Measuring autofluorescence spectral signatures for detecting antibiotic-resistant bacteria using Thermofisher’s Bigfoot spectral flow cytometer
Sharath Narayana Iyengar,
Valery Patsekin,
Bartek Rajwa
et al.
Abstract:Application of flow cytometry to microbiology has been limited due to inadequate availability of bacterial-specific stains, expensive antibody-based fluorophores, ineffective stain cell permeability and challenges in differentiating bacterial cells from cell debris due to their similarity and their small size. Moreover, conventional staining methods require multiple washing steps, limiting sensitivity due to bacterial cells' significantly smaller volume compared to eukaryotic cells. Additionally, most flow cyt… Show more
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