Measuring copepod naupliar abundance in a subtropical bay using quantitative PCR

Jungbluth, Michelle J.; Goetze, Erica; Lenz, Petra H.

doi:10.1007/s00227-013-2300-y

Cited by 18 publications

(15 citation statements)

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“…The parasitic copepods reported here were almost certainly collected as pelagic nauplii, the life history phase that disperses and locates new hosts. Approximately 50% of the specimens collected by our plankton pumps were nauplii (Kersten, 2015); however, nauplii are extremely challenging to identify morphologically (Jungbluth, Goetze & Lenz, 2013), and no prior deep-sea plankton studies have attempted taxonomic assignment of nauplii (e.g., Christiansen et al, 1999; Wishner, 1980; Kersten, Smith & Vetter, 2017). We combined a 63 µm mesh size for collections with metabarcoding analyses, enabling us to detect this highly diverse and almost entirely unknown component of the meroplanktonic fauna.…”

Section: Discussionmentioning

confidence: 99%

Larval assemblages over the abyssal plain in the Pacific are highly diverse and spatially patchy

Kersten

Vetter

Jungbluth

et al. 2019

PeerJ

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Abyssal plains are among the most biodiverse yet least explored marine ecosystems on our planet, and they are increasingly threatened by human impacts, including future deep seafloor mining. Recovery of abyssal populations from the impacts of polymetallic nodule mining will be partially determined by the availability and dispersal of pelagic larvae leading to benthic recolonization of disturbed areas of the seafloor. Here we use a tree-of-life (TOL) metabarcoding approach to investigate the species richness, diversity, and spatial variability of the larval assemblage at mesoscales across the abyssal seafloor in two mining-claim areas in the eastern Clarion Clipperton Fracture Zone (CCZ; abyssal Pacific). Our approach revealed a previously unknown taxonomic richness within the meroplankton assemblage, detecting larvae from 12 phyla, 23 Classes, 46 Orders, and 65 Families, including a number of taxa not previously reported at abyssal depths or within the Pacific Ocean. A novel suite of parasitic copepods and worms were sampled, from families that are known to associate with other benthic invertebrates or demersal fishes as hosts. Larval assemblages were patchily distributed at the mesoscale, with little similarity in OTUs detected among deployments even within the same 30 × 30 km study area. Our results provide baseline observations on larval diversity prior to polymetallic nodule mining in this region, and emphasize our overwhelming lack of knowledge regarding larvae of the benthic boundary layer in abyssal plain ecosystems.

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Section: Discussionmentioning

confidence: 99%

Larval assemblages over the abyssal plain in the Pacific are highly diverse and spatially patchy

Kersten

Vetter

Jungbluth

et al. 2019

PeerJ

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“…Instead, we have to rely on genetic tools being developed. A promising method is the use of quantitative PCR to identify and estimate the abundance of nauplii from mixed samples (Jungbluth et al ).…”

Section: Discussionmentioning

confidence: 99%

“…nauplii are often pooled, as they are morphologically identical (Sømme ) and data on length distributions are rare and derived from different areas. Quantitative genetic methods have been used to estimate biomass of nauplii stages of a single‐target species (Jungbluth et al ) in a mixed sample, but genetic analyses of natural communities are time consuming and costly.…”

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confidence: 99%

Early development of Calanus glacialis and C. finmarchicus

Jung-Madsen

Nielsen

2015

Limnology & Oceanography

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Egg and nauplii development of coexisting populations of Calanus glacialis and C. finmarchicus from Disko Bay, western Greenland, were measured in the laboratory with, and without addition of food. Egg hatching rate was measured at five temperatures from 0 C to 10 C and the fit to a Bel ehr adek equation was highly significant (r 2 > 0.99). There was little difference between hatching and development rates of the two species; however, the egg hatching rate at low temperatures was faster than predicted from measurements made in other areas. Also, nauplii development times at 0 C were similar, with C. glacialis and C. finmarchicus reaching nauplii stage N6 after 44 d and 48 d, respectively. The laboratory-reared nauplii could be separated accurately to species by stage and length alone. However, separation by size was not possible for the in situ community of nauplii where the influence of food, temperature, and size-dependent predation mortality, made the size distribution more variable and overlapping.The Arctic environment is warming with reduced sea ice cover and accelerated melting of inland glaciers. These changes in forcing factors alter the physical settings for the biological processes and succession in the ocean (IPCC 2013). Therefore, a major effort has been made to investigate and predict how temperature changes impact the vulnerable Arctic marine ecosystems.

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“…As expected a $3 Ct units increase corresponded to each 10 fold dilution (e.g. Albaina et al, 2010;Jungbluth et al, 2013). The high sensibility of the assay allowed detecting DNA extracted from a single anchovy egg.…”

Section: Assay Validationmentioning

confidence: 95%

A real-time PCR assay to estimate invertebrate and fish predation on anchovy eggs in the Bay of Biscay

Albaina

Irigoien

Aldalur

et al. 2015

Progress in Oceanography

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Measuring copepod naupliar abundance in a subtropical bay using quantitative PCR

Cited by 18 publications

References 100 publications

Larval assemblages over the abyssal plain in the Pacific are highly diverse and spatially patchy

Larval assemblages over the abyssal plain in the Pacific are highly diverse and spatially patchy

Early development of Calanus glacialis and C. finmarchicus

A real-time PCR assay to estimate invertebrate and fish predation on anchovy eggs in the Bay of Biscay

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