Measuring Cellular Ploidy In Situ by Light Microscopy

Clay, Delisa E; Stormo, Benjamin M; Fox, Donald T.

doi:10.1007/978-1-0716-2561-3_21

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Briefly, tissue was fixed in 4% paraformaldehyde + 0.3% Triton X-100. Ploidy Analysis of Drosophila Cardiomyocytes: Cardiomyocyte ploidy measurements were done as previously described (Clay et al, 2023). Briefly, the larval cardiac organ was dissected in 1X PBS and fixed with 4% formaldehyde solution.…”

Section: Methodsmentioning

confidence: 99%

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Conserved Chamber-Specific Polyploidy Maintains Heart Function inDrosophila

Chakraborty

Peterson

King

et al. 2023

Preprint

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Developmentally programmed polyploidy (whole-genome-duplication) of cardiomyocytes is common across evolution. Functions of such polyploidy are essentially unknown. Here, we reveal roles for precise polyploidy levels in cardiac tissue. We highlight a conserved asymmetry in polyploidy level between cardiac chambers in Drosophila larvae and humans. In Drosophila, differential Insulin Receptor (InR)sensitivity leads the heart chamber to reach a higher ploidy/cell size relative to the aorta chamber. Cardiac ploidy-reduced animals exhibit reduced heart chamber size, stroke volume, cardiac output, and acceleration of circulating hemocytes. These Drosophila phenotypes mimic systemic human heart failure. Using human donor hearts, we reveal asymmetry in nuclear volume (ploidy) and insulin signaling between the left ventricle and atrium. Our results identify productive and likely conserved roles for polyploidy in cardiac chambers and suggest precise ploidy levels sculpt many developing tissues. These findings of productive cardiomyocyte polyploidy impact efforts to block developmental polyploidy to improve heart injury recovery.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Cardiomyocyte ploidy measurements were done as previously described (Clay et al, 2023). Briefly, the larval cardiac organ was dissected in 1X PBS and fixed with 4% formaldehyde solution.…”

Section: Methodsmentioning

confidence: 99%

Conserved Chamber-Specific Polyploidy Maintains Heart Function inDrosophila

Chakraborty

Peterson

King

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Conserved chamber-specific polyploidy maintains heart function in Drosophila

et al. 2023

View full text Add to dashboard Cite

Developmentally programmed polyploidy (whole-genome-duplication) of cardiomyocytes is common across evolution. Functions of such polyploidy are essentially unknown. Here, in both Drosophila larvae and human organ donors, we reveal distinct polyploidy levels in cardiac organ chambers. In Drosophila, differential growth and cell cycle signal sensitivity leads the heart chamber to reach a higher ploidy/cell size relative to the aorta chamber. Cardiac ploidy-reduced animals exhibit reduced heart chamber size, stroke volume, cardiac output, and acceleration of circulating hemocytes. These Drosophila phenotypes mimic human cardiomyopathies. Our results identify productive and likely conserved roles for polyploidy in cardiac chambers and suggest precise ploidy levels sculpt many developing tissues. These findings of productive cardiomyocyte polyploidy impact efforts to block developmental polyploidy to improve heart injury recovery.

show abstract

Measuring Cellular Ploidy In Situ by Light Microscopy

Cited by 2 publications

References 14 publications

Conserved Chamber-Specific Polyploidy Maintains Heart Function inDrosophila

Conserved Chamber-Specific Polyploidy Maintains Heart Function inDrosophila

Conserved chamber-specific polyploidy maintains heart function in Drosophila

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