high as 72 pN and 4 pN due to neglecting the friction force. The compression between the simulation results in COMSOL Multiphysics and the experiments determined the new approach to be valid and capable of use in any cell, such as sperm.

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Section: Introductionmentioning

confidence: 99%

Enhancing the Accuracy of Measuring DEP Force Applied on Cells by Considering the Friction Effect

et al. 2023

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Dielectrophoresis: Measurement technologies and auxiliary sensing applications

Hu,

Ji,

Chen

et al. 2024

Electrophoresis

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Dielectrophoresis (DEP), which arises from the interaction between dielectric particles and an aqueous solution in a nonuniform electric field, contributes to the manipulation of nano and microparticles in many fields, including colloid physics, analytical chemistry, molecular biology, clinical medicine, and pharmaceutics. The measurement of the DEP force could provide a more complete solution for verifying current classical DEP theories. This review reports various imaging, fluidic, optical, and mechanical approaches for measuring the DEP forces at different amplitudes and frequencies. The integration of DEP technology into sensors enables fast response, high sensitivity, precise discrimination, and label‐free detection of proteins, bacteria, colloidal particles, and cells. Therefore, this review provides an in‐depth overview of DEP‐based fabrication and measurements. Depending on the measurement requirements, DEP manipulation can be classified into assistance and integration approaches to improve sensor performance. To this end, an overview is dedicated to developing the concept of trapping‐on‐sensing, improving its structure and performance, and realizing fully DEP‐assisted lab‐on‐a‐chip systems.

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On-chip dielectrophoretic single-cell manipulation

Tian,

Wang,

Chen

2024

Microsyst Nanoeng

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Bioanalysis at a single-cell level has yielded unparalleled insight into the heterogeneity of complex biological samples. Combined with Lab-on-a-Chip concepts, various simultaneous and high-frequency techniques and microfluidic platforms have led to the development of high-throughput platforms for single-cell analysis. Dielectrophoresis (DEP), an electrical approach based on the dielectric property of target cells, makes it possible to efficiently manipulate individual cells without labeling. This review focusses on the engineering designs of recent advanced microfluidic designs that utilize DEP techniques for multiple single-cell analyses. On-chip DEP is primarily effectuated by the induced dipole of dielectric particles, (i.e., cells) in a non-uniform electric field. In addition to simply capturing and releasing particles, DEP can also aid in more complex manipulations, such as rotation and moving along arbitrary predefined routes for numerous applications. Correspondingly, DEP electrodes can be designed with different patterns to achieve different geometric boundaries of the electric fields. Since many single-cell analyses require isolation and compartmentalization of individual cells, specific microstructures can also be incorporated into DEP devices. This article discusses common electrical and physical designs of single-cell DEP microfluidic devices as well as different categories of electrodes and microstructures. In addition, an up-to-date summary of achievements and challenges in current designs, together with prospects for future design direction, is provided.

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Measuring Dielectrophoresis Force for Metallic and Non-metallic Particle Manipulations via a Quartz Tuning Fork Atomic Force Microscope

Cited by 3 publications

References 22 publications

Enhancing the Accuracy of Measuring DEP Force Applied on Cells by Considering the Friction Effect

Enhancing the Accuracy of Measuring DEP Force Applied on Cells by Considering the Friction Effect

Dielectrophoresis: Measurement technologies and auxiliary sensing applications

On-chip dielectrophoretic single-cell manipulation

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