Measuring citrate synthase activity as an enzymatic approach to the differentiation of chilled and frozen/thawed meat

Škorpilová, Tereza; Šístková, Iveta; Adamcová, Markéta; Pohůnek, Václav; Kružík, Vojtěch; Ševčík, Rudolf

doi:10.1016/j.meatsci.2019.05.027

Cited by 14 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, SUCLA 2 rebound increases, pleiotropically affecting metabolic pathways associated with SUCL [31]. CS synthesizes isocitrate from oxaloacetate, and Tereza Škorpilová, et al showed that there could be speci c limits designed for CS activity in chilled and frozen/thawed meats [32]. DLST catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO 2 [33].…”

Section: Discussionmentioning

confidence: 99%

A Label-Free Proteomic Strategy to Investigate the Intramuscular fat Proteomic Differences Between Biceps Femoris and Longissimus Dorsi in Inner Mongolian Cashmere Goats

Xie

Liu

Nai

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: As a major raw-cashmere-producing province in China. Nearly 700,000 Aerbasi cashmere goats are fed per year, and the corresponding meat production is nearly 10,000 tons. However, there are no reports on the meat of this goat. To better understand the molecular variations underlying intramuscular fat (IMF) anabolism and catabolism in Inner Mongolian cashmere goats, the proteomic differences between the biceps femoris (BF) and longissimus dorsi (LD) were investigated by a label-free strategy. Then, the identified proteins were verified as being involved in IMF anabolism and catabolism by Western blot analysis.Results: The IMF content was significantly higher in the BF than in the LD, suggesting that IMF accumulated more in the BF or was metabolized more in the LD. We performed proteomic analysis of IMF anabolism and catabolism at the proteomic level, and 1209 proteins were identified in the BF (high-IMF) and LD (low-IMF) groups. Among them, 110 were differentially expressed proteins (DEPs), 81 of which were upregulated in the high-IMF group, while 29 were upregulated in the low-IMF group. Gene ontology (GO) classification showed that the 110 DEPs were functionally classified into 100 annotation clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the 110 DEPs covered 34 KEGG pathways. Three pathways were related to IMF metabolism and deposition—fatty acid metabolism, fatty acid degradation and fatty acid elongation—and included 7 proteins.Conclusion: GO and KEGG analyses showed that differentially expressed HADHA, HADHB, ACSL1, ACADS, ACAT1 and ACAA2 in the mitochondria act via fatty acid metabolism, fatty acid degradation and fatty acid elongation to influence the metabolism and synthesis of long-, short- and medium-chain fatty acids and modulate IMF anabolism and catabolism. Protein-protein interaction (PPI) network analysis showed that IMF accumulation in different muscle tissues of Inner Mongolian cashmere goats was affected not only by 5 key enzymes and proteins involved in fatty acid synthesis and metabolism but also by five DEPs (SUCLG1, SUCLG2, CS, DLST, and ACO2) in the TCA cycle. Our results provide new insights into IMF deposition in goats and improve our understanding of the molecular mechanisms underlying IMF anabolism and catabolism.

show abstract

Section: Discussionmentioning

confidence: 99%

A Label-Free Proteomic Strategy to Investigate the Intramuscular fat Proteomic Differences Between Biceps Femoris and Longissimus Dorsi in Inner Mongolian Cashmere Goats

Xie

Liu

Nai

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…65 Citrate synthase activity assay: The activity of citrate synthase was measured as described. 66 In brief, 100 midguts of sugar fed mosquitoes administrated with/without serotonin were dissected in cool PBS and homogenized in 200 μl lysis buffer (0.25% TritonX-100/PBS) at 4°C. After 1: 2 dilution in lysis buffer, 40 μl of the lysate mixed with 60 μl reaction buffer (0.25% TritonX-100/PBS, 0.31mM acetyl CoA, 0.1mM DTNB and 0.5mM oxaloacetate).…”

Section: Starmethodsmentioning

confidence: 99%

Host 5-HT affectsPlasmodiumtransmission in mosquitoes via modulating mosquito mitochondrial homeostasis

Gao,

Zhang,

Feng

et al. 2024

Preprint

View full text Add to dashboard Cite

SUMMARYMalaria parasites hijack the metabolism of their mammalian host during the blood-stage cycle.Anophelesmosquitoes depend on mammalian blood to survive and to transmit malaria parasites. However, it remains understudied whether changes in host metabolism affect parasite transmission in mosquitoes. In this study, we discovered thatPlasmodiuminfection significantly decreased the levels of the tryptophan metabolite, 5-hydroxytryptamine (5-HT), in both humans and mice. The reduction led to the decrease of 5-HT in mosquitoes. Oral supplementation of 5-HT toAnopheles stephensienhanced its resistance toPlasmodium bergheiinfection by promoting the generation of mitochondrial reactive oxygen species. This effect was due to the accumulation of dysfunctional mitochondria caused by 5-HT-mediated inhibition of mitophagy. Elevating 5-HT levels in mouse serum significantly suppressed parasite infection in mosquitoes. In summary, our data highlight the critical role of metabolites in animal blood in determining the capacity of mosquitoes to control parasite infection.

show abstract

“…Several research studies report on the use of the mitochondrial enzymes β‐hydroxyacyl coenzyme A dehydrogenase (HADH) (Garcia de Fernando et al., 1992; Hoz et al., 1992, 1993; Pavlov et al., 1994; Civera et al., 1996; Fernandez et al., 1999; Duflos et al., 2002; Bernardi et al., 2019) as a successful means of differentiation. Other enzyme assays involve mitochondrial citrate synthase (Skorpilova et al., 2019), succinate dehydrogenase (Civera et al., 1996) and the lysosomal enzymes β‐N-acetylglucosaminidase extracted from fish muscle (Rehbein et al., 1978; Rehbein, 1979; Nilsson and Ekstrand, 1993; Duflos et al., 2002; Alberio et al., 2014) or fish blood (Kitamikado et al, 1990; Yuan et al, 1988); α‐glucosidase (Rehbein et al., 1978; Rehbein, 1979; Nilsson and Ekstrand, 1993; Rehbein and Cakli, 2000; Duflos et al., 2002; Marlard et al., 2019); β‐galactosidase , β‐glucuronidase (Rehbein, 1979) and acid phosphatase (Rehbein, 1979; Nilsson and Ekstrand, 1993). The cytosolic enzyme lactate dehydrogenase has also been tested (Rehbein, 1979; Diop et al., 2016).…”

Section: Assessmentmentioning

confidence: 99%

The use of the so‐called ‘superchilling’ technique for the transport of fresh fishery products

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2021

EFS2

View full text Add to dashboard Cite

Superchilling entails lowering the fish temperature to between the initial freezing point of the fish and about 1–2°C lower. The temperature of superchilled fresh fishery products ( SFFP ) in boxes without ice was compared to that of products subject to the currently authorised practice in boxes with ice ( CFFP ) under the same conditions of on‐land storage and/or transport. A heat transfer model was developed and made available as a tool to identify under which initial configurations of SFFP the fish temperature, at any time of storage/transport, is lower or equal to CFFP . A minimum degree of superchilling, corresponding to an ice fraction in the fish matrix of SFFP equal or higher than the proportion of ice added per mass of fish in CFFP , will ensure with 99–100% certainty (almost certain) that the fish temperature of SFFP and the consequent increase of relevant hazards will be lower or equal to that of CFFP . In practice, the degree of superchilling can be estimated using the fish temperature after superchilling and its initial freezing point, which are subject to uncertainties. The tool can be used as part of ‘safety‐by‐design’ approach, with the reliability of its outcome being dependent on the accuracy of the input data. An evaluation of methods capable of detecting whether a previously frozen fish is commercially presented as ‘superchilled’ was carried out based on, amongst others, their applicability for different fish species, ability to differentiate fresh fish from fish frozen at different temperatures, use as a stand‐alone method, ease of use and classification performance. The methods that were considered ‘fit for purpose’ are Hydroxyacyl‐coenzyme A dehydrogenase ( HADH ) test, α‐glucosidase test, histology, ultraviolet–visible–near–infrared ( UV ‐ VIS / NIR ) spectroscopy and hyperspectral imaging. These methods would benefit from standardisation, including the establishment of threshold values or classification algorithms to provide a practical routine test.

show abstract

Measuring citrate synthase activity as an enzymatic approach to the differentiation of chilled and frozen/thawed meat

Cited by 14 publications

References 19 publications

A Label-Free Proteomic Strategy to Investigate the Intramuscular fat Proteomic Differences Between Biceps Femoris and Longissimus Dorsi in Inner Mongolian Cashmere Goats

A Label-Free Proteomic Strategy to Investigate the Intramuscular fat Proteomic Differences Between Biceps Femoris and Longissimus Dorsi in Inner Mongolian Cashmere Goats

Host 5-HT affectsPlasmodiumtransmission in mosquitoes via modulating mosquito mitochondrial homeostasis

The use of the so‐called ‘superchilling’ technique for the transport of fresh fishery products

Contact Info

Product

Resources

About